Strain Name:
C57BL/6J-MtgxP0028Btlr/Mmmh
Stock Number:
038281-MU
Citation ID:
RRID:MMRRC_038281-MU
Other Names:
P0028 (G1), C57BL/6J-MtgxP0028Btlr
Major Collection:

Strain Information

Dock1
Name: dedicator of cytokinesis 1
Synonyms: Dock180, 9130006G06Rik, D630004B07Rik, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Arhgef10
Name: Rho guanine nucleotide exchange factor 10
Synonyms: 6430549H08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234094
Homologene: 22827
Setd2
Name: SET domain containing 2
Synonyms: KMT3A, 4921524K10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235626
Homologene: 56493
Dusp12
Name: dual specificity phosphatase 12
Synonyms: mVH1, 1190004O14Rik, VH1, T-DSP4, LMW-DSP4, ESTM36
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 80915
HGNC: HGNC:3067
Homologene: 5238
Mical3
Name: microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms: MICAL-3, C130040D16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 194401
Homologene: 85288
Cc2d2a
Name: coiled-coil and C2 domain containing 2A
Synonyms: b2b1035Clo, 5730509K17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231214
Homologene: 18159
Fam171b
Name: family with sequence similarity 171, member B
Synonyms: D430039N05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241520
Homologene: 18462
Srp68
Name: signal recognition particle 68
Synonyms: 2610024I03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217337
Homologene: 7056
Arhgap17
Name: Rho GTPase activating protein 17
Synonyms: WBP15, Nadrin, 5730403H17Rik, Rich1, Nadrin2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70497
Homologene: 9984
Raf1
Name: v-raf-leukemia viral oncogene 1
Synonyms: c-Raf, Craf1, Raf-1, v-Raf, sarcoma 3611 oncogene, 6430402F14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 110157
HGNC: HGNC:9829
Homologene: 48145
Rb1
Name: RB transcriptional corepressor 1
Synonyms: pRb, Rb, retinoblastoma 1, Rb-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19645
HGNC: HGNC:9884
Homologene: 272
Ddx24
Name: DEAD box helicase 24
Synonyms: 2510027P10Rik, 1700055J08Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 27225
VEGA: 12
Homologene: 10702
Nbeal1
Name: neurobeachin like 1
Synonyms: A530083I02Rik, A530050O19Rik, 2310076G13Rik, ALS2CR17
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269198
Homologene: 16453
Prickle1
Name: prickle planar cell polarity protein 1
Synonyms: 1110058P22Rik, Pk1, mpk1, b2b019Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 106042
Homologene: 17686
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms: alpha myosin, cardiomyopathy, hypertrophic 1, alpha-MHC, Myhca, alphaMHC, alpha cardiac MHC, Myhc-a, A830009F23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 17888
HGNC: HGNC:7576
Homologene: 124414
Rcsd1
Name: RCSD domain containing 1
Synonyms: A430105K13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226594
Homologene: 33475
Zfp831
Name: zinc finger protein 831
Synonyms: OTTMUSG00000017459, ENSMUSG00000050600
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 100043757
Homologene: 19013
Unc13b
Name: unc-13 homolog B
Synonyms: Unc13h2, Munc13-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22249
Homologene: 31376
Celsr1
Name: cadherin, EGF LAG seven-pass G-type receptor 1
Synonyms: Crsh, Scy, crash, Adgrc1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12614
VEGA: 15
HGNC: HGNC:1850
Homologene: 7665
Rtp1
Name: receptor transporter protein 1
Synonyms: LOC239766, LOC385871
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239766
VEGA: 16
Homologene: 17806
Cutc
Name: cutC copper transporter
Synonyms: 2310039I18Rik, CGI-32
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66388
Homologene: 9318
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,291,937 bp
  • G to A, chromosome 1 at 165,655,997 bp
  • A to T, chromosome 1 at 170,879,817 bp
  • T to A, chromosome 2 at 83,853,439 bp
  • A to G, chromosome 2 at 174,645,346 bp
  • T to A, chromosome 4 at 43,256,225 bp
  • A to G, chromosome 5 at 43,684,199 bp
  • G to A, chromosome 6 at 115,631,205 bp
  • A to T, chromosome 6 at 121,024,689 bp
  • G to A, chromosome 7 at 123,286,677 bp
  • T to G, chromosome 7 at 134,999,324 bp
  • C to T, chromosome 8 at 14,928,925 bp
  • A to G, chromosome 9 at 110,573,954 bp
  • C to A, chromosome 11 at 116,260,920 bp
  • T to C, chromosome 12 at 103,408,375 bp
  • C to T, chromosome 14 at 54,963,637 bp
  • C to A, chromosome 14 at 73,264,628 bp
  • G to A, chromosome 15 at 85,922,235 bp
  • A to G, chromosome 15 at 93,500,902 bp
  • G to C, chromosome 16 at 23,429,366 bp
  • A to G, chromosome 19 at 43,764,969 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for P0028 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038281-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.