Strain Name:
B6.129-Chst10tm1Mifu/Mmjax
Stock Number:
037129-JAX
Citation ID:
RRID:MMRRC_037129-JAX
Other Names:
HNK-1 ST KO

Gene Information

Chst10tm1Mifu
Name: carbohydrate sulfotransferase 10; targeted mutation 1, Minoru Fukuda
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Chst10
Name: carbohydrate sulfotransferase 10
Synonyms: ST, Hnk-1st
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 98388
Homologene: 21013
Genetic Alterations
A neo cassette replaced the coding region of Chst10 exon 5.
Genotype Determination
ES Cell Line
Not specified
Phenotype

Homozygous: Female mice have reduced fertility, decreased litter size, enlarged uterus in pro-estrus mice (increased uterus weight), which represents endometrium hyperplasia in pro-estrus mice. These results are consistent with increased circulating estrogen level in pro-estrus mice, reflecting lack of Chst10 gene increases unmodified estrogens. Male mice have reduced fertility and decreased litter size. Mechanism is not investigated further. Since it was found that Chst10 also modify glucuronidated-testosterone and DHEA, it is possible that development or maintenance of male reproductive system is perturbed in Chst10 knockout mice.

Heterozygous: Similar to homozygous. Circulating estrogen level in pro-estrus female mice seemed reduced (preliminary data). Male mice are not investigated well, but they have reduced feritility after back crossing.

Mammalian Phenotype Terms
Allelic Composition: Chst10tm1Mifu/Chst10tm1Mifu (Genetic Background: involves: 129 )

MeSH Terms
  • Animals
  • Estrogens/blood
  • Female
  • Gene Expression Regulation
  • Genetic Vectors
  • Glucuronic Acid/chemistry
  • Glycolipids/metabolism
  • HEK293 Cells
  • Humans
  • Killer Cells, Natural/cytology
  • Mice
  • Mice, Transgenic
  • Models, Genetic
  • Neurons/metabolism
  • Recombination, Genetic
  • Steroids/metabolism
  • Sulfotransferases/genetics
  • Sulfotransferases/metabolism
  • Testosterone/blood
Strain Development
Correctly targeted ES cells derived from 129/SvJ were injected into blastocysts to generate mutant mice. Heterozygous males were backcrossed to C57BL/6 wild-type female mice. However, three times backcrossed offspring no longer produced pups. Therefore, we had to rescue the line by in-vitro fertilization. IVF pups were fertile for the next seven backcrossings. Then the number of pups was reduced to 2-3 until eventually they stop producing again leaving the line to be rescued by IVF.
Suggested Control Mice
Wild-type littermates or C57BL/6 mice
  • Cancer
  • Endocrine Deficiency
  • Neurobiology
  • Reproduction
Donor
Minoru Fukuda, Ph.D., Sanford-Burnham Medical Research Institute.
Primary Reference

Suzuki-Anekoji M, Suzuki A, Wu SW, Angata K, Murai KK, Sugihara K, Akama TO, Khoo KH, Nakayama J, Fukuda MN, Fukuda M. In vivo regulation of steroid hormones by the Chst10 sulfotransferase in mouse. J Biol Chem. 2013 Feb 15;288(7):5007-16. Epub 2012 Dec 26. (Medline PMID: 23269668)

Colony and Husbandry Information

Though not required, inbreeding or crossing to a hybrid of C57BL/6 and 129/SvJ may allow line to keep breeding longer.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Backcross or IVF
Breeding Scheme(s)
  • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
Generation
N7
Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Reduced Reduced
Heterozygotes are fertile: Reduced Reduced
Age Reproductive Decline: 6 months 8 months
Average litter size
4-6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037129-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
037129-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.