Strain Name:
C57BL/6J-MtgxR0048Btlr/Mmmh
Stock Number:
038342-MU
Citation ID:
RRID:MMRRC_038342-MU
Other Names:
R0048 (G1), C57BL/6J-MtgxR0048Btlr
Major Collection:

Gene Information

Tgfb1
Name: transforming growth factor, beta 1
Synonyms: TGF-beta 1, Tgfb-1, Tgfb, TGFbeta1, TGF-beta1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 21803
Homologene: 540
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: 2410098H20Rik, D5Bwg1524e, Rutbc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52850
Homologene: 64485
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, RAPT1, RAFT1, flat, Frap1, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Zfp532
Name: zinc finger protein 532
Synonyms: C530030I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 328977
Homologene: 138627
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107182
Homologene: 31978
Ankrd12
Name: ankyrin repeat domain 12
Synonyms: ANCO-2, GAC-1, 2900001A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106585
Homologene: 9059
Pkn2
Name: protein kinase N2
Synonyms: PRK2, 6030436C20Rik, Stk7, Prkcl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Wwox
Name: WW domain-containing oxidoreductase
Synonyms: WOX1, 9030416C10Rik, 5330426P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 80707
Homologene: 56334
Rbm27
Name: RNA binding motif protein 27
Synonyms: Psc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225432
VEGA: 18
Homologene: 35410
Sart3
Name: squamous cell carcinoma antigen recognized by T cells 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53890
Homologene: 40977
Polr3a
Name: polymerase (RNA) III (DNA directed) polypeptide A
Synonyms: RPC155, RPC1, 9330175N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218832
VEGA: 14
Homologene: 5124
Slc12a2
Name: solute carrier family 12, member 2
Synonyms: sodium/potassium/chloride cotransporters, mBSC2, Nkcc1, sy-ns
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20496
VEGA: 18
Homologene: 20283
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Slc45a4
Name: solute carrier family 45, member 4
Synonyms: 9330175B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 106068
Homologene: 69908
Arid1b
Name: AT rich interactive domain 1B (SWI-like)
Synonyms: B230217J03Rik, 9330189K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 239985
Homologene: 32344
Slc38a10
Name: solute carrier family 38, member 10
Synonyms: 1810073N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72055
Homologene: 41556
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Son
Name: Son DNA binding protein
Synonyms: 2900011L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 20658
Homologene: 10551
Wdr76
Name: WD repeat domain 76
Synonyms: 5830411K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241627
Homologene: 38573
Lrrfip1
Name: leucine rich repeat (in FLII) interacting protein 1
Synonyms: FLAP (FLI LRR associated protein), Fliiap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16978
HGNC: HGNC:6702
Homologene: 48301
Vamp2
Name: vesicle-associated membrane protein 2
Synonyms: Syb2, synaptobrevin II, Syb-2, sybII, synaptobrevin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22318
Homologene: 7591
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 404710
Homologene: 26400
Fgfr2
Name: fibroblast growth factor receptor 2
Synonyms: Bek, KGFRTr, Fgfr-7, Fgfr-2, Fgfr7, svs, Fgfr2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14183
HGNC: HGNC:3689
Homologene: 22566
Inpp5j
Name: inositol polyphosphate 5-phosphatase J
Synonyms: Pipp, Pib5pa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 170835
HGNC: HGNC:8956
Homologene: 8682
Grhl1
Name: grainyhead like transcription factor 1
Synonyms: p70 MGR, p61 MGR, LBP-32, Tcfcp2l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 195733
VEGA: 12
Homologene: 32219
Ncstn
Name: nicastrin
Synonyms: nicastrin, 9430068N19Rik, Nct, D1Dau13e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 59287
Homologene: 41029
Raph1
Name: Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Synonyms: C730009O10Rik, 9430025M21Rik, lamellipodin, Lpd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77300
Homologene: 71030
Usp8
Name: ubiquitin specific peptidase 8
Synonyms: Ubpy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 84092
Homologene: 3782
Eno4
Name: enolase 4
Synonyms: 6430537H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226265
Homologene: 18691
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: 4930516E05Rik, D330038K10Rik, 4930543C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Ccdc184
Name: coiled-coil domain containing 184
Synonyms: AI836003
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239650
