Strain Name:
B6J.129P2-H2-D1b-tm1Bpe/TacMmucd
Stock Number:
037401-UCD
Citation ID:
RRID:MMRRC_037401-UCD

Strain Information

H2-D1b-tm1Bpe
Name: histocompatibility 2, D region locus 1; targeted mutation 1, Beatrice Perarnau
Synonyms: HHD, H-2 Dbo, D-, H-2Do, H-2Db knockout, H2-D1tm1Bpe, H-2Db-, Db-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
H2-D1
Name: histocompatibility 2, D region locus 1
Synonyms: H-2D
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Knockout
NCBI: 14964
Homologene: 128352
Genetic Alterations
Exons 1, 2 and 3 of the b haplotype gene were replaced with plasmid sequences and a neomycin cassette.
Genotype Determination
ES Cell Line
CGR8
Phenotype
H-2 class I molecules are trimeric complexes assembled in the ER and formed by the association of a 42-kDa heavy chain, b2 microglobulin and a short (8-11 AA) peptide. Although there is a profound reduction of H-2 Class I molecules in the b2m-targeted mutation mouse, there is still residual expression of functional H2-Db molecules. In contrast, no H2-Db molecules can be detected by indirect immunofluorescence analysis of peripheral T lymphocytes from the H2-Db-/- mouse. A compensatory increase in cell surface expression of other class I molecules was observed resulting in almost unaltered levels of cell surface expression of b2m. This increase cannot be attributed solely to enhanced expression of Q7 or Q9 (the most abundant Class Ib molecules at the surface of T lymphocytes) or to H2-Kb implying that there may be increased cell surface expression of other, unidentified class Ib molecules. This mouse model is a useful tool for evaluating the immunological potential of individual MHC class I molecules and for studying cytolytic T cell education as well as the role of Class Ib molecules.
MeSH Terms
  • Animals
  • Cytotoxicity, Immunologic
  • H-2 Antigens/physiology
  • HLA-A2 Antigen/physiology
  • Histocompatibility Antigen H-2D
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • T-Lymphocytes, Cytotoxic/immunology
  • beta 2-Microglobulin/physiology
  • CD8-Positive T-Lymphocytes/immunology
  • Cell Line
  • H-2 Antigens/genetics
  • Lymphocytic Choriomeningitis/immunology
  • Lymphocytic choriomeningitis virus/immunology
Strain Development
C57BL/6 mice deficient in Db were generated by homologous recombination in the laboratory of Dr. Francois Lemonnier of The Institut Pasteur, Paris France.

Generation of H2-Db knockout mice: The H2-Db gene was disrupted using an insert containing LacZ and a neo cassette flanked by a 4-kb HindIII-XbaI 5' and 1-kb KpnI-SpeI 3' fragment of the Db gene resulting in the loss of the first three exons. The targeting construct was introduced into a CGR-8 embryonic stem cell of 129P2/Ola origin. Clones that were confirmed for homologous recombination were injected into B6 blastocysts and germline chimeras were identified. The founders were crossed twice with C57BL/6J females and heterozygous N2 offspring were intercrossed to generate the H2-Db homozygous strain. Inactivation of H2-Db was confirmed by Southern blot analysis on tail DNA and immunofluorescence assay. Subsequently, the N2 mice were backcrossed 10 more times to C57BL/6J and then intercrossed to produce N12 homozygous H2-Db-/- targeted mutation mice. Six pairs of the N12 homozygous mice were transferred from Dr. Lemonnier to the NIAID repository at Taconic in 2001 where they were embryo transfer-derived. Subsequent transfer to the MMRRC.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
Donor
Marion Berard, D.V.M., Institut Pasteur.
Primary Reference

Pascolo S, Bervas N, Ure JM, Smith AG, Lemonnier FA, Pérarnau B.HLA-A2.1-restricted education and cytolytic activity of CD8(+) T lymphocytes frombeta2 microglobulin (beta2m) HLA-A2.1 monochain transgenic H-2Db beta2m doubleknockout mice. J Exp Med. 1997 Jun 16;185(12):2043-51. (Medline PMID: 9182675)

Pérarnau B, Saron MF, Reina San Martin B, Bervas N, Ong H, Soloski MJ, SmithAG, Ure JM, Gairin JE, Lemonnier FA. Single H2Kb, H2Db and double H2KbDb knockoutmice: peripheral CD8+ T cell repertoire and anti-lymphocytic choriomeningitisvirus cytolytic responses. Eur J Immunol. 1999 Apr;29(4):1243-52. (Medline PMID: 10229092)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross then sib-mating
Generation
N12 (C57BL/6)

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037401-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037401-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
037401-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.