Strain Name:
B6;SJL-Akap6tm1.1Mskf/Mmjax
Stock Number:
037540-JAX
Citation ID:
RRID:MMRRC_037540-JAX
Other Names:
mAKAPfl/fl mouse,

Gene Information

Akap6tm1.1Mskf
Name: A kinase (PRKA) anchor protein 6; targeted mutation 1.1, Michael S Kapiloff
Synonyms: mAKAPfl, mAKAPfl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Targeted Mutation
Akap6
Name: A kinase (PRKA) anchor protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Targeted Mutation
NCBI: 238161
VEGA: 12
HGNC: HGNC:376
Homologene: 3157
Genetic Alterations
mAKAP Ex9fl/fl
Genotype Determination
ES Cell Line
Not applicable
Phenotype

Homozygous: no abnormal phenotype detected. 

Hetero/Hemizygous: no abnormal phenotype detected.

Cre-excised Phenotype: Conditional, cardiac myocyte-specific gene deletion of mAKAPβ expression in mice prevents the development of cardiac hypertrophy induced by pressure overload and catecholamine toxicity.

MeSH Terms
  • A Kinase Anchor Proteins/biosynthesis
  • A Kinase Anchor Proteins/genetics
  • Animals
  • Apoptosis
  • Cardiomegaly/genetics
  • Cardiomegaly/metabolism
  • Cardiomegaly/pathology
  • Disease Models, Animal
  • Gene Expression Regulation
  • Heart Failure/genetics
  • Heart Failure/metabolism
  • Heart Failure/physiopathology
  • Mice
  • Mice, Knockout
  • Myocardium/metabolism
  • Myocardium/pathology
  • Myocytes, Cardiac/metabolism
  • Myocytes, Cardiac/pathology
  • RNA/genetics
  • Signal Transduction
  • Ventricular Remodeling
Strain Development
mAKAPfl Allele: A targeting vector containing negative (tk) and positive (neo) selectable markers was designed to conditionally delete Exon 9 of the mAKAP (AKAP6) gene.Targeted C57BL/6 ES cell clones were confirmed by Southern blot using probes “a” and ‘”b”. The targeting vector introduced additional Xmn I and Nco I sites into the mAKAP gene.

After breeding of targeted mice, the neo cassette was deleted by mating to a FLP recombinase transgenic mouse (B6;SJL-Tg(ACTFLPe)9205Dym/J, Jackson Laboratory). Mouse genotyping was performed by PCR with primers 44 and 45 and genomic DNA obtained by tail biopsy.
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Cell Biology
  • Internal Organ
  • Research Tools
Donor
Michael Kapiloff, M.D., Ph.D., University of Miami, Miller School of Medicine.
Primary Reference

Kritzer MD, Li J, Passariello CL, Gayanilo M, Thakur H, Dayan J, Dodge-Kafka K, Kapiloff MS. The scaffold protein muscle A-kinase anchoring protein beta orchestrates cardiac myocyte hypertrophic signaling required for the development of heart failure. Circ Heart Fail. 2014 Jul;7(4):663-72. (Medline PMID: 24812305)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Heterozygous female x Heterozygous male littermate
  • Homozygous female x Homozygous male littermate
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Average litter size
5-9
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037540-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037540-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.