Strain Name:
B6;129P2-Drd1tm1Stl/Mmjax
Stock Number:
037113-JAX
Citation ID:
RRID:MMRRC_037113-JAX
Other Names:
dopamine D1 receptor mutant mice

Strain Information

Drd1tm1Stl
Name: dopamine receptor D1; targeted mutation 1, Susumu Tonegawa
Synonyms: D1R-, D1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
Drd1
Name: dopamine receptor D1
Synonyms: Drd1a, D1 receptor, Drd-1, C030036C15Rik, Gpcr15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
NCBI: 13488
VEGA: 13
HGNC: HGNC:3020
Homologene: 30992
Genetic Alterations

Oligonucleotide primers with sequences chosen from the rat and human D1 gene sequences were used to clone a piece of the mouse Drd1a gene. This targeting construct deletes 95% of the D1 gene coding sequence and replaces it with a neo gene. 

Genotype Determination
ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype

Homozygous: Reduced body weight and no longer interested in cocaine.  Homozygote animals show some pre-wean loss and those that survive weaning are smaller, thinner and less robust than the heterozygotes at 5 weeks of age.

Heterozygous: Relatively normal.

 

Mammalian Phenotype Terms
Allelic Composition: Drd1tm1Stl/Drd1tm1Stl (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Drd1tm1Stl/Drd1tm1Stl (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Allelic Composition: Drd1tm1Stl/Drd1tm1Stl (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

MeSH Terms
  • Animals
  • Behavior, Animal/physiology
  • Cocaine/pharmacology
  • Dopamine/pharmacology
  • Electric Conductivity
  • Hyperkinesis/chemically induced
  • Male
  • Mice
  • Mice, Mutant Strains
  • Neurons/drug effects
  • Nucleus Accumbens/anatomy & histology
  • Nucleus Accumbens/physiology
  • Receptors, Dopamine D1/agonists
  • Receptors, Dopamine D1/deficiency
  • Receptors, Dopamine D1/genetics
  • Receptors, Dopamine D2/agonists
  • Receptors, Dopamine D2/metabolism
  • Signal Transduction/physiology
  • Spiperone/metabolism
  • Analysis of Variance
  • Base Sequence
  • Benzazepines/pharmacology
  • Brain/physiology
  • Catalepsy/chemically induced
  • Catalepsy/physiopathology
  • Chimera
  • Corpus Striatum/metabolism
  • Crosses, Genetic
  • DNA Primers
  • Dopamine/physiology
  • Dynorphins/biosynthesis
  • Female
  • Gene Expression
  • Habituation, Psychophysiologic
  • Immunohistochemistry
  • Interneurons/drug effects
  • Interneurons/physiology
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Motor Activity/drug effects
  • Neuronal Plasticity
  • Polymerase Chain Reaction
  • Pregnancy
  • Receptors, Dopamine D1/biosynthesis
Strain Development
E14 embryonic stem (ES) cells derived from 129P2/OlaHsd harboring the desired homologous recombination were identified. ES cells amplified from three of the homologous recombinants were used to generate male chimeric mice, and these were backcrossed onto C57BL/6 for three generations, then bred extensively with C57BL/6 females to obtain mice homozygous for D1 deletion.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Neurobiology
  • Obesity
Donor
Ming Xu, Ph.D., The University of Chicago.
Primary Reference
  • Xu M, Moratalla R, Gold LH, Hiroi N, Koob GF, Graybiel AM and Tonegawa S. Dopamine D1 receptor mutant mice are deficient in striatal expression of dynorphin and in dopamine-mediated behavioral responses. Cell. 1994 Nov 18;79(4):729-42. (Medline PMID:7954836)
  • Xu M, Hu XT, Cooper DC, Moratalla R, Graybiel AM, White FJ and Tonegawa S. Elimination of cocaine-induced hyperactivity and dopamine-mediated neurophysiological effects in dopamine D1 receptor mutant mice. Cell. 1994 Dec 16;79(6):945-55. (Medline PMID: 8001143)

Colony and Husbandry Information

The donor recommends providing food softened with water.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Agouti
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Backcross: 
    • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
  • Sib-mating: confirm there are no reproduction issues
    • Wild-type female x Heterozygous male littermate or reciprocal mating
    • Homozygous female x Homozygous male littermate
Generation
N3
Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced some pre-wean loss
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 months 4 months
Average litter size
4-6
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037113-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037113-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.