Strain Name:
B6N.129S6-Dbitm1Kier/Mmjax
Stock Number:
036975-JAX
Citation ID:
RRID:MMRRC_036975-JAX
Other Names:
ACBP knock-out, ACBP-null, Dbi-null, Dbi-KO

Gene Information

Dbitm1Kier
Name: diazepam binding inhibitor; targeted mutation 1, Ann Kier
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Dbi
Name: diazepam binding inhibitor
Synonyms: diazepam-binding inhibitor, Acbp, EP, endozepine
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 13167
HGNC: HGNC:2690
Homologene: 39086
Genetic Alterations
Exon 1, intron 1, exon 2, and part of intron 2 of Dbi gene were replaced with a neomycin cassette.
Genotype Determination
ES Cell Line
W4 derived from 129S6/SvEvTac
Phenotype

Homozygous: The ACBP-null mutation is embryonically lethal at the early pre-implantation stage. Homozygote ACBP-null emrbyos fail to develop past the morula stage.
Heterozygous: Mice were not characterized but appear normal.

Mammalian Phenotype Terms
Allelic Composition: Dbitm1Kier/Dbitm1Kier (Genetic Background: B6NCr.129S6-Dbitm1Kier )

MeSH Terms
  • Acyl Coenzyme A/metabolism
  • Animals
  • Diazepam Binding Inhibitor
  • Embryo, Mammalian/metabolism
  • Embryonic Development/genetics
  • Female
  • Gene Deletion
  • Genes, Lethal
  • Male
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Pregnancy
Strain Development
Correctly targeted W4 ES-cells were injected into C57BL/6NCr blastocysts and chimera males were breed to C57BL/6NCr females resulting in F1 progeny. The heterozygote F1 progeny were subsequently bred to C57BL/6NCr mice for 10 generation with at least one breeding of heterozygous females to C57BL/6NCr males to turn the Y-chromosome. After 10 generations of backcrossing, the colony was maintained by inbreeding.
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Metabolism
  • Obesity
Donor
Ann Kier, D.V.M., Ph.D., Texas A&M University, College of Veterinary Medicine and Biomedcial Sciences.
Primary Reference

Landrock D, Atshaves BP, McIntosh AL, Landrock KK, Schroeder F, Kier AB. Acyl-CoA binding protein gene ablation induces pre-implantation embryonic lethality in mice. Lipids. 2010 Jul;45(7):567-80. (Medline PMID: 20559753)

Colony and Husbandry Information

Background strain is C57BL/6NCr, to prevent excesive canibalization or neglect breeding was supported by use of fostering.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)

Wild-type (C57BL/6NCr) female x Heterozygous male or reciprocal mating

Wild-type female x Heterozygous male littermate or reciprocal mating

Homozygous female x Homozygous male littermate

Generation
N10F3
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 10 months
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036975-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
036975-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.