Strain Name:
B6.129S1-Aqp9tm1Nlsn/Mmjax
Stock Number:
037111-JAX
Citation ID:
RRID:MMRRC_037111-JAX
Other Names:
AQP9 KO

Gene Information

Aqp9tm1Nlsn
Name: aquaporin 9; targeted mutation 1, Soren Nielsen
Synonyms: Aqp9-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Aqp9
Name: aquaporin 9
Synonyms: 1700020I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
NCBI: 64008
HGNC: HGNC:643
Homologene: 41405
Genetic Alterations
In the Aqp9 targeted allele, 55 nucleotides in exon 2 were exchanged for a sequence encoding for the neomycin phosphotransferase expression cassette. This insertion is predicted to result in correct translation of the initial 47 amino acids of the AQP9 protein, followed by 100 random amino acids and a stop codon. An aberrantly spliced transcript was detected, where exon 1 was fused to exon 3, however, the production of protein from this transcript is unlikely.
Genotype Determination
ES Cell Line
CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Phenotype

Homozygous: Mild phenotype, elevated plasma glycerol.

Heterozygous: No observable phenotype.

Mammalian Phenotype Terms
Allelic Composition: Aqp9tm1Nlsn/Aqp9tm1Nlsn (Genetic Background: involves: 129S1/Sv )

Allelic Composition: Aqp9tm1Nlsn/Aqp9tm1Nlsn (Genetic Background: involves: 129S1/Sv * C57BL/6 )

MeSH Terms
  • Animals
  • Aquaporins/genetics
  • Aquaporins/metabolism
  • Blood Glucose
  • DNA Primers
  • Fasting/metabolism
  • Glycerol/blood
  • Glycerol/metabolism
  • Immunoblotting
  • Immunohistochemistry
  • Liver/metabolism
  • Mice
  • Mice, Knockout
  • Reverse Transcriptase Polymerase Chain Reaction
  • Triglycerides/blood
Strain Development

The targeting construct was linearized by using NotI and electroporated into CJ7 embryonic stem cells derived from 129S1/Sv-Oca2+ Tyr+ Kitl+ mice. G418-resistant colonies were selected and expanded. Correctly targeted ES cells were injected into B6D2F2 mouse blastocysts. The chimeric males were bred with C57BL/6 females. Offspring were then backcrossed on C57BL/6 at least 10 generations, and maintained as homozygous sibling pairing.

Suggested Control Mice
Wild-type littermates or C57BL/6 mice
  • Diabetes
  • Metabolism
  • Obesity
Donor
Aleksandra Rojek, Ph.D., Aarhus University.
Primary Reference

Rojek A, Skowronski MT, Füchtbauer EM, Füchtbauer AC, Fenton RA, Agre P, Frøkiaer J, Nielsen S. Defective glycerol metabolism in aquaporin 9 (AQP9) knockout mice. Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3609-14. (Medline PMID:17360690).

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Backcross: 
    • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
  • Sib-mating:
    • Wild-type female x Heterozygous male littermate or reciprocal mating
    • Homozygous female x Homozygous male littermate
Generation
N10
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037111-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
037111-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.