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Strain Name:
NOD.Cg-Cd28tm1Mak H2h4/HbmMmmh
Stock Number:
037138-MU
Citation ID:
RRID:MMRRC_037138-MU
Other Names:
CD28-/- WT
Major Collection:

Strain Information

H2h4
Name: histocompatibility-2, MHC; h4 variant
Synonyms: H-2h4
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Non-mutant
H2
Name: histocompatibility-2, MHC
Synonyms: H-2, MHC-II
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Non-mutant
Cd28tm1Mak
Name: CD28 antigen; targeted mutation 1, Tak Mak
Synonyms: CD28KO, CD28-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
Cd28
Name: CD28 antigen
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Targeted Mutation
NCBI: 12487
HGNC: HGNC:1653
Homologene: 4473
Genotype Determination
ES Cell Line
Not applicable
Phenotype
SAT, Sjogren
Mammalian Phenotype Terms
Allelic Composition: H2h4/H2h4 (Genetic Background: NOD.Cg-H2h4 )

Allelic Composition: H2h4/H2g7 (Genetic Background: NOD.Cg-H2h4 )

Allelic Composition: Cd28tm1Mak/Cd28tm1Mak (Genetic Background: B6.129S2-Cd28tm1Mak )

Allelic Composition: Cd28tm1Mak/Cd28tm1Mak (Genetic Background: B6.129S2-Cd28tm1Mak/J )

Allelic Composition: Cd28tm1Mak/Cd28tm1Mak (Genetic Background: involves: 129S2/SvPas )

Allelic Composition: Cd28tm1Mak/Cd28tm1Mak (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

Allelic Composition: Cd28tm1Mak/Cd28tm1Mak (Genetic Background: involves: 129S2/SvPas * C57BL/6 * DBA/2 )

Allelic Composition: Cd28tm1Mak/Cd28tm1Mak (Genetic Background: involves: 129S2/SvPas * NOD )

Allelic Composition: Cd28tm1Mak/Cd28tm1Mak (Genetic Background: NOD.129S2-Cd28tm1Mak )

Allelic Composition: Cd28tm1Jmg/Cd28tm1Mak (Genetic Background: involves: 129S2/SvPas * 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Animals
  • Autoantibodies/immunology
  • B-Lymphocytes/immunology
  • CD28 Antigens/deficiency
  • CD4 Antigens/metabolism
  • Fibrosis/immunology
  • Forkhead Transcription Factors/metabolism
  • Inflammation
  • Interferon-gamma/biosynthesis
  • Interleukin-6/biosynthesis
  • Lymphocyte Count
  • Mice
  • Mice, Inbred NOD
  • Mice, Knockout
  • Receptors, Tumor Necrosis Factor, Type II/metabolism
  • Sodium Iodide/administration & dosage
  • T-Lymphocytes, Regulatory/immunology
  • Thyroglobulin/immunology
  • Thyroid Gland/immunology
  • Thyroiditis, Autoimmune/chemically induced
  • Thyroiditis, Autoimmune/immunology
  • Thyroxine/blood
  • Tumor Necrosis Factor Receptor Superfamily, Member 7/metabolism
Strain Development
The NOD.Cg-Cd28tm1Mak H2h4/Hbm was generated by crossing NOD.129S2(B6)-Cd28tm1Mak/JbsJ males with NOD.Cg-H2h4/DilTacUmmJ females then crossing F1 offspring to yield F2 mice. These were selected for absence of the NOD MHC and presence of the NOD.H-2h4 MHC (K haplotype) and presence of the homozygous mutant.
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Helen Braley-Mullen, Ph.D., University of Missouri.
Primary Reference

Ellis JS, Hong SH, Zaghouani H, Braley-Mullen H. Reduced effectiveness of CD4+Foxp3+ regulatory T cells in CD28-deficient NOD. H-2h4 mice leads to increased severity of spontaneous autoimmune thyroiditis. J Immunol. 2013 Nov 15;191(10):4940-9. Epub 2013 Oct 4. (Medline PMID: 24098053)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037138-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037138-MU-RESUS Litter recovered from cryo-archive $2,028.00 / $4,127.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.