Strain Name:
STOCK Gt(ROSA)26Sortm1.1(birA)Mejr Srftm1.1Wtp/Mmjax
Stock Number:
037511-JAX
Citation ID:
RRID:MMRRC_037511-JAX
Other Names:
Srf-flbio
Major Collection:

Gene Information

Gt(ROSA)26Sortm1.1(birA)Mejr
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Dies Meijer
Synonyms: Rosa26BirA, bira
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: beta geo, ROSA26, Gtrosa26, Gtrgeo26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
NCBI: 14910
Srftm1.1Wtp
Name: serum response factor; targeted mutation 1.1, William T Pu
Synonyms: Srf-flbio, CVDC/B2B program mutation
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
Srf
Name: serum response factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
NCBI: 20807
VEGA: 17
Homologene: 31135
Genetic Alterations

For allele Srftm1Wtp: A targeting vector was designed to insert a FLAG/bio epitope tag and an frt-flanked neomycin resistance (neo) cassette at the endogenous stop codon and upstream of the 3' UTR.

For allele Gt(ROSA)26Sortm1(birA)Mejr: The allele was created using an HA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase), gene inserted into exon 3 of the rabbit beta-globin gene. The entire birA ORF, with the rabbit beta-globin splice acceptor and transcription termination sequences, were excised to generate a targeting vector that also contained a floxed PGK- puromycin selection cassette. This targeting vector was inserted into the first intron of the Gt(ROSA)26Sor gene.

This strain was developed by the Cardiovascular Development Consortium (CvDC) of the Bench to Bassinet Program.

Genotype Determination
ES Cell Line

Gt(ROSA)26Sortm1.1(birA)Mejr: IB10 (129P2/OlaHsd)

Srftm1.1Wtp: J1 (129S4/SvJae)

Phenotype
These biallelic mutant mice possess a FLAG/bio epitope tag downstream of the 3' UTR of Srf. Srf (serum response factor) is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors, which binds to the serum response element (SRE) in the promoter region of target genes.

These mice also contain the Gt(ROSA)26Sortm1.1(birA)Mejr allele, which expresses HA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase). Mice homozygous for both alleles are viable and fertile.
Mammalian Phenotype Terms
Allelic Composition: Srftm1.1Wtp/Srftm1.1Wtp (Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL * Swiss Webster )

  • normal phenotype
MeSH Terms
  • Animals
  • Biotinylation/methods
  • Carbon-Nitrogen Ligases/genetics
  • Carbon-Nitrogen Ligases/metabolism
  • Escherichia coli Proteins/genetics
  • Escherichia coli Proteins/metabolism
  • Female
  • GATA1 Transcription Factor/genetics
  • GATA1 Transcription Factor/metabolism
  • Genetic Vectors
  • Liver/cytology
  • Liver/embryology
  • Liver/metabolism
  • Mice
  • Mice, Transgenic
  • Octamer Transcription Factor-6/genetics
  • Octamer Transcription Factor-6/metabolism
  • Proteins/genetics
  • RNA, Untranslated
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Schwann Cells/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription, Genetic
  • Transfection
Strain Development
The Srf construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to outbred Swiss Webster mice. Offspring were bred with Tg(ACTFLPe)9205Dym transgenic mice to delete the neo cassette. The resulting flbio mice were maintained on a Swiss Webster background.

The construct for Gt(ROSA)26Sortm1.1(birA)Mejr was electroporated into 129P2/OlaHsd derived IB10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to FVB/N mice. The mice were intercrossed for 3 generations before being crossed to a Cre recombinase expressing strain (on an unknown genetic background) to remove the loxP-flanked PGK-puromycin selection cassette. A single loxP site remains. The Cre transgene was removed by breeding.
Suggested Control Mice
Wild-type littermates
  • Research Tool

Donor
William Pu, M.D., Children's Hospital Boston, Harvard Medical School.
Dies Meijer, Ph.D., The University of Edinburgh, Centre for Neuroregeneration and Centre for Clinical Brain Sciences.
Primary Reference
Driegen S, Ferreira R, van Zon A, Strouboulis J, Jaegle M, Grosveld F, Philipsen S, Meijer D. A generic tool for biotinylation of tagged proteins in transgenic mice. Transgenic Res. 2005 Aug;14(4):477-82. (Medline PMID: 16201414)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
MMRRC Breeding System
Intra-strain random mating
Breeding Scheme(s)

Homozygous female x Homozygous male from within the colony or reciprocal mating

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037511-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037511-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.