Strain Name:
C3Fe.B6-Golga3repro27/LincMmjax
Stock Number:
037050-JAX
Citation ID:
RRID:MMRRC_037050-JAX
Other Names:
C3-27, C3Fe-repro27

Gene Information

Golga3repro27
Name: golgi autoantigen, golgin subfamily a, 3; reproductive mutant 27, JAX Reproductive Mutagenesis Program
Synonyms: G1-499-14
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
Golga3
Name: golgi autoantigen, golgin subfamily a, 3
Synonyms: Mea-2, Mea2, 5430416E01Rik, G1-499-14, repro27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 269682
HGNC: HGNC:4426
Homologene: 4308
Genetic Alterations
Premature termination codon in exon 18 of Golga3
Genotype Determination
Phenotype

Homozygous: Males are sterile due to defects in germ cell development, germ cell death due to apoptosis.

Heterozygous: Fertile

Mammalian Phenotype Terms
Allelic Composition: Golga3repro27/Golga3repro27 (Genetic Background: C3Fe.B6-Golga3repro27 )

Allelic Composition: Golga3repro27/Golga3repro27 (Genetic Background: involves: C3HeB/FeJ * C57BL/6J )

MeSH Terms
  • Animals
  • Autoantigens/genetics
  • Male
  • Membrane Proteins/genetics
  • Mice
  • Point Mutation
  • Spermatogenesis/genetics
Strain Development
ENU induced mutation in C57BL/6J bred with C3HeB/FeJ then backcrossed for 10 generations to make congenic on C3HeB/FeJ background. Colony maintained with intercross breeding.
Suggested Control Mice
Wild-type littermates or C3HeB/FeJ mice
  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
    • Male infertility
  • Reproduction
Donor
Carol Linder , Ph.D., New Mexico Highlands University.
Primary Reference

Bentson LF, Agbor VA, Agbor LN, Lopez AC, Nfonsam LE, Bornstein SS, Handel MA, Linder CC. New point mutation in Golga3 causes multiple defects in spermatogenesis. Andrology 2013 May;1(3):440-50, Epub 2013 Mar 15. (Medline PMID: 23495255)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Agouti
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)

Mutant female x heterozygous male, homozygous mutant males are sterile.

Generation
10
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Sterile
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037050-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
037050-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.