Strain Name:
STOCK Trpc7tm1.1Lbi/Mmjax
Stock Number:
037348-JAX
Citation ID:
RRID:MMRRC_037348-JAX
Other Names:
Trpc7tm1.1Kofu

Gene Information

Trpc7tm1.1Lbi
Name: transient receptor potential cation channel, subfamily C, member 7; targeted mutation 1.1, Lutz Birnbaumer
Synonyms: Trpc7tm1.1Kofu
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
Trpc7
Name: transient receptor potential cation channel, subfamily C, member 7
Synonyms: TRP7, Trrp8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
NCBI: 26946
Homologene: 22689
Genetic Alterations
A floxed neo cassette was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 5. Cre-mediated recombination removed the neo cassette and exon 5.
Genotype Determination
ES Cell Line
Not specified
Phenotype

Homozygous: No observable phenotype.

Heterozygous: No observable phenotype.

Mammalian Phenotype Terms
Allelic Composition: Trpc7tm1.1Lbi/Trpc7tm1.1Lbi (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA )

MeSH Terms
  • Animals
  • Diglycerides/metabolism
  • Humans
  • Light
  • Light Signal Transduction/physiology
  • Mice
  • Mice, Knockout
  • Patch-Clamp Techniques
  • Photic Stimulation
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Protein Subunits/genetics
  • Protein Subunits/metabolism
  • Retinal Ganglion Cells/cytology
  • Retinal Ganglion Cells/physiology
  • Rod Opsins/metabolism
  • TRPC Cation Channels/genetics
  • TRPC Cation Channels/metabolism
Strain Development

The targeting vector contained loxP sites flanking exon 5 and the PGK-neo cassette. In ES cells, loxP recombination sites were introduced by homologous recombination into the introns bordering exon 5 of the murine Trpc7 gene. Correctly-targeted ES cells (129S6/SvEvTac) were implanted into blastocysts and chimeric mice were interbred to generate mice homozygous for the Trpc7 gene with floxed exon 5 (Trpc7x/x). Male Trpc7 x/x mice were crossed to female mice carrying a transgene directing the expression of Cre recombinase under the control of the Sox2 promoter (B6.Cg-Tg(Sox2-cre)1Amc/J, JAX Strain #008454). Heterozygous null-mice/Sox2-Cre transgene-negative were bred to homozygosity.

Suggested Control Mice
Wild-type littermates
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Neurobiology
  • Research Tools
Donor
Lutz Birnbaumer, Ph.D., National Institute of Environmental Health Sciences.
Primary Reference

Perez-Leighton CE, Schmidt TM, Abramowitz J, Birnbaumer L, Kofuji P. Intrinsic phototransduction persists in melanopsin-expressing ganglion cells lacking diacylglycerol-sensitive TRPC subunits. Eur J Neurosci. 2011 Mar;33(5):856-67. (Medline PMID: 21261756)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)

Homozygous female x Homozygous male littermate

Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
4-6
Recommended wean age
3 weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037348-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037348-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.