Strain Name:
STOCK Zp2tm1Dean Tg(ZP2*)4Dean/Mmjax
Stock Number:
037592-JAX
Citation ID:
RRID:MMRRC_037592-JAX
Other Names:
Mo/hu Rescue

Gene Information

Zp2tm1Dean
Name: zona pellucida glycoprotein 2; targeted mutation 1, Jurrien Dean
Synonyms: Zp2tm1Nih, Zp2tm2Nih, Zp2tm
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Zp2
Name: zona pellucida glycoprotein 2
Synonyms: Zp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
NCBI: 22787
Homologene: 48194
ZP2
Name: zona pellucida glycoprotein 2 (sperm receptor)
Type: Gene
Species: Homo sapiens (human)
Chromosome: 16
Alteration at locus: Knockout
Tg(ZP2*)4Dean
Name: transgene insertion 4, Jurrien Dean
Synonyms: mo/huZP2
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations

Zp2tm1Dean: Part of exon 1 and exon 2 were replaced by a neomycin cassette. Absence of the protein was confirmed by immunohistochemistry on ovarian sections of mutant female mice

Tg(ZP2*)4Dean: The full-length sequence of the human ZP2 gene in BAC RP11-1023A8 was modified, putting in place the mouse exons encoding amino acids 18-156 (bp 1 in exon 2 to bp 150 in exon 5) for the corresponding exons of the human gene, encoding amino acids 22-161 (bp 1 in exon 2 to bp 152 in exon 5).

Genotype Determination
ES Cell Line

ES cells for each allele:

  • Zp2tm1Dean: R1 from (129X1/SvJ x 129S1/Sv)F1-Kitl+
  • Tg(ZP2*)4Dean: Not applicable
Mammalian Phenotype Terms
Allelic Composition: Zp2tm1Dean/Zp2tm1Dean (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CF-1 )

MeSH Terms
  • Animals
  • Egg Proteins/chemistry
  • Egg Proteins/genetics
  • Egg Proteins/metabolism
  • Female
  • Humans
  • Ligands
  • Male
  • Membrane Glycoproteins/chemistry
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/metabolism
  • Mice
  • Mice, Transgenic
  • Models, Biological
  • Protein Structure, Tertiary
  • Receptors, Cell Surface/chemistry
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism
  • Sperm-Ovum Interactions/genetics
  • Spermatozoa/physiology
  • Zona Pellucida Glycoproteins
Strain Development
Transgenic FVB animals were crossed into Zp2-null background to obtain rescue mice without backcrossing to congenicity. The line's genetic background is a mix of FVB/N, 129X1/SvJ, and 129S1/Sv (i.e., STOCK).
Suggested Control Mice
Wild-type littermates
  • Cell Biology
  • Developmental Biology
  • Reproduction
Donor
Jurrien Dean, M.D., National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health.
Primary Reference

Avella MA, Baibakov B, and Dean J. A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans. J Cell Biol. 2014 Jun 23;205(6):801-9. Epub 2014 Jun 16 (Medline PMID: 24934154)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Varied
Eye
Varied
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Homozygous/Homozygous female x Homozygous/Heterozygous male
  • Heterozygous/Hemizygous female x Heterozygous/Hemizygous male
  • Wild-type female x Heterozygous/Hemizygous male
Generation
N2F3
Overall Breeding Performance
Poor
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Reduced Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 5 months 5 months
Average litter size
4-6
Recommended wean age
3-4 weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037592-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037592-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.