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Strain Name:
NOD.Cg-Ighmtm1Cgn H2h4 Tg(Foxp3-HBEGF/EGFP)1Doi/HbmMmmh
Stock Number:
037354-MU
Citation ID:
RRID:MMRRC_037354-MU
Major Collection:

Strain Information

Ighmtm1Cgn
Name: immunoglobulin heavy constant mu; targeted mutation 1, University of Cologne
Synonyms: muMt-, Ig-, mu-MT-, IgHmuMT, BKO, Ighm-, Igmunull, Cmu -, Ig- muMT, B cell-, Igh-6tm1Cgn, muIgKO, muMT, BCKO, Igh-6null, Igh-6-, Igh-6tm1CgnmuMT0, mum-, IgH-, BCR-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Ifngtm1Ts
Name: interferon gamma; targeted mutation 1, Timothy Stewart
Synonyms: IFN-gamma KO, Ifngtm1Ts, gko, ifntm1Ts, ifgtm1, Ifngtm1Ts, IFN-g KO, Ifntm/Ts, GKO, IFN-gammatm1Ts, ifng-, IFNgamma KO, IFN-gammaKO, IFN-gamma-, Ifn-gtm1Ts, IFNgamma-, IFN-gamma KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Ighm
Name: immunoglobulin heavy constant mu
Synonyms: Ig mu, Igh6, Igh-M, muH, IgM
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knockout
NCBI: 16019
HGNC: HGNC:5541
Ifng
Name: interferon gamma
Synonyms: IFN-gamma, Ifg
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knockout
NCBI: 15978
VEGA: 10
HGNC: HGNC:5438
Homologene: 55526
H2h4
Name: histocompatibility-2, MHC; h4 variant
Synonyms: H-2h4
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Non-mutant
H2
Name: histocompatibility-2, MHC
Synonyms: H-2, MHC-II
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Non-mutant
Tg(Foxp3-HBEGF/EGFP)1Doi
Name: transgene insertion 1, Christophe Benoist
Synonyms: Foxp3DTR, Foxp3-DTR
Type: Allele
Species: Multi-species
Chromosome: unknown
Alteration at locus: Transgenic
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Alteration at locus: Transgenic
Foxp3
Name: forkhead box P3
Synonyms: scurfin, JM2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Transgenic
NCBI: 20371
HGNC: HGNC:6106
Homologene: 8516
HBEGF
Name: heparin-binding EGF-like growth factor
Type: Gene
Species: Homo sapiens (human)
Chromosome: 5
Alteration at locus: Transgenic
Genotype Determination
ES Cell Line
AB1 derived from 129S7/SvEvBrd-Hprt+
Mammalian Phenotype Terms
Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: B6.129S2-Ighmtm1Cgn )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: B6.129S2-Ighmtm1Cgn/J )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: involves: 129S2/SvPas )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: NOD.129S2-Ighmtm1Cgn )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: NOD.129S2-Ighmtm1Cgn/DvsJ )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: NODCaj.129S2-Ighmtm1Cgn )

Allelic Composition: Ighmtm1Cgn/Ighm+ (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

Allelic Composition: Ighmtm1Cgn/Ighm+ (Genetic Background: NOD.129S2-Ighmtm1Cgn )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: B6.129S7-Ifngtm1Ts )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: B6.129S7-Ifngtm1Ts/J )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: C.129S7(B6)-Ifngtm1Ts/J )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: C.129S7-Ifngtm1Ts )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * A/WySn * C57BL/10SnSg * Swiss )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * BALB/c )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * DBA/1 * MRL/Mp )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * NOD )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: NOD.129S7(B6)-Ifngtm1Ts Prkdcscid )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: NOD.129S7(B6)-Ifngtm1Ts/DvsJ )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: NOD.129S7-Ifngtm1Ts )

Allelic Composition: H2h4/H2h4 (Genetic Background: NOD.Cg-H2h4 )

Allelic Composition: H2h4/H2g7 (Genetic Background: NOD.Cg-H2h4 )

Strain Development
The NOD.Cg-Ifngtm1Ts Ighmtm1Cgn H2h4 Tg(Foxp3-HBEGF/EGFP)1Doi/Hbm line was generated by crossing NOD/ShiLtJ-Tg(Foxp3-HBEGF/EGFP)1Doi/J (JAX Strain #028763) males with NOD.Cg-Ifngtm1Ts Ighmtm1Cgn H2h4/Hbm (MMRRC:037144) females then crossing F1 offspring to yield F2 mice. These were selected for absence of the NOD MHC and presence of the NOD.H2h4 MHC (K haplotype) and presence of the homozygous mutant.
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Helen Braley-Mullen, Ph.D., University of Missouri.
Christophe Benoist, M.D., Harvard Medical School.
Klaus Rajewsky, Ph.D., Max-Delbrück-Centrum für Molekulare Medizin.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037354-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037354-MU-RESUS Litter recovered from cryo-archive $2,028.00 / $4,127.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.