Strain Detail Sheet

Strain Name    :

B6.129X1-Ly6atm1Grau/Mmnc

Stock Number :

000383-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Ly6atm1Grau
Name: targeted mutation 1, Timothy A Graubert
Alteration at locus: Targeted Mutation
Gene Symbol: Ly6a
Name: lymphocyte antigen 6 complex, locus A
Chromosome: 15
Alteration at locus: Targeted Mutation

Genetic Alterations:
EGFP allele inserted into exon 2 of Sca-1 locus, replacing the Sca-1 initiation codon. PGK-neo deleted in ES cells via LoxP-Cre recombination.

Genotype Determination:

ES Cell Line: RW-4 derived from C57BL/6J x 129/SvJ

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: These animals are Sca-1 null. Only subtle effects noted in B cell development.

Heterozygous phenotype: The heterozygtes express GFP under the control of the Sca-1 promoter. GFP can be used as a surrogate for Sca-1 in immunofluorescence and flow cytometric applications. Primitive hematopoietic stem cells are GFP+. Mammary gland progenitors are also GFP+.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Ly6atm1Grau/Ly6a+
        involves: 129X1/SvJ * C57BL/6
  • normal phenotype
    • no abnormal phenotype detected (MGI Ref ID J:82052)
Ly6atm1Grau/Ly6atm1Grau
        involves: 129X1/SvJ * C57BL/6
  • normal phenotype
    • no abnormal phenotype detected (MGI Ref ID J:82052)

Strain of Origin: C57BL/6J x 129/SvJ

Strain genetic background: C57BL/6J

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Hematology
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: Timothy Graubert, M.D., Washington University School of Medicine

Primary Reference:

  • Hanson P, Mathews V, Marrus SH, Graubert TA. Enhanced green fluorescent protein targeted to the Sca-1 (Ly-6A) locus in transgenic mice results in efficient marking of hematopoietic stem cells in vivo. Exp Hematol. 2003 Feb;31(2):159-67. PMID: 12591281 (Medline PMID: 12591281)
  • Welm BE, Tepera SB, Venezia T, Graubert TA, Rosen JM, Goodell MA. Sca-1(pos) cells in the mouse mammary gland represent an enriched progenitor cell population. Dev Biol. 2002 May 1;245(1):42-56. PMID: 11969254 (Medline PMID: 11969254 )

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Appearance

Coat color:

Other:

Breeding

MMRRC Breeding System:

Breeding Scheme(s):

Generation:

Overall Breeding Performance:

Breeding Comments: Back-cross to C57BL/6

Reproductive Statistics

Viability and Fertility: Unknown

Average litter size:

Recommended wean age:

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000383-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/24/2014