Strain Name:
B6;129P2-Tg(CAG-BGeo,-tTA,-EGFP)2A11Kuw/Mmmh
Stock Number:
037406-MU
Citation ID:
RRID:MMRRC_037406-MU
Other Names:
CAG-LSL-tTA

Gene Information

CAG
Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor
Type: DNA Segment
Species: Multi-species
tTA
Name: tetracycline-controlled transactivator protein
Type: Gene
Species: Multi-species
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Bgeo
Name: beta-galactosidase
Type: Gene
Species: Escherichia coli (bacterium)
Tg(CAG-Bgeo,-tTA,-EGFP)2A11Kuw
Name: transgene insertion 2A11, Kay-Uwe Wagner
Synonyms: CAG-loxP-betageo-loxP-tTA-IRES-GFP, CAG-bGeo-tTA-IRES-GFP, pCCALL2-tTA-IRES-GFP, CAG-betageo-tTA, CAG-tTA-IRES-GFP
Type: Transgene
Species: Mus musculus (mouse)
Alteration at locus: Transgenic
Genetic Alterations
This transgene contains the CAG promoter, consisting of the CMV-IE enhancer followed by the chicken beta-actin (ACTB) upstream promoter, first exon and first intron, driving expression of a loxP-flanked βgeo gene with three copies of the SV40 polyadenylation signal. Upon excision of βgeo by Cre recombinase, the tetracycline transactivator (tTA) and enhanced green fluorescent protein (EGFP) genes are expressed from the CAG promoter. Presence of the tTA protein promotes expression of other transgenes regulated by the tet operon (tetO); this expression is readily suppressed by doxycycline.
Genotype Determination
ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype

Homozygous: No observable phenotype. 

Hemizygous: No observable phenotype. 

Expected Phenotype After Cre-excision: Expression of the tTA, which is strictly dependent on the presence of Cre recombinase, induces the activation of the reporter transgene in the absence of any ligands. The tTA-mediated transactivation can be completely ablated through administration of doxycycline, and its subsequent withdrawal lifts the transcriptional block. Permits a temporally and spatially controlled expression of responder genes in embryonic and multiple adult tissues. 

MeSH Terms
  • Actins/genetics
  • Animals
  • Blotting, Southern
  • Cell Line
  • Cloning, Molecular
  • Cytomegalovirus/genetics
  • DNA Primers/genetics
  • Embryo, Mammalian/metabolism
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Enhancer Elements, Genetic/genetics
  • Galactosides
  • Gene Expression Regulation/genetics
  • Genotype
  • Humans
  • Indoles
  • Luciferases
  • Mice
  • Mice, Transgenic/genetics
  • Organ Specificity
  • Promoter Regions, Genetic/genetics
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • Transgenes/genetics
  • Transgenes/physiology
  • Cell Transformation, Neoplastic/genetics
  • Disease Models, Animal
  • Flow Cytometry
  • Genes, myc
  • Genes, p16
  • Neoplasm Recurrence, Local
  • Pancreatic Neoplasms/genetics
  • Pancreatic Neoplasms/pathology
Strain Development

The CAG-βgeo-tTA vector was linearized using NotI, phenol-chlorophorm, extracted, and electroporated into E14 (129P2/OlaHsd) embryonic stem cells. After selecting neomycin-resistant ES cells. with uniform and intense lacZ expression, ES cells were injected into C57BL/6 blastocysts and chimeric males were mated with C57BL/6 females. The transgenic line was crossed C57BL/6 and maintained with sib-mating.

Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cancer
  • Developmental Biology
  • Diabetes
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Research Tools
Donor
Kay-Uwe Wagner, Ph.D., University of Nebraska Medical Center.
Primary Reference
  • Zhang Q, Triplett AA, Harms DW, Lin WC, Creamer BA, Rizzino A, Wagner KU. Temporally and spatially controlled expression of transgenes in embryonic and adult tissues. Transgenic Res. 2010 Jun;19(3):499-509. (Medline PMID: 19821046)
  • Lin WC, Rajbhandari N, Liu C, Sakamoto K, Zhang Q, Triplett AA, Batra SK, Opavsky R, Felsher DW, DiMaio DJ, Hollingsworth MA, Morris JP 4th, Hebrok M, Witkiewicz AK, Brody JR, Rui H, Wagner KU. Dormant cancer cells contribute to residual disease in a model of reversible pancreatic cancer. Cancer Res. 2013 Mar 15;73(6):1821-30. (Medline PMID: 23467612)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Backcross
    • Wild-type (C57BL/6) female x Hemizygous male or reciprocal mating
  • Sib-mating
    • Wild-type female x Hemizygous male littermate or reciprocal mating
Generation
N2
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037406-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037406-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.