Strain Name:
129.Cg-H2afy2tm1.1Peh H2afytm1Peh/Mmjax
Stock Number:
037356-JAX
Citation ID:
RRID:MMRRC_037356-JAX
Other Names:
macroH2A1/2 double knockout

Gene Information

Macroh2a1tm1Peh
Name: macroH2A.1 histone; targeted mutation 1, John R Pehrson
Synonyms: macroH2A1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
Macroh2a2tm1.1Peh
Name: macroH2A.2 histone; targeted mutation 1.1, John R Pehrson
Synonyms: macroH2A2 KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Macroh2a1
Name: macroH2A.1 histone
Synonyms: mH2a1, MACROH2A1.2, H2AF12M, H2afy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
NCBI: 26914
HGNC: HGNC:4740
Homologene: 3598
Macroh2a2
Name: macroH2A.2 histone
Synonyms: H2afy2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
NCBI: 404634
Homologene: 10246
Genetic Alterations
  • H2afytm1Peh: An IRESBgeo cassette was inserted in first intron. This cassette was flanked by loxP sites and a third loxP site was inserted in the second intron. Transient cre expression removed the floxed sequence, including exon 2, which encodes the first part of the H2A domain.
  • H2afy2tm1.1Peh: The Neo cassette was flanked by loxP recombination sites and inserted into an EaeI site located less than 300 bp upstream of exon 2. A third loxP site was inserted into an EaeI site located less than 150 bp downstream of exon 2. Cre excision was used to remove the floxed sequence, including exon 2.
ES Cell Line
  • H2afytm1Peh: Not specified derived from 129X1/SvJ
  • H2afy2tm1.1Peh: v6.5 derived from (C57BL/6 x 129S4/SvJae)F1
Phenotype

Homozygous: Late stage fetuses are smaller; postnatal growth is slower; young adult mice are shorter and have less lean mass; the eyelid margin appears to be wider; the coat color is darker than normal at 6 weeks of age .

Heterozygous: No observable phenotype.

Mammalian Phenotype Terms
Allelic Composition: H2afytm1Peh/H2afytm1Peh (Genetic Background: B6.129X1-H2afytm1Peh )

Allelic Composition: (Genetic Background: )

  • normal phenotype
MeSH Terms
  • Animals
  • Animals, Newborn
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Silencing
  • Glucose/metabolism
  • Histones/biosynthesis
  • Histones/genetics
  • Liver/metabolism
  • Mice
  • Mice, Knockout
  • Mutation
  • Nucleosomes/genetics
  • Nucleosomes/metabolism
  • X Chromosome/genetics
  • X Chromosome/metabolism
  • X Chromosome Inactivation/genetics
  • X Chromosome Inactivation/physiology
Strain Development
H2afytm1Peh: Correctly targeted ES cells derived from 129X1/SvJ were injected into C57BL/6 blastocysts, and the chimeric males were mated to obtain germ line transmission of the macroH2A1 knockout allele. The macroH2A1 knockout allele was backcrossed into the C57BL/6 background for 10 generations.
  • H2afy2tm1.1Peh: The targeting vector was electroporated into v6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1. ES cells positive for homologous recombination by southern blotting were injected into blastocysts and the chimeric males were bred to females to obtain germ line transmission. Recombinant mice were bred with FVB/N-TgN(EIIa-Cre)C5379Lmgd to remove the neo-cassette and exon 2 on the H2afy2 gene. The resulting knockout was backcrossed into the 129S6/SvEvTac background for 10 or more generations.
  • The double knockout was generated by mating the individual knockout strains. Offspring were bred together and maintained as sib mating. Fertility may decrease more rapidly than wild-type 129 mice, so the mice are typically bred when they are young, less than 4 months.
  • Suggested Control Mice
    Wild-type littermates
    • Developmental Biology
    • Metabolism
    • Research Tools
    Donor
    John Pehrson, Ph.D., University of Pennsylvania.
    Primary Reference

    Changolkar LN, Costanzi C, Leu NA, Chen D, McLaughlin KJ and Pehrson JR. Developmental changes in histone macroH2A1-mediated gene regulation. Mol Cell Biol. 2007 Apr;27(7):2758-64. Epub 2007 Jan 22. (Medline PMID:17242180)

    Colony and Husbandry Information

    Fertility may decrease more rapidly than wild-type 129 mice, so mice are typically bred when they are less than 4 months.
    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
    Coat Color
    Agouti
    Other
    Many double knockout become darker on their back than wild-type controls at around 6 weeks of age.
    MMRRC Breeding System
    Sib-mating
    Breeding Scheme(s)
    • Wild-type female x Heterozygous male littermate or reciprocal mating
    • Homozygous female x Homozygous male littermate
    Generation
    N10
    Overall Breeding Performance
    Good, per donating investigator: the fertility of the mutants may decrease more rapidly than wild-type 129 mice, so we typically breed them when they are young, less than 4 months.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Reduced Reduced Fertility may decrease more rapidly than wild-type 129 mice, so mice are typically bred when they are less than 4 months.
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4-6
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    037356-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
    037356-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.