Strain Name    : CD1.129S1(B6)-Pthlhtm1Broa/Mmucd

Stock Number : 037092-UCD

Other Names   : PTHrP-lacZ

Gene Information

(provided by MGI)
Gene Symbol: Pthlh
Name: parathyroid hormone-like peptide
Chromosome: 6
Alteration at locus: Targeted Mutation
Reporter: Pthlhtm1Broa
Name: parathyroid hormone-like peptide; targeted mutation 1, Arthur E Broadus
Chromosome: 6
Alteration at locus: Targeted Mutation
Genetic Alterations:
The lacZ reporter construct gives rise to a biscistronic mRNA under the control of endogenous Pthlh regulatory sequences. The truncated functionless Pthlh product ends in pre-pro sequences, and the translated lacZ product has the kinetics of the lacZ mRNA ( i.e., the AU motifs of the Pthlh mRNA that confer a rapid turnover were eliminated).

Genotype Determination:

ES Cell Line: W9.5/W95 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+

Phenotype

Homozygous: A short-limbed chondrodystrophy that is a neonatal lethal caused by a mineralized shield chest and respiratory failure. The homozygous KO was subsequently rescued via a collagen-2-driven Pthlh replacement stragegy. The rescued mouse has a failure of tooth eruption, total failure of mammary epithelial development and other developemental abnormalties.

Heterozygous: Pthlh-driven lacZ expression

Cre-excised Phenotype: MMTV-Cre in mammary gland Sclerasis-Cre in periosteum and tendon/ligament insertion site in bone Rin-1-Cre in pancreatic B cells

Strain of Origin: 129S1/Sv-Oca2+ Tyr+ Kitl+

Strain genetic background: CD1.129S1(B6)

Strain Development: Correctly targeted W9.5 ES cells derived from 29S1/Sv-Oca2+ Tyr+ Kitl+ were microinjected into C57BL/6 blastocysts.  Chimeras were bred to C57BL/6J, then outbred onto CD1 for three or more generations.  The donor reports that the mice submitted to the MMRRC have been outcrossed to CD1 for 30+ generations.

Suggested Control Mice: Wild-type littermates

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
    • Blomstrand chondrodysplasia 
  • Research Tools

Donor: Arthur Broadus, M.D., Ph.D., Yale University.

Primary Reference:

  • Chen X, Macica CM, Dreyer BE, Hammond VE, Hens JR, Philbrick WM, Broadus AE. Initial characterization of PTH-related protein gene-driven lacZ expression in the mouse. J Bone Miner Res. 2006 Jan;21(1):113-23. (Medline PMID: 16355280)
  • Macica C, Liang G, Nasiri A, Broadus AE.Genetic evidence of the regulatory role of parathyroid hormone-related protein in articular chondrocyte maintenance in an experimental mouse model. Arthritis Rheum. 2011 Nov;63(11):3333-43. (Medline PMID:21702022)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: White

Other:

MMRRC Breeding System: Outcross to non-inbred (hybrid or outbred) mate

Breeding Scheme(s):

  • Wild-type (CD1) female x Heterozygous male or reciprocal mating.

Generation:

Outbred to CD1 for more than 30 generation

Overall Breeding Performance: Excellent

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 months 6 months

Average litter size: 10-14

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
037092-UCD-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
037092-UCD-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.