Strain Name:
B6.129X1-Ly6atm1Grau/Mmnc
Stock Number:
000383-UNC
Citation ID:
RRID:MMRRC_000383-UNC
Other Names:
Sca-1

Strain Information

Ly6atm1Grau
Name: lymphocyte antigen 6 family member A; targeted mutation 1, Timothy A Graubert
Synonyms: Sca-1EGFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Targeted Mutation
Ly6a
Name: lymphocyte antigen 6 family member A
Synonyms: Sca1, Ly-6A.2, Ly-6E.1, Sca-1, Ly-6A/E, TAP
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Targeted Mutation
NCBI: 110454
Homologene: 113718
Genetic Alterations
EGFP allele inserted into exon 2 of Sca-1 locus, replacing the Sca-1 initiation codon. PGK-neo deleted in ES cells via LoxP-Cre recombination.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    RW-4 derived from 129X1/SvJ
    Phenotype
    Homozygous: These animals are Sca-1 null. Only subtle effects noted in B cell development.

    Heterozygous: The heterozygtes express GFP under the control of the Sca-1 promoter. GFP can be used as a surrogate for Sca-1 in immunofluorescence and flow cytometric applications. Primitive hematopoietic stem cells are GFP+. Mammary gland progenitors are also GFP+.
    Mammalian Phenotype Terms
    Allelic Composition: Ly6atm1Grau/Ly6atm1Grau (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

    Allelic Composition: Ly6atm1Grau/Ly6a+ (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

    MeSH Terms
    • Animals
    • Antigens, Ly/genetics
    • Bone Marrow Cells/cytology
    • Bone Marrow Cells/metabolism
    • Cytological Techniques/methods
    • Genes, Reporter
    • Green Fluorescent Proteins
    • Hematopoietic Stem Cells/cytology
    • Hematopoietic Stem Cells/metabolism
    • Luminescent Proteins/genetics
    • Membrane Proteins/genetics
    • Mice
    • Mice, Transgenic/genetics
    • Mice, Transgenic/growth & development
    • Spleen/cytology
    • Spleen/metabolism
    • Antigens, Ly/metabolism
    • Cell Differentiation
    • Cell Division
    • Epithelial Cells/cytology
    • Epithelial Cells/immunology
    • Epithelial Cells/metabolism
    • Female
    • Fluorescent Antibody Technique
    • Mammary Glands, Animal/cytology
    • Mammary Glands, Animal/immunology
    • Mammary Glands, Animal/metabolism
    • Membrane Proteins/metabolism
    • Mice, Inbred C57BL
    • Stem Cells/cytology
    • Stem Cells/immunology
    • Stem Cells/metabolism
    Suggested Control Mice
    • Wildtype littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Hematology
    • Models for Human Disease
    • Research Tools
    Donor
    Timothy Graubert, M.D., Washington University School of Medicine
    Primary Reference
    • Hanson P, Mathews V, Marrus SH, Graubert TA. Enhanced green fluorescent protein targeted to the Sca-1 (Ly-6A) locus in transgenic mice results in efficient marking of hematopoietic stem cells in vivo. Exp Hematol. 2003 Feb;31(2):159-67. (Medline PMID: 12591281)
    • Welm BE, Tepera SB, Venezia T, Graubert TA, Rosen JM, Goodell MA. Sca-1(pos) cells in the mouse mammary gland represent an enriched progenitor cell population. Dev Biol. 2002 May 1;245(1):42-56. (Medline PMID: 11969254)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    000383-UNC-SPERM Cryo-preserved spermatozoa $564.00 / $937.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    000383-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.