Strain Name:
129S6;129S7-Gnao1tm1Lbi/Mmjax
Stock Number:
037339-JAX
Citation ID:
RRID:MMRRC_037339-JAX
Other Names:
Galpha-o KO

Gene Information

Gnao1tm1Lbi
Name: guanine nucleotide binding protein, alpha O; targeted mutation 1, Lutz Birnbaumer
Synonyms: Gnao1.1tm1LBi, Go1alpha
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Gnao1
Name: guanine nucleotide binding protein, alpha O
Synonyms: Go alpha, alphaO, Galphao
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
NCBI: 14681
HGNC: HGNC:4389
Homologene: 39203
Genetic Alterations
The PolII-neomycin cassette was inserted into the BamHI site of the Gnao1 gene in exon 6.
Genotype Determination
ES Cell Line
AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2
Phenotype

Homozygous: The homozygotes develop a turning phenotype and females do not reproduce. Undetermined if males are sterile.

Heterozygous: No observable phenotype.

Mammalian Phenotype Terms
Allelic Composition: Gnao1tm1Lbi/Gnao1tm1Lbi (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J )

MeSH Terms
  • Abnormalities, Multiple
  • Adenylyl Cyclases/metabolism
  • Animals
  • Behavior, Animal
  • Calcium/metabolism
  • Dimerization
  • Electric Conductivity
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • GTP-Binding Proteins/deficiency
  • GTP-Binding Proteins/genetics
  • Ganglia, Spinal/cytology
  • Ganglia, Spinal/physiology
  • Hyperalgesia
  • Mice
  • Mice, Mutant Strains
  • Motor Activity
  • Myocardium/enzymology
  • Nervous System Malformations
  • Neurons/physiology
Strain Development
The modified vector was transfected by electroporation into AB2.2 embryonic stem cells (129S7/SvEvBrd-Hprtb-m2). Correctly targeted ES cells were injected into C57BL/6 blastocysts, and the chimeric were bred to 129S6/SvEvTac to achieve an F1 generation heterozygous for Gnao1 loss. The heterozygotes were then bred to homozygosity for the loss of Gnao1. Homozygotes do not reproduce; the colony must be maintained as heterozygotes.
Suggested Control Mice
Wild-type littermates or 129S6/SvEvTac mice
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Metabolism
  • Neurobiology
  • Sensorineural
Donor
Lutz Birnbaumer, Ph.D., National Institute of Environmental Health Sciences.
Primary Reference

Jiang M, Gold MS, Boulay G, Spicher K, Peyton M, Brabet P, Srinivasan Y, Rudolph U, Ellison G, Birnbaumer L. Multiple neurological abnormalities in mice deficient in the G protein Go. Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3269-74. (Medline PMID: 9501252)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)

Wild-type (129S6/SvEvTac) female x Heterozygous male or reciprocal mating to refresh the colony

Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3 weeks
Average Pups Weaned
<4

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037339-JAX-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037339-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.