Strain Name:
FVB/N-Tg(Mylpf-cre)3Kraj/Mmjax
Stock Number:
037580-JAX
Citation ID:
RRID:MMRRC_037580-JAX
Other Names:
CRE3, MLC2v-CRE3, MLCcre

Strain Information

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Mylpf
Name: myosin light chain, phosphorylatable, fast skeletal muscle
Synonyms: 2410014J02Rik, MLC-2, MYL11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17907
Homologene: 22728
Tg(Mylpf-cre)3Kraj
Name: transgene insertion 3, Stan Krajewski
Synonyms: CRE3, MLC2v-CRE3, MLCcre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations
These CRE3 transgenic mice express Cre recombinase under the control of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter beginning at E11.5 in the brain. This strain may be useful for generating conditional deletions in neurons and their progenitors throughout the gray matter of the brain, cerebellum, spinal cord, as well as retina, dorsal, and sympathetic ganglia.
Genotype Determination
ES Cell Line
Not applicable
Phenotype
These CRE3 transgenic mice express Cre recombinase under the control of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter. Cre activity is observed beginning at E11.5 in the brain and only in cells that are committed to be neurons. Expression is found in the grey matter of the brain, cerebellum, spinal cord, retina, and dorsal and sympathetic ganglia. No expression is observed in the heart. CRE3 mice may be useful for generating conditional deletions in neurons and their progenitors.
MeSH Terms
  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2/genetics
  • Crosses, Genetic
  • Diabetes Complications/metabolism
  • Diabetes Complications/pathology
  • Diabetes Complications/physiopathology
  • Diabetes Mellitus/metabolism
  • Diabetes Mellitus/pathology
  • Diabetes Mellitus/physiopathology
  • Eating
  • Female
  • Hyperglycemia/metabolism
  • Hyperglycemia/physiopathology
  • Hyperinsulinism/metabolism
  • Hyperinsulinism/physiopathology
  • Insulin Resistance
  • Leptin/pharmacology
  • Leptin/physiology
  • Male
  • Mice
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Neurons/metabolism
  • Obesity/metabolism
  • Obesity/pathology
  • Obesity/physiopathology
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11/physiology
  • Signal Transduction
  • Gene Expression Profiling
  • Immunohistochemistry
  • Integrases/biosynthesis
  • Integrases/genetics
  • Mice, Knockout
  • Nervous System/chemistry
  • Neurons/physiology
  • beta-Galactosidase/genetics
  • Blood-Brain Barrier/drug effects
  • Blood-Brain Barrier/metabolism
  • Blood-Brain Barrier/pathology
  • Brain Injuries/chemically induced
  • Brain Injuries/enzymology
  • Brain Injuries/pathology
  • Brain Injuries/physiopathology
  • Caspase 8/genetics
  • Caspase 8/metabolism
  • Cell Death/drug effects
  • Cell Death/genetics
  • Cells, Cultured
  • Cerebral Cortex/drug effects
  • Cerebral Cortex/metabolism
  • Cerebral Cortex/pathology
  • Cerebral Cortex/physiopathology
  • Embryo, Mammalian
  • Gene Deletion
  • Gliosis/complications
  • Inflammation/complications
  • Kainic Acid/toxicity
  • Memory/drug effects
  • Memory/physiology
  • Neurons/drug effects
  • Neurons/enzymology
  • Neurons/pathology
  • Neurotoxins/toxicity
  • Seizures/chemically induced
  • Seizures/complications
  • Tumor Necrosis Factor-alpha/pharmacology
Strain Development
The transgenic construct contains Cre recombinase under the direction of a 250 bp fragment of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter. This transgene was microinjected into fertilized oocytes from a FVB/N female. Founder line 3 was established and maintained on an FVB/N background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Apoptosis
  • Diabetes
  • Endocrine Deficiency
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Sensorineural
Donor
Stan Krajewski, M.D., Ph.D., Sanford|Burnham Medical Research Institute.
Primary Reference
  • Banares S, Zeh K, Krajewska M, Kermer P, Baribault H, Reed JC, Krajewski S. Novel pan-neuronal Cre-transgenic line for conditional ablation of genes in the nervous system. Genesis. 2005 May;42(1):6-16. (Medline PMID: 15828007)
  • Krajewska M, Banares S, Zhang EE, Huang X, Scadeng M, Jhala US, Feng GS, Krajewski S. Development of diabesity in mice with neuronal deletion of Shp2 tyrosine phosphatase. Am J Pathol. 2008 May;172(5):1312-24. Epub 2008 Apr 10. (Medline PMID: 18403587)
  • Krajewska M1, You Z, Rong J, Kress C, Huang X, Yang J, Kyoda T, Leyva R, Banares S, Hu Y, Sze CH, Whalen MJ, Salmena L, Hakem R, Head BP, Reed JC, Krajewski S. Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity. PLoS One. 2011;6(9):e24341. Epub 2011 Sep 16. (Medline PMID: 21957448)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
White
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months >12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037580-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
037580-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.