Strain Name    : B6.FVB(Cg)-Tg(Dlg3-cre)KG118Gsat/Mmucd

Stock Number : 036967-UCD

Gene Information

(provided by MGI)
Transgene: Tg(Dlg3-cre)KG118Gsat
Name: transgene insertion KG118, GENSAT Project at Rockefeller University
Alteration at locus: Transgenic (cre)
Transgene: cre (Bacteriophage P1)
Name: cre recombinase
Alteration at locus: Transgenic (cre)
Promoter: Dlg3
Name: discs, large homolog 3 (Drosophila)
Alteration at locus: Transgenic (cre)
Genetic Alterations:
Using GENSAT BAC engineering system, the Cre recombinase gene was introduced upstream of the ATG start codon of the gene of interest on a selected BAC clone. The inserted gene has its own ATG start codon and polyadenylation signal. This modification results in the expression of Cre recombinase that is under the control of the promoter and regulatory elements of the gene of interest that are present on the BAC clone. The BAC address for this line is RP23-474H6.

SNP analysis has shown the transgenic insertion to be on chromosome 15 between SNPs rs3707900 and rs13482637.

Genotype Determination:

ES Cell Line:

Phenotype

This congenic strain carries the same mutation as MMRRC:032809 and was originally characterized on a STOCK background before backcrossing to C57BL/6J for at least 10 generations. It should be noted that the phenotype could vary from that originally described. BAC-transgenic images of the central nervous system at various ages of the transgenic mouse can be found on the GENSAT project page. In situ histochemistry images using the BGEM probe are also located on the GENSAT website.

Mammalian Phenotype Terms:

Strain genetic background: B6.FVB(Cg)

Strain Development: The original transgenic mice were created in the GENSAT project in collaboration with the Intramural Program of the National Institute of Mental Health. The transgenic mice have a modified BAC-cre injected into pronuclei of FVB/N fertilized oocytes. Offspring of founders were crossed to a Soriano Rosa26/stop/EGFP reporter line. The MMRRC backcrossed this transgene from the mixed background to C57BL/6J, selected for mice with only the cre expression, and eliminated the Rosa26-GFP from the line. Mice were determined to be congenic on C57BL/6J by SNP analysis.

Suggested Control Mice: Wild-type littermates or C57BL/6J mice

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  • Neurobiology
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Donor: MMRRC at University of California, Davis.  
Original strain (MMRRC:032809) donated by Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT

Primary Reference:

  • Gong S, Doughty M, Harbaugh CR, Cummins A, Hatten ME, Heintz N, Gerfen CR. Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs. J Neurosci. 2007 Sep 12;27(37):9817-23. No abstract available. (Medline PMID: 17855595).
  • Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature. 2003 Oct 30;425(6961):917-25. (Medline PMID: 14586460). 

Colony and Husbandry Information

In general, GENSAT BAC transgenic mouse lines should be utilized in the hemizygous state. Breeding of the BAC transgene to a homozygous state carries an increased risk of generating an unwanted phenotype for two reasons. First, the transgene inserts at random into the genome. Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. Second, BAC transgenes may carry passenger genes that are also expressed from the BAC vector. Breeding to homozygosity doubles the number of these genes carried in the transgenic mouse, increasing the possibility that a phenotype will result as a consequence of increased gene dosage. In our experience, observable phenotypes are rare in BAC transgenic mice when carried in the hemizygous state.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: Black

Other:

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • Wild-type (C57BL/6J) female x Hemizygous Tg male or reciprocol mating
  • Wild-type female x Hemizygous Tg male sibling or reciprocol mating

Generation: N10. Strain was determined to be congenic on C57BL/6J by SNP analysis.

Overall Breeding Performance: Good

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undertimed Undertermind

Average litter size: 6

Recommended wean age: 3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
036967-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
036967-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.