Strain Name:
B6.Cg-Apoetm1Unc Tg(MSR1-TXN*)23Arem/Mmjax
Stock Number:
037627-JAX
Citation ID:
RRID:MMRRC_037627-JAX
Other Names:
TRX-80 x apoE-Ko

Gene Information

Apoetm1Unc
Name: apolipoprotein E; targeted mutation 1, University of North Carolina
Synonyms: apoE-, apoE0, APOE KO, AopE(-), mEKO, mE-, EKO, ApoE-KO, Apoetm1Un, epsilon-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Apoe
Name: apolipoprotein E
Synonyms: Apoe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
NCBI: 11816
HGNC: HGNC:613
Homologene: 30951
TXN
Name: thioredoxin
Type: Gene
Species: Homo sapiens (human)
Chromosome: 9
Alteration at locus: Knockout
MSR1
Name: macrophage scavenger receptor 1
Type: Gene
Species: Homo sapiens (human)
Chromosome: 8
Alteration at locus: Knockout
Tg(MSR1-TXN*)23Arem
Name: transgene insertion 23, Alan Remaley
Synonyms: TRX80
Type: Transgene
Species: Homo sapiens (human)
Chromosome: unknown
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations
Human TXN (TRX80) transgene under control of the human MSR1 (SR-A) promoter was injected into fertilized eggs from C57BL/6N females. The transgene was then transferred onto the Apoe-knockout background.
Genotype Determination
ES Cell Line
E14TG2a (129P2/OlaHsd) for the KO allele.
Phenotype

Homozygous: The strain is viable

Hemizygous: The strain is viable.

Mammalian Phenotype Terms
Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: B6.129P2-Apoetm1Unc )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: B6.129P2-Apoetm1Unc/J )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: B6.Cg-Apoetm1Unc Faslpr )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: either: B6.129P2-Apoetm1Unc/J or (involves: 129P2/OlaHsd * C57BL/6J * ICR) )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N )

Allelic Composition: Apoetm1Unc/Apoetm1Unc (Genetic Background: Not Specified )

  • adipose tissue
  • hematopoietic system
  • homeostasis/metabolism
    • decreased circulating glucose level [MP:0005560]
    • increased circulating cholesterol level [MP:0005178]
    • increased circulating HDL cholesterol level [MP:0001556]
    • increased circulating LDL cholesterol level [MP:0000182]
    • increased circulating triglyceride level [MP:0001552]
    • increased circulating alkaline phosphatase level [MP:0002968]
    • improved glucose tolerance [MP:0005292]
  • immune system
    • increased susceptibility to bacterial infection [MP:0002412]
  • growth/size/body
Allelic Composition: Apoetm1Unc/Apoe+ (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Allelic Composition: Apoetm1Unc/Apoe+ (Genetic Background: Not Specified )

  • hematopoietic system
  • immune system
    • increased susceptibility to bacterial infection [MP:0002412]
Allelic Composition: Apoeshl/Apoetm1Unc (Genetic Background: involves: 129P2/OlaHsd * KOR )

MeSH Terms
  • ADAM Proteins/metabolism
  • ADAM10 Protein
  • ADAM17 Protein
  • Aged
  • Aged, 80 and over
  • Alzheimer Disease/pathology
  • Amyloid Precursor Protein Secretases/metabolism
  • Amyloid beta-Peptides/metabolism
  • Brain/pathology
  • Cerebrospinal Fluid/chemistry
  • Female
  • Humans
  • Male
  • Membrane Proteins/metabolism
  • Middle Aged
  • Peptide Fragments/metabolism
  • Thioredoxins/metabolism
Strain Development

The transgene was inserted into C57BL/6NJ mice. The transgene was then transferred onto the apoE-Ko background by crossing with JAX Strain #0002052 B6.129P2-Apoetm1Unc/J, on a congenic C57BL/6 background.

Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Models for Human Disease
  • Neurobiology
Donor
Alan Remaley, D.V.M., National Heart, Lung, and Blood Institute (NHLBI).
Primary Reference

Gil-Bea F, Akterin S, Persson T, Mateos L, Sandebring A, Avila-Cari o J, Gutierrez-Rodriguez A, Sundstr m E, Holmgren A, Winblad B, Cedazo-Minguez A. Thioredoxin-80 is a product of alpha-secretase cleavage that inhibits amyloid-beta aggregation and is decreased in Alzheimer's disease brain. EMBO Mol Med. 2012 Oct;4(10):1097-111. Epub 2012 Aug 30. (Medline PMID:22933306)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 5 months 5 months
Average litter size
4-6
Recommended wean age
3-4 weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037627-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
037627-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.