Strain Name:
FVB/N-Tg(Isl1-lacZ)5Cka/Mmjax
Stock Number:
037490-JAX
Citation ID:
RRID:MMRRC_037490-JAX
Other Names:
3cF10-5

Gene Information

Isl1
Name: ISL1 transcription factor, LIM/homeodomain
Synonyms: Islet 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16392
HGNC: HGNC:6132
Homologene: 1661
Tg(Isl1-lacZ)5Cka
Name: transgene insertion 5, Claudia Kappen
Synonyms: 3cF10
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: UN
Alteration at locus: Transgenic
Genetic Alterations
Insertion of a transgene in which LacZ expression is dependent on regulatory elements from the Isl1 locus.
Phenotype

Homozygous: Viable

Hemizygous: No observable phenotype

MeSH Terms
  • Animals
  • Cell Line
  • Enhancer Elements, Genetic
  • Female
  • Fetal Heart/embryology
  • Fetal Heart/metabolism
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors/metabolism
  • GATA4 Transcription Factor/metabolism
  • Gene Expression Regulation, Developmental
  • Gene Library
  • Genes, Reporter
  • Homeodomain Proteins/genetics
  • LIM-Homeodomain Proteins
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Mice
  • Mice, Transgenic
  • Pancreas/metabolism
  • Pregnancy
  • Regulatory Elements, Transcriptional
  • Transcription Factors
  • Transfection
Strain Development
The transgene was injected into fertilized FVB/N oocytes. Founder mouse bred to FVB/N wild-type mice. Line has been maintained as random intra-strain mating.
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Developmental Biology
  • Diabetes
  • Models for Human Disease
    • Diabetic pregnancy
    • Diabetic embryopathy
Donor
Claudia Kappen, Ph.D., Pennington Biomedical Research Center.
Primary Reference

Kappen C, Salbaum JM. Identification of gene regulatory elements in the Islet-1 gene locus. Int J Dev Biol. 2009;53(7):935-46. (Medline PMID: 19598113)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
White
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
  • Wild-type female x Hemizygous male from the colony or reciprocal mating
  • Homozygous female x Homozygous male from the colony
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037490-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037490-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.