Gene Symbol: Pthlh
Name: parathyroid hormone-like peptide
Alteration at locus: Targeted Mutation
Name: parathyroid hormone-like peptide; targeted mutation 1, Arthur E Broadus
Alteration at locus: Targeted Mutation
ES Cell Line: W9.5/W95 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Homozygous: A short-limbed chondrodystrophy that is a neonatal lethal caused by a mineralized shield chest and respiratory failure. The homozygous KO was subsequently rescued via a collagen-2-driven Pthlh replacement stragegy. The rescued mouse has a failure of tooth eruption, total failure of mammary epithelial development and other developemental abnormalties.
Heterozygous: Pthlh-driven lacZ expression
Cre-excised Phenotype: MMTV-Cre in mammary gland Sclerasis-Cre in periosteum and tendon/ligament insertion site in bone Rin-1-Cre in pancreatic B cells
Strain of Origin: 129S1/Sv-Oca2+ Tyr+ Kitl+
Strain genetic background: CD1.129S1(B6)
Strain Development: Correctly targeted W9.5 ES cells derived from 29S1/Sv-Oca2+ Tyr+ Kitl+ were microinjected into C57BL/6 blastocysts. Chimeras were bred to C57BL/6J, then outbred onto CD1 for three or more generations. The donor reports that the mice submitted to the MMRRC have been outcrossed to CD1 for 30+ generations.
Suggested Control Mice: Wild-type littermates
Donor: Arthur Broadus, M.D., Ph.D., Yale University.
Coat color: White
MMRRC Breeding System: Outcross to non-inbred (hybrid or outbred) mate
Wild-type (CD1) female x Heterozygous male or reciprocal mating.
Outbred to CD1 for more than 30 generation
Overall Breeding Performance: Excellent
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||10 months||6 months|
Average litter size: 10-14
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.