Strain Name:
B6.Cg-Bcl2a1atm1Prys/Mmjax
Stock Number:
037468-JAX
Citation ID:
RRID:MMRRC_037468-JAX
Other Names:
B6-A1aKO

Gene Information

Bcl2a1atm1Prys
Name: B cell leukemia/lymphoma 2 related protein A1a; targeted mutation 1, Michael Prystowsky
Synonyms: Bcl2a1atm1Orlf, A1-a-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Bcl2a1a
Name: B cell leukemia/lymphoma 2 related protein A1a
Synonyms: A1, Bfl-1, Bcl2a1, Hbpa1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
NCBI: 12044
HGNC: HGNC:991
Homologene: 2988
Genetic Alterations
A region of the Bcl2a1a gene, including 386 bp of 5' flank, exon 1, and part of intron 1, has been replaced by a neomycin resistance gene.
ES Cell Line
WW6 derived from STOCK 129/Sv and C57BL/6J and SJL
Phenotype

Homozygous: Reduced acute inflammatory response to Toxoplasma gondii. Neutropenia in the C57BL/6J background.

Heterozygous: Mild neutropenia in the C57BL/6J background.

 

Mammalian Phenotype Terms
Allelic Composition: Bcl2a1atm1Prys/Bcl2a1atm1Prys (Genetic Background: involves: 129/Sv * C57BL/6J * SJL )

MeSH Terms
  • Acute Disease
  • Animals
  • Apoptosis
  • Cells, Cultured
  • Female
  • Gene Targeting
  • In Situ Nick-End Labeling
  • Inflammation/immunology
  • Inflammation/parasitology
  • Kinetics
  • Leukocytes, Mononuclear/ultrastructure
  • Leukocytosis/immunology
  • Leukocytosis/parasitology
  • Macrophages/immunology
  • Macrophages/parasitology
  • Macrophages/ultrastructure
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Minor Histocompatibility Antigens
  • Peritonitis/immunology
  • Peritonitis/parasitology
  • Proto-Oncogene Proteins c-bcl-2/genetics
  • Proto-Oncogene Proteins c-bcl-2/physiology
  • RNA, Messenger/biosynthesis
  • Toxoplasma/growth & development
  • Toxoplasmosis, Animal/immunology
  • Toxoplasmosis, Animal/parasitology
  • Up-Regulation
Strain Development
Correctly targeted WW6 ES cells derived from a STOCK mix of 129/Sv, C57BL/6J, and SJL, were injected into C57BL/6 blastocysts. The resulting chimeras were mated with C57BL/6 mice. Heterozygous progeny were backcrossed for 10 generations to C57BL/6J and then random intra-strain mating to generate homozygous progeny.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
  • Apoptosis
  • Cancer
  • Cell Biology
  • Hematology
  • Immunology and Inflammation
Donor
Amos Orlofsky, Ph.D., Albert Einstein College of Medicine.
Primary Reference

Orlofsky A, Weiss LM, Kawachi N, Prystowsky MB. Deficiency in the Anti-Apoptotic Protein A1-a Results in a Diminished Acute Inflammatory Response. J Immunol. 2002 Feb 15;168(4):1840-6. (Medline PMID:11823517)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Backcross or Random intra-strain mating
Breeding Scheme(s)
  • Backcross:
    • Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating
  • Intra-strain Random Mating
    • Wild-type female x Heterozygous male from the colony or reciprocal mating
    • Homozygous female x Homozygous male from the colony
Generation
N10
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037468-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037468-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.