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Availability & Fees Order this Strain
This is a congenic strain by serial backcrossing to introgress a chromosomal fragment containing the Tas1r3 allele from the high sweetener-preferring C57BL/6ByJ (B6ByJ) strain onto the genetic background of the low sweetener-preferring 129P3/J strain. The congenic interval is on chromosome 4 and is 194 kb. It begins proximally somewhere between markers 280G12-T7 and 4902-T7 (155,171,951-155,187,450 bp) and ends distally somewhere between markers 350D2-T7 and D4Mon1 (155,302,732-155,365,331 bp). Tas1r3 is at 155,233,379-155,237,462 bp.
Homozygous: Mice with the Tas1r3Sac-b allele have higher behavioral and neural taste responses to sweeteners than mice with the Tas1r3Sac-d allele.
Heterozygous: Tas1r3Sac-b allele is dominant. Therefore, mice with the one or two Tas1r3Sac-b alleles have higher behavioral and neural taste responses to sweeteners than mice with two Tas1r3Sac-d alleles.
Li X, Inoue M, Reed DR, Huque T, Puchalski RB, Tordoff MG, Ninomiya Y, Beauchamp GK, Bachmanov AA. High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal chromosome 4. Mamm Genome. 2001 Jan;12(1):13-6. (Medline PMID: 11178737)
Bachmanov AA, Li X, Reed DR, Ohmen JD, Li S, Chen Z, Tordoff MG, de Jong PJ, Wu C, West DB, Chatterjee A, Ross DA, Beauchamp GK. Positional cloning of the mouse saccharin preference (Sac) locus. Chem Senses. 2001 Sep;26(7):925-33. (Medline PMID: 11555487)
Inoue M, Glendinning JI, Theodorides ML, Harkness S, Li X, Bosak N, Beauchamp GK, Bachmanov AA. Allelic variation of the Tas1r3 taste receptor gene selectively affects taste responses to sweeteners: evidence from 129.B6-Tas1r3 congenic mice. Physiol Genomics. 2007 Dec 19;32(1):82-94. Epub 2007 Oct 2. (Medline PMID: 17911381)
Tordoff MG, Shao H, Alarcon LK, Margolskee RF, Mosinger B, Bachmanov AA, Reed DR, McCaughey S. Involvement of T1R3 in calcium-magnesium taste. Physiol Genomics. 2008 Aug 15;34(3):338-48. Epub 2008 Jul 1. (Medline PMID: 18593862)
Colony Surveillance Program and Current Health Reports
Wild-type (129P3/J) female x Heterozygous male or reciprocal mating
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.