VEGA: 15
Homologene: 18612
Nr1d1
Name: nuclear receptor subfamily 1, group D, member 1
Synonyms: rev-erbA(alpha), REV-ERBalpha, A530070C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217166
HGNC: HGNC:7962
Homologene: 23324
Hmcn2
Name: hemicentin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 665700
Homologene: 90772
Tarbp1
Name: TAR RNA binding protein 1
Synonyms: Gm17296
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 212728
Homologene: 38082
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Siglec1
Name: sialic acid binding Ig-like lectin 1, sialoadhesin
Synonyms: CD169, Siglec-1, Sn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20612
Homologene: 124458
Mfsd12
Name: major facilitator superfamily domain containing 12
Synonyms: Wdt1, F630110N24Rik, gr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73822
Homologene: 45488
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: 1700096C21Rik, sba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240411
Zbtb41
Name: zinc finger and BTB domain containing 41
Synonyms: 8430415N23Rik, 9830132G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226470
Homologene: 27795
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: A830096D10Rik, Bai3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Cfap53
Name: cilia and flagella associated protein 53
Synonyms: 4933415I03Rik, Ccdc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 74453
Homologene: 27056
Nlrc5
Name: NLR family, CARD domain containing 5
Synonyms: AI451557
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 434341
Homologene: 88935
Rabgap1l
Name: RAB GTPase activating protein 1-like
Synonyms: Hh1, 9630005B12Rik, 5830411O09Rik, 8430421H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 29809
Homologene: 8143
Snx25
Name: sorting nexin 25
Synonyms: LOC382008, SBBI31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102141
Homologene: 23744
Nanos3
Name: nanos C2HC-type zinc finger 3
Synonyms: nos3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244551
Homologene: 65856
Aox1
Name: aldehyde oxidase 1
Synonyms: retinal oxidase, Aox-1, Aox-2, Aox2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11761
HGNC: HGNC:553
Homologene: 68165
4921528O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Eya2
Name: EYA transcriptional coactivator and phosphatase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14049
HGNC: HGNC:3520
Homologene: 40711
Ankrd50
Name: ankyrin repeat domain 50
Synonyms: E430012K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99696
Homologene: 129859
Rbm46
Name: RNA binding motif protein 46
Synonyms: LOC329687, ENSMUSG00000033882
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 633285
Homologene: 17016
Etv3l
Name: ets variant 3-like
Synonyms: EG546801
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 546801
Homologene: 79096
Ptgfr
Name: prostaglandin F receptor
Synonyms: PGF, FP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19220
HGNC: HGNC:9600
Homologene: 741
Dcaf10
Name: DDB1 and CUL4 associated factor 10
Synonyms: Wdr32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242418
Homologene: 32581
Olfr273
Name: olfactory receptor 273
Synonyms: GA_x6K02T2N78B-7137430-7138383, MOR262-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 258821
Homologene: 73999
Mblac1
Name: metallo-beta-lactamase domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330216
Homologene: 45596
Cped1
Name: cadherin-like and PC-esterase domain containing 1
Synonyms: A430107O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 214642
Homologene: 57014
Tigd2
Name: tigger transposable element derived 2
Synonyms: 3632410O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68140
Homologene: 129603
Vmn1r67
Name: vomeronasal 1 receptor 67
Synonyms: V1re10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171263
Homologene: 128344
Urah
Name: urate (5-hydroxyiso-) hydrolase
Synonyms: 2810420C16Rik, HIU hydrolase, 1190003J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76974
Homologene: 45975
Klkb1
Name: kallikrein B, plasma 1
Synonyms: Kal-3, Kal3, Klk3, PSA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16621
HGNC: HGNC:6371
Homologene: 68097
Cd109
Name: CD109 antigen
Synonyms: Gov platelet alloantigens, 9930012E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235505
Homologene: 25183
Pls1
Name: plastin 1 (I-isoform)
Synonyms: I-fimbrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102502
HGNC: HGNC:9090
Homologene: 68270
Zbtb38
Name: zinc finger and BTB domain containing 38
Synonyms: A930014K01Rik, Zenon homolog, CIBZ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 245007
Homologene: 19529
H60b
