Strain Name:
B6N.129P2-Scp2tm1Kier/Mmjax
Stock Number:
036977-JAX
Citation ID:
RRID:MMRRC_036977-JAX
Other Names:
SCP-x-null, SCP-x<-/->, Scp2-exon1-KO

Gene Information

Scp2tm1Kier
Name: sterol carrier protein 2, liver; targeted mutation 1, Ann B Kier
Synonyms: SCP-x-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Scp2
Name: sterol carrier protein 2, liver
Synonyms: SCPx, nonspecific lipid transfer protein, NSL-TP, ns-LTP, SCP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
NCBI: 20280
Homologene: 37717
Genetic Alterations
Exon 1 of Scp2 was replaced with a neomycin resistance cassette. Disruption of exon 1 of Scp2 results in a complete ablation of Scp-x but not Scp-2.
ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype

Homozygous: mice show abnormal liver physiology and lipid metabolism.

Heterozygous: mice were not sufficiently characterized; appear to be normal.

Mammalian Phenotype Terms
Allelic Composition: Scp2tm1Kier/Scp2tm1Kier (Genetic Background: involves: 129P2/OlaHsd * C57BL/6NCr )

MeSH Terms
  • Acetyl-CoA C-Acetyltransferase/genetics
  • Acetyl-CoA C-Acetyltransferase/metabolism
  • Alkyl and Aryl Transferases/genetics
  • Alkyl and Aryl Transferases/metabolism
  • Animals
  • Blotting, Western
  • Body Fat Distribution
  • Body Weight/drug effects
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Fatty Acid Transport Proteins/metabolism
  • Fatty Acids/analysis
  • Fatty Acids/metabolism
  • Female
  • Gene Expression Regulation, Enzymologic
  • Lipid Metabolism/drug effects
  • Lipids/analysis
  • Lipids/blood
  • Lipids/chemistry
  • Liver/drug effects
  • Liver/metabolism
  • Liver/pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mitochondria/drug effects
  • Mitochondria/enzymology
  • Organ Size/drug effects
  • Peroxisomes/drug effects
  • Peroxisomes/enzymology
  • Phytanic Acid/metabolism
  • Phytol/metabolism
  • Phytol/pharmacology
  • Sex Factors
Strain Development
E14 ES-cells carrying the Scp2 mutation were injected into C57BL/6 blastocysts. Germline transmission was tested by breeding the chimeras produced by the injection to C57BL/6 females. F1 heterozygous mice were backcrossed for 10 genereations to C57BL/6NCr, with at least one breeding involving wild type C57BL/6NCr males to turn the Y-chromosome. After backcrossing colony was maintained by inbreeding. At N10F5 mice were backcrossed for one genereation to C57BL/6NCr, after which sib-mating resumed. At N11F8 another backcrossing was performed, current generation is N12.
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Cell Biology
  • Diabetes
  • Metabolism
  • Obesity
Donor
Ann Kier, D.V.M., Ph.D., Texas A&M University, College of Veterinary Medicine and Biomedcial Sciences.
Primary Reference

Atshaves BP, McIntosh AL, Landrock D, Payne HR, Mackie JT, Maeda N, Ball J, Schroeder F, Kier AB. Effect of SCP-x gene ablation on branched-chain fatty acid metabolism. Am J Physiol Gastrointest Liver Physiol. 2007 Mar;292(3):G939-51. (Medline PMID: 17068117)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)

Wild-type (C57BL/6NCr) female x Heterozygous male or reciprocal mating

Wild-type female x Heterozygous male littermate or reciprocal mating

Homozygous female x Homozygous male littermate

Generation
N12+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 10 months
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036977-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
036977-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.