Strain Name:
C57BL/6J-MtgxR0092Btlr/Mmmh
Stock Number:
038379-MU
Citation ID:
RRID:MMRRC_038379-MU
Other Names:
R0092 (G1), C57BL/6J-MtgxR0092Btlr
Major Collection:

Gene Information

Gene Symbol: Kit [MGI:96677] (Mus musculus (mouse))
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: SCO5, CD117, belly-spot, Gsfsco1, Gsfsco5, Gsfsow3, Tr-kit, