Strain Detail Sheet

Strain Name    :

C.129S1(Cg)-Ccl2tm1.1Tyos/Mmnc

Stock Number :

037094-UNC

Other Names   :

MCP-1 KO

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Ccl2tm1.1Tyos
Name: targeted mutation 1.1, Teizo Yoshimura
Chromosome: 11
Alteration at locus: Knockout
Gene Symbol: Ccl2
Name: chemokine (C-C motif) ligand 2
Chromosome: 11
Alteration at locus: Knockout

Genetic Alterations:
The exon 2 of the Ccl2 (MCP1), gene is deleted using the Cre-loxP system.

Genotype Determination:

    ES Cell Line: CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+

    Strain Description [Including phenotype, strain background, strain development and suggested control mice]

    Phenotype

    Homozygous/Heterozygous: No observable phenotype.

    Expected Phenotype After Cre-excision: Not characterized.

    Strain of Origin: 129S1/Sv-Oca2+ Tyr+ Kitl+

    Strain genetic background: C.129S1(B6)

    Strain Development:

    The targeted CJ7 ES cells derived from 129S1/Sv-Oca2+ Tyr+ Kitlwere injected into C57BL/6 blastocysts, and chimeras were bred to C57BL/6 mice to achieve the germline transmission of the Ccl2 mutated allele. Hetrozygous mice  were intercrossed Ccl2 to generate homozygous mice (Ccl2neo/neo), which were subsequently crossed to EIIaCre mice to obtain mice lacking exons 1 and 2 of the Ccl2 gene. The genetic background of Ccl2 mice was converted to a BALB/c background using speed congenics.

    Suggested Control Mice: Wild-type littermates or BALB/mice

    Research Applications

    • Cancer
    • Cardiovascular
    • Dermatology
    • Diabetes
    • Immunology and Inflammation
    • Models for Human Disease
      • Atherosclerosis
      • Rheumatoid arthritis
      • Breast cancer
    • Neurobiology
    • Obesity
    • Research Tools

    Strain Origin

    Donor: Teizo Yoshimura, M.D., Ph.D., National Cancer Institute.

    Primary Reference:

    • Yoshimura T, Howard OM, Ito T, Kuwabara M, Matsukawa A, Chen K, Liu Y, Liu M, Oppenheim JJ, Wang JM. Monocyte Chemoattractant Protein-1/CCL2 Produced by Stromal Cells Promotes Lung Metastasis of 4T1 Murine Breast Cancer Cells. PLoS ONE 8(3).  (Medline PMID: 23527025)
    • Takahashi M, Galligan C, Tessarollo L, Yoshimura T. Monocyte chemoattractant protein-1 (MCP-1), not MCP-3, is the primary chemokine required for monocyte recruitment in mouse peritonitis induced with thioglycollate or zymosan A. J Immunol. 2009 Sep 1;183(5):3463-71. (Medline PMID:19641140)

    Colony and Husbandry Information

    Special Considerations

    None

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat color: White

    Other:

    Breeding

    MMRRC Breeding System: Random intra-strain mating

    Breeding Scheme(s):

    • Wild-type female x Heterozygous male from the colony or reciprocal mating
    • Homozygous female x Homozygous male from the colony

    Generation:

    Overall Breeding Performance: Excellent

    Reproductive Statistics

    Viability and Fertility:FemaleMale
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes

    Age Reproductive Decline: 6 months 6 months

    Average litter size: 5-9

    Recommended wean age: 4 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    037094-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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    Generated: 04/18/2015