Strain Name:
C57BL/6J-MtgxP0018Btlr/Mmmh
Stock Number:
038271-MU
Citation ID:
RRID:MMRRC_038271-MU
Other Names:
P0018 (G1), C57BL/6J-MtgxP0018Btlr
Major Collection:

Gene Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 24069
Homologene: 9262
Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Katna1
Name: katanin p60 (ATPase-containing) subunit A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 23924
HGNC: HGNC:6216
Homologene: 56014
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268902
Homologene: 43188
Tmub1
Name: transmembrane and ubiquitin-like domain containing 1
Synonyms: Hops, 2010004O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 64295
Homologene: 11187
Cxxc1
Name: CXXC finger 1 (PHD domain)
Synonyms: 5830420C16Rik, 2410002I16Rik, PHF18, Cgbp, Cfp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 74322
VEGA: 18
Homologene: 32221
Brip1
Name: BRCA1 interacting protein C-terminal helicase 1
Synonyms: BACH1, 8030460J03Rik, 3110009N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237911
Homologene: 32766
Nlgn1
Name: neuroligin 1
Synonyms: 6330415N05Rik, NL1, Nlg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 192167
Homologene: 56690
AAdacl4fm3
Name: AADACL4 family member 3
Synonyms: Gm13178
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 546849
Homologene: 82586
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26875
Homologene: 69111
Nell1
Name: NEL-like 1
Synonyms: B230343H07Rik, l7R6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 338352
HGNC: HGNC:7750
Homologene: 4486
Hgsnat
Name: heparan-alpha-glucosaminide N-acetyltransferase
Synonyms: 9430010M12Rik, Tmem76, D8Ertd354e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52120
Homologene: 15586
Galnt2
Name: polypeptide N-acetylgalactosaminyltransferase 2
Synonyms: ppGaNTase-T2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108148
HGNC: HGNC:4124
Homologene: 3297
Ftsj3
Name: FtsJ RNA methyltransferase homolog 3 (E. coli)
Synonyms: D11Ertd400e, C79843, Epcs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56095
Homologene: 5451
Cspg4b
Name: chondroitin sulfate proteoglycan 4B
Synonyms: BC067074
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408066
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 68,071,676 bp
  • G to T, chromosome 3 at 25,436,577 bp
  • A to T, chromosome 4 at 144,703,197 bp
  • A to T, chromosome 5 at 14,677,721 bp
  • C to A, chromosome 5 at 24,446,757 bp
  • A to G, chromosome 7 at 50,120,691 bp
  • C to T, chromosome 8 at 25,968,354 bp
  • T to A, chromosome 8 at 124,336,611 bp
  • A to T, chromosome 10 at 7,741,459 bp
  • C to T, chromosome 11 at 86,108,868 bp
  • C to A, chromosome 11 at 106,254,808 bp
  • A to G, chromosome 13 at 113,367,506 bp
  • T to C, chromosome 16 at 74,046,806 bp
  • C to T, chromosome 18 at 74,220,921 bp
  • A to G, chromosome 19 at 46,475,494 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for P0018 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038271-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.