Allele Symbol: Ccl2tm1.1Tyos
Name: targeted mutation 1.1, Teizo Yoshimura
Alteration at locus: Knockout
Gene Symbol: Ccl2
Name: chemokine (C-C motif) ligand 2
Alteration at locus: Knockout
ES Cell Line: CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Homozygous/Heterozygous: No observable phenotype.
Expected Phenotype After Cre-excision: Not characterized.
Strain of Origin: 129S1/Sv-Oca2+ Tyr+ Kitl+
Strain genetic background: C.129S1(B6)
The targeted CJ7 ES cells derived from 129S1/Sv-Oca2+ Tyr+ Kitl+ were injected into C57BL/6 blastocysts, and chimeras were bred to C57BL/6 mice to achieve the germline transmission of the Ccl2 mutated allele. Hetrozygous mice were intercrossed Ccl2 to generate homozygous mice (Ccl2neo/neo), which were subsequently crossed to EIIaCre mice to obtain mice lacking exons 1 and 2 of the Ccl2 gene. The genetic background of Ccl2 mice was converted to a BALB/c background using speed congenics.
Suggested Control Mice: Wild-type littermates or BALB/c mice
Donor: Teizo Yoshimura, M.D., Ph.D., National Cancer Institute.
Coat color: White
MMRRC Breeding System: Random intra-strain mating
Overall Breeding Performance: Excellent
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||6 months||6 months|
Average litter size: 5-9
Recommended wean age: 4 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.