Strain Name:
C57BL/6J-MtgxP0016Btlr/Mmmh
Stock Number:
038269-MU
Citation ID:
RRID:MMRRC_038269-MU
Other Names:
P0016 (G1), C57BL/6J-MtgxP0016Btlr
Major Collection:

Gene Information

Slamf6
Name: SLAM family member 6
Synonyms: SF2000, NTB-A, KAL1b, KAL1, Ly108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 30925
Homologene: 49945
Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Traip
Name: TRAF-interacting protein
Synonyms: Trip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22036
Homologene: 31343
Mbd1
Name: methyl-CpG binding domain protein 1
Synonyms: PCM1, Cxxc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17190
VEGA: 18
HGNC: HGNC:6916
Homologene: 8414
Rnaseh2a
Name: ribonuclease H2, large subunit
Synonyms: 2400006P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 69724
Homologene: 4664
Zfp799
Name: zinc finger protein 799
Synonyms: 6030490I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240064
Homologene: 107106
Slain1
Name: SLAIN motif family, member 1
Synonyms: 9630044O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105439
Homologene: 16943
Ctnnd2
Name: catenin (cadherin associated protein), delta 2
Synonyms: Nprap, Catnd2, neurojugin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18163
VEGA: 15
HGNC: HGNC:2516
Homologene: 55574
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Mroh7
Name: maestro heat-like repeat family member 7
Synonyms: LOC381538, Gm1027, Heatr8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381538
Homologene: 19633
Klb
Name: klotho beta
Synonyms: betaKlotho
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83379
Homologene: 12820
1700123K08Rik
Name: RIKEN cDNA 1700123K08 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76658
Homologene: 86127
4930432E11Rik
Name: RIKEN cDNA 4930432E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243900
Myo16
Name: myosin XVI
Synonyms: C230040D10Rik, Nyap3, BM140241
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244281
Homologene: 34710
Zfp750
Name: zinc finger protein 750
Synonyms: A030007D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319530
Homologene: 36423
Kif27
Name: kinesin family member 27
Synonyms: 4930517I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75050
VEGA: 13
Homologene: 69235
Dennd6b
Name: DENN/MADD domain containing 6B
Synonyms: 1700027J05Rik, Fam116b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69440
VEGA: 15
Homologene: 57065
Arap3
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Synonyms: DRAG1, E030006K04Rik, Centd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 106952
VEGA: 18
Homologene: 11199
Rbm22
Name: RNA binding motif protein 22
Synonyms: 8430430L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 66810
Homologene: 69245
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 171,936,501 bp
  • T to C, chromosome 2 at 76,811,183 bp
  • T to A, chromosome 4 at 106,707,857 bp
  • G to A, chromosome 5 at 65,379,923 bp
  • A to T, chromosome 5 at 138,562,938 bp
  • T to C, chromosome 7 at 29,563,112 bp
  • C to T, chromosome 8 at 10,400,596 bp
  • C to G, chromosome 8 at 84,959,800 bp
  • A to G, chromosome 9 at 107,968,656 bp
  • T to A, chromosome 11 at 121,513,978 bp
  • G to A, chromosome 13 at 58,303,452 bp
  • A to G, chromosome 14 at 103,685,674 bp
  • G to A, chromosome 15 at 30,966,938 bp
  • C to T, chromosome 15 at 38,000,578 bp
  • T to C, chromosome 15 at 89,186,977 bp
  • T to C, chromosome 17 at 32,819,357 bp
  • T to A, chromosome 18 at 37,984,348 bp
  • T to A, chromosome 18 at 60,570,770 bp
  • C to T, chromosome 18 at 74,274,538 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for P0016 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038269-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.