Strain Detail Sheet

Strain Name    :

C57BL/6J-Pcdh15m3Btlr/Mmmh

Stock Number :

037097-MU

Other Names   :

mcduck

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Pcdh15m3Btlr
Name: mutation 3, Bruce Beutler
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Pcdh15
Name: protocadherin 15
Chromosome: 10
Alteration at locus: Chemically Induced

Genetic Alterations:
The mcduck mutation is a T to A transversion at base pair 74,626,844 on chromosome 10, corresponding to base pair 1,526,630 in GenBank genomic region NC_000076 encoding Pcdh15. The mutation is located in the donor splice site of intron 33, two nucleotides from the previous exon. Pcdh15 contains 35 total exons. The effect of the mutation at the cDNA and protein level is unknown. One possibility, shown below, is that aberrant splicing may result in the skipping of the 156 base pair exon 33 and splicing from exon 32 to exon 34. The aberrant splicing would lead to a deletion of 52 amino acids as well as a frameshift and subsequent coding of a premature stop codon at amino acid 1506. The protein and mRNA levels in mcduck have not been tested. This model was generated at UT Southwestern Medical Center at Dallas.

Genotype Determination:

ES Cell Line:

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous: Mcduck was initially identified among N-ethyl-N-nitrosourea (ENU)-induced G3 animals as a mutant that displayed hyperactivity and circling with frequent upward head bobbing. Homozygotes also wobble while walking.

Heterozygous: Not characterized.

 

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.

Suggested Control Mice: Wild-type littermates or C57BL/6J mice

Research Applications

  • Cell Biology
  • Neurobiology
  • Sensorineural

Strain Origin

Donor: Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference:

Daniel C, Purrington T, Murray AR, and Beutler B. Record for mcduck. MUTAGENETIX™, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Random intra-strain mating

Breeding Scheme(s):

  • Wild-type female x Heterozygous male from the colony or reciprocal mating
  • Homozygous female x Homozygous male from the colony (not recommended as pups may be trampled before weaning)

Generation:

Overall Breeding Performance: Good.  

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: Undetermined Undetermined

Average litter size: 4-6

Recommended wean age: 3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, see Fees section for further details.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
037097-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
037097-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 03/27/2015