Strain Name:
B6;129-Fostm1Mxu/Mmjax
Stock Number:
037115-JAX
Citation ID:
RRID:MMRRC_037115-JAX
Other Names:
dopamine D1 receptor neuron-specific mutant mice, DC mice

Gene Information

Fostm1Mxu
Name: FBJ osteosarcoma oncogene; targeted mutation 1, Ming Xu
Synonyms: f/fc-fos
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Targeted Mutation
Fos
Name: FBJ osteosarcoma oncogene
Synonyms: c-fos, D12Rfj1, cFos
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Targeted Mutation
NCBI: 14281
HGNC: HGNC:3796
Homologene: 3844
Genetic Alterations

A region containing sequence encoding the DNA-binding and leucine zipper domains and a downstream neo transgene was flanked by single loxP sites. In situ hybridization and immunostaining indicated that the loxP sites did not interfere with transcription or translation.

Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype

Homozygous: Resistant to extinction of cocaine seeking.

Heterozygous: No observable phenotype

Strain Development
ES cells amplified from the homologous recombinants were injected into blastocysts isolated from C57BL/6 female mice. Then, the injected blastocysts were implanted back into (C57BL/6 x DBA/2)F1 females. The resulting male chimeric mice were repeatedly bred with C57BL/6 females. Mice were bred to homozygosity by crossing heterozygous mice. Upon arrival at MMRRC, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
Donor
Ming Xu, Ph.D., The University of Chicago.

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Agouti
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Homozygous female x Homozygous male littermate
Generation
F2
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037115-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037115-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.