Strain Detail Sheet

Strain Name:
C57BL/6N-Tg(Tek-Scarb1)14Arem/Mmjax
Stock Number:
037626-JAX
Citation ID:
RRID:MMRRC_037626-JAX
Other Names:
mTie2-Sr-b1, Tie2-Sr-b1

Strain Information

Gene Details

Scarb1
Name: scavenger receptor class B, member 1
Synonyms: D5Ertd460e, Srb1, Cd36l1, Cla-1, SRBI, SR-BI, Hlb398, Hdlq1, Chohd1, Chohd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20778
HGNC: HGNC:1664
Homologene: 21132
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Tek
Name: TEK receptor tyrosine kinase
Synonyms: Hyk, Tie2, Cd202b, tie-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21687
Homologene: 397
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tg(Tek-Scarb1)14Arem
Name: transgene insertion 14, Alan Remaley
Synonyms: Tie2-SR-B1
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations
Tie2-SR-B1 transgenic mice express the scavenger receptor class B, member 1 (Scarb1) under the control of the endothelial-specific receptor tyrosine kinase (Tek) promoter. This strain may be useful for studying HDL metabolism and cholesterol trafficking in endothelial cells, and protection against atherosclerosis.

Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype
Tie2-SR-B1 transgenic mice express the scavenger receptor class B, member 1 (Scarb1) under the control of the endothelial-specific receptor tyrosine kinase (Tek) promoter. SCARB1 is involved in the transfer of cholesterol between cells and high density lipoprotein cholesterol (HDLC). Overexpression of SCARB1 in endothelial cells results in decreased forward-facing surface of aortic lesions in mice with diet-induced atherosclerosis on the C57BL/6 background. Transfer of HDL through endothelial cells is approximately 4 fold faster from the apical to basolateral compartment than in the reverse direction. This strain may be useful for studying HDL metabolism and cholesterol trafficking in endothelial cells, and protection against atherosclerosis.
Strain Development
The Tie2-SR-B1 transgene contains mouse Scarb1 cDNA under the control of the endothelial-specific receptor tyrosine kinase (Tek) promoter. The construct was microinjected into C57BL/6NJ fertilized oocytes. The founder mouse 14 was mated to C57BL/6NTac. Upon arrival, mice were bred to C57BL/6NJ for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates or C57BL/6N mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Metabolism

Strain Origin

Donor
Alan Remaley, D.V.M., National Heart, Lung, and Blood Institute (NHLBI).
Primary Reference

Vaisman BL, Ghias M, Demosky SJ, Stonik JA, Amar MJ, Sampson M, Remaley AT. Role of endothelial expression of ABCA1 and SR-BI genes in HDL metabolism, cholesterol trafficking and protection against atherosclerosis. Abstract from AHA Scientific Sessions 2010, Chicago, IL.

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Average litter size
4-6
Recommended wean age
4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.