Strain Detail Sheet

Strain Name    :

B6.129S4-Sptnb4Gt(ROSABetageo)62Sor/Mmucd

Stock Number :

000038-UCD

Other Names   :

ROSA 62, B6.129S4-Spnb4Gt(ROSABetageo)62Sor/Mmcd, B6.129S4-Spnb4Gt(ROSAβgeo)62Sor/Ucd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Sptbn4Gt(ROSABetageo)62Sor
Name: gene trap 62, Philippe Soriano
Chromosome: 7
Alteration at locus: Gene Trap
Gene Symbol: Sptbn4
Name: spectrin beta, non-erythrocytic 4
Chromosome: 7
Alteration at locus: Gene Trap

Genetic Alterations:
Gene trap insertion of ROSA beta-geo.

Genotype Determination:

ES Cell Line: AK7.1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Tremors and contraction of hindlimbs (recessive phenotype).


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Spnb4Gt(ROSABetageo)62Sor/Spnb4Gt(ROSABetageo)62Sor
        B6.129S4-Spnb4<Gt(ROSABetageo)62Sor>/Mmcd
  • behavior/neurological phenotype
    • ataxia (MGI Ref ID J:77228)
      • mice cannot walk at 6-10 months of age
    • limb grasping (MGI Ref ID J:77228)
    • tremors (MGI Ref ID J:77228)
      • first observable at weaning, continue and worsen with age
  • muscle phenotype
    • abnormal muscle contractility (MGI Ref ID J:77228)
      • mice 6-10 months of age exhibit continuous contraction of hind limb skeletal muscle


The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Spnb4Gt(ROSABetageo)62Sor/Spnb4Gt(ROSABetageo)62Sor
        129S4/SvJaeSor-Spnb4<Gt(ROSABetageo)62Sor>
  • behavior/neurological phenotype
    • ataxia (MGI Ref ID J:77228)
      • mice cannot walk at 6-10 months of age
    • limb grasping (MGI Ref ID J:77228)
    • tremors (MGI Ref ID J:77228)
      • first observable at weaning, continue and worsen with age
  • muscle phenotype
    • abnormal muscle contractility (MGI Ref ID J:77228)
      • mice 6-10 months of age exhibit continuous contraction of hind limb skeletal muscle
Spnb4Gt(ROSABetageo)62Sor/Spnb4Gt(ROSABetageo)62Sor
        involves: 129S4/SvJaeSor * C57BL/6J
  • behavior/neurological phenotype
    • abnormal mating frequency (MGI Ref ID J:77228)
      • males reproduce very inefficiently when mated with wild-type females
      • however, no histological abnormalities are detected and when mating occurs normal litter sizes are produced
    • ataxia (MGI Ref ID J:77228)
      • mice cannot walk at 6-10 months of age
    • limb grasping (MGI Ref ID J:77228)
    • tremors (MGI Ref ID J:77228)
      • first observable at weaning, continue and worsen with age
  • muscle phenotype
    • abnormal muscle contractility (MGI Ref IDs J:120916, J:77228)
      • mice 6-10 months of age exhibit continuous contraction of hind limb skeletal muscle (MGI Ref ID J:77228)
      • at older than 8 months mice often exhibit continuous contraction of hindleg muscle (MGI Ref ID J:120916)
  • nervous system phenotype
    • abnormal axon morphology (MGI Ref ID J:120916)
      • ankyrin G+ regions of the neurons, but not Purkinje cells, in the cerebral cortex are longer
    • abnormal myelination (MGI Ref ID J:120916)
      • plasma membranes at the Node of Ranvier in the optic nerve are wavy and swollen

Strain of Origin: 129S4/SvJae

Strain genetic background: C57BL/6J

Strain Development: Gene trap insertion of ROSA beta-geo in AK7 ES cell line from 129S4/SvJae. Backcrossed to C57BL/6J for 12 generations. The vector used in this mutant contains a betageo that has an aspartic acid at residue 182.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Neurobiology
  • Sodium channel fields

Strain Origin

Donor: Philippe Soriano, Fred Hutchinson Cancer Research Center

Primary Reference:

Komada, M. and Soriano, P. BetaIV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier. J Cell Biol. 2002 Jan 21; 156(2):337-48. (Medline PMID: 11807096 )

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Sib-mating

Breeding Scheme(s):

  • Heterozygous Female x Heterozygous Male
  • Backcross to C57BL/6J

Generation: N12

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 40 weeks 40 weeks

Average litter size: 6

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000038-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000038-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
000038-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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