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Availability & Fees Order this Strain
Homozygous: Animals with the Mesdc2-floxed allele are viable and fertile. No overt phenotype observed.
Heterozygous: Animals with the Mesdc2-floxed allele are viable and fertile. No overt phenotype observed.
If cre-mediated recombinase is activated: PGK-Cre excision of Mesdc2 in all tissues results in a null phenotype indistinguishable from the conventional Mesdc2 knockout or albino deletion strain. Excision of Mesdc2 in adult liver hepatocytes using adenovirus-cre was effective in inducing recombination; the phenotype was not characterized.
The Mesd conditional knockout allele (C57BL/6-Mesdc2tm2Bch (Mesd-floxed)) was generated by Ozgene. The targeting construct was electroporated into ES cell line, Bruce4 derived from B6.Cg-Thy1a. Homologous recombinant ES cells were injected into blastocysts from B6N-Tyrc-Brd albino mice. Chimeras were mated to B6N-albino, and resulting heterozygous progeny were backcrossed to C56BL/6J.Backcross progeny heterozygotes were intercrossed to generate Mesd-Floxed homozygotes and were maintained by intercrossing.
Taibi AV, Lighthouse JK, Grady RC, Shroyer KR, Holdener BC. Development of a conditional Mesd (mesoderm development) allele for functional analysis of the low-density lipoprotein receptor-related family in defined tissues. PLoS One. 2013 Oct 4;8(10):e75782. (Medline PMID: 24124512)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.