Strain Name:
B6.129S4(Cg)-H2afy2tm1.1Peh/Mmjax
Stock Number:
037474-JAX
Citation ID:
RRID:MMRRC_037474-JAX
Other Names:
macroH2A2

Gene Information

Macroh2a2tm1.1Peh
Name: macroH2A.2 histone; targeted mutation 1.1, John R Pehrson
Synonyms: macroH2A2 KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Macroh2a2
Name: macroH2A.2 histone
Synonyms: H2afy2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
NCBI: 404634
Homologene: 10246
Genetic Alterations
An IRESBgeo cassette was inserted in 1st intron. This cassette was flanked by loxP sites and a 3rd loxP site was inserted in the 2nd intron. Cross with an EIIa-cre mouse removed the floxed sequence, including exon 2.
ES Cell Line
v6.5 derived from (C57BL/6 x 129S4/SvJae)F1
Phenotype

Homozygous: No observable phenotype

Heterozygous: No observable phenotype

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

  • normal phenotype
Strain Development
The targeting vector was electroporated into v6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1. ES cells positive for homologous recombination by southern blotting were injected into blastocysts and the chimeric males were bred to females to obtain germ line transmission. Recombinant mice were bred with FVB/N-TgN(EIIa-Cre)C5379Lmgd to remove the neo-cassette and exon 2 on the H2afy2 gene. The resulting knockout was backcrossed into the C57BL/6 background for 10 or more generations. Line has been maintained as sib-mating.
Suggested Control Mice
Wild-type littermates or C57BL/6 mice
  • Cancer
  • Cell Biology
  • Metabolism
Donor
John Pehrson, Ph.D., University of Pennsylvania.

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Backcross:
    • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
  • Sib-mating:
    • Wild-type female x Heterozygous male littermate or reciprocal mating
    • Homozygous female x Homozygous male littermate
Generation
N10
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037474-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037474-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.