Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR0076Btlr/Mmmh
Stock Number:
038363-MU
Citation ID:
RRID:MMRRC_038363-MU
Other Names:
R0076 (G1), C57BL/6J-MtgxR0076Btlr
Major Collection:

Strain Information

Il4
Name: interleukin 4
Synonyms: Il-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16189
HGNC: HGNC:6014
Homologene: 491
Ccnd1
Name: cyclin D1
Synonyms: Cyl-1, bcl-1, PRAD1, cD1, CycD1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12443
HGNC: HGNC:1582
Homologene: 1334
Il17rd
Name: interleukin 17 receptor D
Synonyms: Sef, Sef-S, 2810004A10Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 171463
VEGA: 14
Homologene: 9717
Eif3g
Name: eukaryotic translation initiation factor 3, subunit G
Synonyms: TU-189B2, p44, 44kDa, D0Jmb4, Eif3s4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 53356
VEGA: 9
HGNC: HGNC:3274
Homologene: 2784
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: Gtar, 4933425K22Rik, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Zfp532
Name: zinc finger protein 532
Synonyms: C530030I18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 328977
Homologene: 138627
Tpx2
Name: TPX2, microtubule-associated
Synonyms: REPP86, DIL2, p100, 2610005B21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72119
HGNC: HGNC:1249
Homologene: 8107
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 24,237,497 bp
  • G to A, chromosome 1 at 120,227,730 bp
  • G to T, chromosome 1 at 173,874,447 bp
  • A to G, chromosome 2 at 66,714,037 bp
  • G to T, chromosome 2 at 75,661,696 bp
  • A to T, chromosome 2 at 79,343,289 bp
  • T to C, chromosome 2 at 148,442,136 bp
  • T to C, chromosome 2 at 152,893,683 bp
  • T to A, chromosome 2 at 163,727,603 bp
  • T to A, chromosome 3 at 22,189,785 bp
  • T to A, chromosome 3 at 104,353,132 bp
  • T to A, chromosome 3 at 133,160,746 bp
  • G to A, chromosome 4 at 11,609,480 bp
  • T to C, chromosome 4 at 75,947,039 bp
  • A to G, chromosome 4 at 104,945,464 bp
  • G to A, chromosome 4 at 124,991,397 bp
  • A to T, chromosome 4 at 145,164,694 bp
  • A to G, chromosome 5 at 25,186,072 bp
  • T to A, chromosome 5 at 77,326,561 bp
  • A to G, chromosome 5 at 87,254,221 bp
  • T to A, chromosome 5 at 90,244,406 bp
  • T to C, chromosome 5 at 101,817,840 bp
  • G to T, chromosome 5 at 114,408,217 bp
  • T to C, chromosome 6 at 21,952,171 bp
  • A to G, chromosome 6 at 135,227,226 bp
  • G to A, chromosome 7 at 25,353,958 bp
  • A to G, chromosome 7 at 45,678,891 bp
  • A to T, chromosome 7 at 99,685,501 bp
  • T to G, chromosome 7 at 120,373,685 bp
  • T to C, chromosome 7 at 126,410,862 bp
  • T to A, chromosome 7 at 141,016,007 bp
  • A to C, chromosome 7 at 144,939,665 bp
  • G to A, chromosome 8 at 11,218,713 bp
  • G to A, chromosome 8 at 23,190,705 bp
  • A to G, chromosome 8 at 37,545,442 bp
  • T to C, chromosome 8 at 107,054,133 bp
  • C to A, chromosome 8 at 121,098,739 bp
  • A to G, chromosome 9 at 20,897,753 bp
  • A to G, chromosome 9 at 44,830,059 bp
  • A to G, chromosome 9 at 104,324,218 bp
  • A to C, chromosome 9 at 106,911,486 bp
  • A to G, chromosome 10 at 13,535,594 bp
  • A to T, chromosome 10 at 125,228,070 bp
  • A to G, chromosome 10 at 130,394,193 bp
  • A to G, chromosome 11 at 32,603,722 bp
  • A to T, chromosome 11 at 53,613,914 bp
  • G to A, chromosome 11 at 70,008,100 bp
  • T to C, chromosome 11 at 84,148,636 bp
  • A to G, chromosome 11 at 91,575,909 bp
  • G to A, chromosome 11 at 93,490,597 bp
  • G to A, chromosome 11 at 97,857,655 bp
  • A to G, chromosome 11 at 120,485,442 bp
  • A to T, chromosome 11 at 120,792,808 bp
  • T to A, chromosome 12 at 13,324,336 bp
  • C to T, chromosome 12 at 30,104,023 bp
  • T to C, chromosome 13 at 53,113,074 bp
  • T to A, chromosome 14 at 27,094,854 bp
  • C to A, chromosome 14 at 46,817,455 bp
  • G to A, chromosome 15 at 75,947,209 bp
  • T to C, chromosome 15 at 76,191,414 bp
  • T to C, chromosome 15 at 76,451,140 bp
  • G to A, chromosome 15 at 100,587,836 bp
  • T to A, chromosome 16 at 13,715,518 bp
  • G to A, chromosome 16 at 38,302,684 bp
  • A to G, chromosome 17 at 6,064,937 bp
  • T to A, chromosome 17 at 53,947,877 bp
  • T to C, chromosome 18 at 37,478,359 bp
  • T to A, chromosome 18 at 65,685,627 bp
  • G to A, chromosome 18 at 71,321,046 bp
  • A to G, chromosome X at 13,678,274 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0076 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038363-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.