Name: histocompatibility 60b
Synonyms: EG667281
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 667281
Homologene: 120804
Hal
Name: histidine ammonia lyase
Synonyms: histidase, Hsd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15109
HGNC: HGNC:4806
Homologene: 68229
Jmjd4
Name: jumonji domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 194952
Homologene: 11306
Nek9
Name: NIMA (never in mitosis gene a)-related expressed kinase 9
Synonyms: C130021H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217718
Homologene: 13222
Rhobtb3
Name: Rho-related BTB domain containing 3
Synonyms: 1700040C17Rik, 4930503C18Rik, 2610033K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 73296
Homologene: 8932
Synpo2l
Name: synaptopodin 2-like
Synonyms: 1110054M18Rik, Chap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68760
Homologene: 23499
Dppa2
Name: developmental pluripotency associated 2
Synonyms: 2410088E07Rik, ECAT15-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 73703
VEGA: 16
Homologene: 79572
Umodl1
Name: uromodulin-like 1
Synonyms: D17Ertd488e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 52020
VEGA: 17
Homologene: 45466
Cblif
Name: cobalamin binding intrinsic factor
Synonyms: Gif
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14603
HGNC: HGNC:4268
Homologene: 3773
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 25,101,482 bp
  • A to G, chromosome 1 at 58,073,212 bp
  • T to C, chromosome 1 at 60,500,605 bp
  • C to T, chromosome 1 at 91,093,647 bp
  • A to G, chromosome 1 at 139,441,834 bp
  • A to G, chromosome 1 at 160,627,369 bp
  • G to A, chromosome 1 at 163,062,487 bp
  • A to G, chromosome 1 at 172,069,961 bp
  • T to C, chromosome 2 at 31,428,237 bp
  • A to T, chromosome 2 at 69,465,627 bp
  • C to T, chromosome 2 at 121,535,419 bp
  • C to T, chromosome 2 at 126,737,889 bp
  • T to C, chromosome 2 at 131,073,397 bp
  • T to A, chromosome 2 at 165,716,011 bp
  • A to G, chromosome 3 at 38,483,049 bp
  • A to T, chromosome 3 at 82,864,230 bp
  • T to C, chromosome 3 at 87,554,968 bp
  • A to T, chromosome 3 at 88,115,949 bp
  • T to C, chromosome 3 at 142,810,827 bp
  • A to G, chromosome 3 at 151,835,091 bp
  • T to G, chromosome 4 at 45,374,262 bp
  • C to A, chromosome 4 at 52,856,196 bp
  • C to T, chromosome 4 at 148,538,881 bp
  • A to G, chromosome 5 at 113,268,750 bp
  • G to T, chromosome 5 at 113,755,397 bp
  • A to G, chromosome 5 at 138,194,465 bp
  • T to A, chromosome 6 at 22,119,602 bp
  • C to A, chromosome 6 at 59,211,384 bp
  • T to C, chromosome 6 at 146,232,291 bp
  • G to A, chromosome 7 at 10,446,866 bp
  • T to A, chromosome 7 at 25,694,354 bp
  • A to T, chromosome 7 at 130,180,488 bp
  • C to T, chromosome 7 at 140,836,752 bp
  • G to A, chromosome 8 at 45,289,196 bp
  • A to T, chromosome 8 at 46,105,109 bp
  • C to T, chromosome 8 at 84,176,134 bp
  • A to T, chromosome 8 at 94,474,656 bp
  • C to A, chromosome 8 at 114,439,830 bp
  • A to G, chromosome 8 at 126,447,530 bp
  • A to C, chromosome 9 at 78,680,021 bp
  • T to C, chromosome 9 at 95,787,063 bp
  • C to T, chromosome 9 at 96,687,676 bp
  • T to A, chromosome 10 at 22,287,231 bp
  • G to A, chromosome 10 at 81,362,814 bp
  • T to A, chromosome 10 at 93,498,991 bp
  • A to G, chromosome 11 at 3,501,417 bp
  • G to T, chromosome 11 at 55,310,039 bp
  • C to A, chromosome 11 at 59,453,952 bp
  • A to G, chromosome 11 at 69,089,759 bp
  • A to G, chromosome 11 at 98,770,478 bp
  • A to G, chromosome 11 at 101,524,977 bp
  • G to T, chromosome 11 at 120,110,312 bp
  • A to T, chromosome 12 at 24,612,151 bp
  • T to C, chromosome 12 at 85,301,899 bp
  • T to C, chromosome 13 at 11,595,784 bp
  • A to T, chromosome 13 at 75,902,245 bp
  • A to T, chromosome 14 at 20,666,272 bp
  • A to G, chromosome 14 at 24,469,255 bp
  • A to G, chromosome 15 at 73,605,436 bp
  • G to A, chromosome 15 at 98,168,460 bp
  • A to G, chromosome 16 at 48,317,398 bp
  • T to A, chromosome 16 at 91,658,977 bp
  • T to C, chromosome 17 at 5,314,034 bp
  • A to T, chromosome 17 at 30,968,477 bp
  • A to G, chromosome 17 at 65,984,803 bp
  • A to G, chromosome 18 at 42,298,464 bp
  • A to T, chromosome 18 at 57,915,522 bp
  • A to G, chromosome 18 at 65,644,333 bp
  • A to T, chromosome 18 at 74,299,173 bp
  • A to T, chromosome 18 at 77,408,778 bp
  • G to A, chromosome 19 at 11,749,756 bp
  • A to T, chromosome 19 at 16,676,140 bp
  • G to T, chromosome 19 at 37,003,524 bp
  • T to C, chromosome 19 at 58,964,538 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0048 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038342-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.