Strain Name:
B6NTac.Cg-Tg(Lck-cre)1Cwi/Mmnc
Stock Number:
037396-UNC
Citation ID:
RRID:MMRRC_037396-UNC

Strain Information

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Lck
Name: lymphocyte protein tyrosine kinase
Synonyms: Hck-3, p56lck
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16818
HGNC: HGNC:6524
Homologene: 3911
Tg(Lck-cre)1Cwi
Name: transgene insertion 1, Christopher B Wilson
Synonyms: lckCre, iLckcre, pLck-cre, cwLckCre, Lck-Cre, lck-cre, Lck-Cre+, plck-cre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations
This transgene expresses Cre recombinase under the control of the Lck promoter, active in T cells during development.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Lck-Cre transgenic mouse uses the proximal promoter of the Lck (lymphocyte protein tyrosine kinase) gene, which is first expressed early in thymocyte development at the double negative stage. After T cells fully mature, the level of expression of this transgene decreases by approximately 10 fold. This particular mouse shows a high degree of expression of the transgene in the thymus
    MeSH Terms
    • Animals
    • Cell Differentiation/genetics
    • Cell Differentiation/immunology
    • Cell Lineage/genetics
    • Cell Lineage/immunology
    • DNA (Cytosine-5-)-Methyltransferase 1
    • DNA (Cytosine-5-)-Methyltransferases/genetics
    • DNA (Cytosine-5-)-Methyltransferases/immunology
    • DNA Methylation
    • Gene Expression Regulation/immunology
    • Mice
    • Mice, Transgenic
    • T-Lymphocytes/immunology
    Strain Development
    The Lck-Cre mice were created by pronuclear microinjection of this construct into B6DF2 fertilized eggs. These mice were first backcrossed three times to B6 and then bred to produce homozygotes. Later they were bred to mice of a mixed B6;129Sv/J background. The resultant STOCK-Tg(Lck-Cre)1Cwi mice were screened for H-2 expression and offspring homozygous for H-2b were selected for breeding. Homozygous Lck-Cre mice maintained on this mixed background were then transferred to the NIAID repository at Taconic for embryo rederivation by crossing with C57BL/6NTac mice. These pups were then backcrossed to C57BL/6NTac for a total of 9 generations. Those carrying the transgene were further intracrossed to homozygosity.
    Suggested Control Mice
    Wild-type or C57BL/6NTac mice
    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on one XY samples (QZ1372) at the time of importation and the results of the analysis are congruent with the SDS. The background is C57BL/6NTac and there is no evidence of a secondary background. The mitochondrial genome and Y chromosome are consistent with C57BL/6NTac. The Cre construct is detected in the sample.

    The genome of the MMRRC: 37396 strain is 100 % inbred and 100% congenic at the strain/substrain level which is consistent with the number of backcross generations reported in the SDS.
    MiniMUGA Sample Report
    • Immunology and Inflammation
    • This mouse shows a high degree of expression of the transgene in the thymus and has been found to bring about the selective deletion of genes flanked by loxP targeting sequences in almost all early thymocytes. It thus will be useful for experiments designed to delete a specific gene in the T cell lineage starting at the double negative stage.
      Donor
      Chris Wilson, Ph.D., University of Washington
      Primary Reference
      Lee PP, Fitzpatrick DR, Beard C, Jessup HK, Lehar S, Makar KW, Perez-Melgosa M, Sweetser MT, Schlissel MS, Nguyen S, Cherry SR, Tsai JH, Tucker SM, Weaver WM, Kelso A, Jaenisch R, Wilson CB. A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival. Immunity. 2001 Nov;15(5):763-74. (Medline PMID: 11728338)

      Colony and Husbandry Information

      Colony Surveillance Program and Current Health Reports

      Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
      Coat Color
      Black
      MMRRC Breeding System
      Intra-strain Random Mating
      Breeding Scheme(s)
      • Homozygous female x Homozygous male from the colony
      Generation
      N9
      Overall Breeding Performance
      Not specified
      NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
      Viability and Fertility: Female Male Comments
      Homozygotes are viable: Yes Yes
      Homozygotes are fertile: Yes Yes
      Hetero/Hemizygotes are fertile: Yes Yes
      Age Reproductive Decline: Not specified Not specified
      Average litter size
      Not specified
      Recommended wean age
      3 weeks

      Order Request Information

      Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

      Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

      Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

      The donor or their institution limits the distribution to non-profit institutions only.

      Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

      Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
      MMRRC Item # Description Distribution Fee / Unit (US $)
      *Shipping & Handling not included*
      Units Notes
      037396-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
      037396-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
      037396-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

      1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

      2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

      3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

      4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

      To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.