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Strain Name:
NOD.Cg-Ifngtm1Ts H2h4 CD40lgtm1Imx/HbmMmmh
Stock Number:
037631-MU
Citation ID:
RRID:MMRRC_037631-MU
Other Names:
CD154-/-IFNg-/-NOD.H-2h4
Major Collection:

Strain Information

Ifngtm1Ts
Name: interferon gamma; targeted mutation 1, Timothy Stewart
Synonyms: IFN-gamma KO, Ifngtm1Ts, gko, ifntm1Ts, ifgtm1, Ifngtm1Ts, IFN-g KO, Ifntm/Ts, GKO, IFN-gammatm1Ts, ifng-, IFNgamma KO, IFN-gammaKO, IFN-gamma-, Ifn-gtm1Ts, IFNgamma-, IFN-gamma KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Cd40lgtm1Imx
Name: CD40 ligand; targeted mutation 1, Immunex
Synonyms: CD40L-, CD40LKO, Tnfsf5tm1Imx, CD154-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
Ifng
Name: interferon gamma
Synonyms: IFN-gamma, Ifg
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knockout
NCBI: 15978
VEGA: 10
HGNC: HGNC:5438
Homologene: 55526
Cd40lg
Name: CD40 ligand
Synonyms: CD154, gp39, Ly-62, Cd40L, T-BAM, HIGM1, IMD3, Tnfsf5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
NCBI: 21947
Homologene: 56
H2h4
Name: histocompatibility-2, MHC; h4 variant
Synonyms: H-2h4
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Polymorphic allele
H2
Name: histocompatibility-2, MHC
Synonyms: H-2, MHC-II
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Polymorphic allele
Genetic Alterations
  • CD40lg allele: Exons 3 and 4 were replaced with a neomycin cassette. Homozygous mutant animals fail to express a functional protein on the cell surface.
  • Ifng allele: Insertion of a neomycin gene into exon 2, which introduced a termination codon after the first 30 amino acids of the mature protein.
Genotype Determination
ES Cell Line
  • CD40lg allele: D3 derived from 129S2/SvPas
  • Ifng allele: AB1 derived from 129S7/SvEvBrd-Hprt+
Phenotype

Homozygous: resistant to the autoimmune thyroid disease TEC H/P

Heterozygous: Undetermined

Mammalian Phenotype Terms
Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: B6.129S7-Ifngtm1Ts )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: B6.129S7-Ifngtm1Ts/J )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: C.129S7(B6)-Ifngtm1Ts/J )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: C.129S7-Ifngtm1Ts )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * A/WySn * C57BL/10SnSg * Swiss )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * BALB/c )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * DBA/1 * MRL/Mp )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: involves: 129S7/SvEvBrd * NOD )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: NOD.129S7(B6)-Ifngtm1Ts Prkdcscid )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: NOD.129S7(B6)-Ifngtm1Ts/DvsJ )

Allelic Composition: Ifngtm1Ts/Ifngtm1Ts (Genetic Background: NOD.129S7-Ifngtm1Ts )

Allelic Composition: H2h4/H2h4 (Genetic Background: NOD.Cg-H2h4 )

Allelic Composition: H2h4/H2g7 (Genetic Background: NOD.Cg-H2h4 )

MeSH Terms
  • Animals
  • Autoimmunity
  • CD4-Positive T-Lymphocytes/immunology
  • CD4-Positive T-Lymphocytes/metabolism
  • CD40 Antigens/genetics
  • CD40 Antigens/metabolism
  • Diabetes Mellitus, Type 1/immunology
  • Forkhead Transcription Factors/immunology
  • Forkhead Transcription Factors/metabolism
  • Interferon-gamma/biosynthesis
  • Interleukin-17/biosynthesis
  • Mice
  • Mice, Inbred NOD
  • Mice, Knockout
  • Prediabetic State/immunology
  • Receptors, Antigen, T-Cell/immunology
Strain Development
The strain was derived by crossing B6.CD154-/- mice (likely JAX Strain #002770) to NOD for 10 generations. Resulting males were then crossed with NOD.Cg-Ifngtm1Ts H2h4/HbmMmmh females (MMRRC:37140). The F1 offspring were further crossed and selected for expression of the H2h4 MHC by flow cytometry of peripheral blood and for expression of the CD154 (CD40lg) and Ifng knockout genes by PCR analysis of tail DNA. Once established, further mice were generated by breeding homozygous double knockouts
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Immunology and Inflammation

Donor
Helen Braley-Mullen, Ph.D., University of Missouri.
Timothy A. Stewart, Ph.D., Genentech.
Charles R. Maliszewski, Ph.D., Immunex.
Primary Reference

Vaitaitas GM, Carter JR, Waid DM, Olmstead MH, Wagner DH. An Alternative Role for Foxp3 As an Effector T Cell Regulator Controlled through CD40. J Immunol. 2013 Jul 15;191(2):717-25. (Medline PMID: 23776180)

Additional References

Podolin PL, Pressey A, DeLarato NH, Fischer PA, Peterson LB, Wicker LS. I-E+nonobese diabetic mice develop insulitis and diabetes. J Exp Med. 1993 Sep1;178(3):793-803. (Medline PMID: 8350054)

Dalton DK, Pitts-Meek S, Keshav S, Figari IS, Bradley A, Stewart TA. Multiple defects of immune cell function in mice with disrupted interferon-gamma genes.Science. 1993 Mar 19;259(5102):1739-42. (Medline PMID: 8456300)

Renshaw BR, Fanslow WC 3rd, Armitage RJ, Campbell KA, Liggitt D, Wright B,Davison BL, Maliszewski CR. Humoral immune responses in CD40 ligand-deficientmice. J Exp Med. 1994 Nov 1;180(5):1889-900. (Medline PMID: 7964465)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
White (albino)
Eye
Pink
MMRRC Breeding System
Backcross then sib-mating
Generation
N10 (NOD)
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3 weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037631-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037631-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037631-MU-RESUS Litter recovered from cryo-archive $2,028.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.