Strain Name:
STOCK Trpc1tm1Lbi/Mmjax
Stock Number:
037347-JAX
Citation ID:
RRID:MMRRC_037347-JAX
Other Names:
Trp1 KO

Gene Information

Trpc1tm1Lbi
Name: transient receptor potential cation channel, subfamily C, member 1; targeted mutation 1, Lutz Birnbaumer
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Trpc1
Name: transient receptor potential cation channel, subfamily C, member 1
Synonyms: Trrp1, Trp1, Mtrp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
NCBI: 22063
Homologene: 2478
Genetic Alterations
An EcoRI site in exon 8 was converted to a stop codon and a PGK-neo selection cassette replaced the terminal part of exon 8 along with intron 8. This resulted in loss of the coding region for amino acids 584 - 670 encoding transmembrane segments 4 and 5 and a small part of the putative pore domain. RT-PCR analysis detected a shorter transcript in which all of exon 8 was skipped resulting in a frameshift and stop codon at position 2 of exon 9.
Genotype Determination
ES Cell Line
CCE/EK.CCE derived from 129S8/SvEv-Gpi1c
Phenotype

Homozygous: Homozygous mice demonstrate an altered allergic immune response. 

Heterozygous: No observable phenotype.

Mammalian Phenotype Terms
Allelic Composition: Trpc1tm1Lbi/Trpc1tm1Lbi (Genetic Background: involves: 129S/SvEv )

Allelic Composition: Trpc1tm1Lbi/Trpc1tm1Lbi (Genetic Background: involves: 129S/SvEv * C57BL/6 )

Allelic Composition: Trpc1tm1Lbi/Trpc1tm1Lbi (Genetic Background: involves: 129S/SvEv * C57BL/6J )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Aorta, Thoracic/cytology
  • Base Sequence
  • Calcium Channels
  • Cerebral Arteries/cytology
  • Indoles/pharmacology
  • Inositol 1,4,5-Trisphosphate/pharmacology
  • Membrane Glycoproteins/antagonists & inhibitors
  • Membrane Glycoproteins/biosynthesis
  • Mice
  • Molecular Sequence Data
  • Muscle, Smooth, Vascular/cytology
  • Muscle, Smooth, Vascular/drug effects
  • Muscle, Smooth, Vascular/physiology
  • RNA, Small Interfering/pharmacology
  • Stromal Interaction Molecule 1
  • TRPC Cation Channels/deficiency
  • TRPC Cation Channels/physiology
  • Thapsigargin/pharmacology
Strain Development
The modified construct was electroporated into CCE/EK.CCE ES cells (129S8/SvEv-Gpi1c). Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeras were bred with 129S8/SvEv-Gpi1c to homozygosity. The line has been maintained by breeding homozygotes.

Note: A 32-SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at the MMRRC at JAX. Five of the 27 markers throughout the genome were segregating from an unknown source.
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Research Tools
Donor
Lutz Birnbaumer, Ph.D., National Institute of Environmental Health Sciences.
Primary Reference

Dietrich A, Kalwa H, Storch U, Mederos y Schnitzler M, Salanova B, Pinkenburg O, Dubrovska G, Essin K, Gollasch M, Birnbaumer L, Gudermann T. Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1. Pflugers Arch. 2007 Dec;455(3):465-77. (Medline PMID: 17647013)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)

Homozygous female x Homozygous male littermate

Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
4-6
Recommended wean age
3 weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037347-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037347-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.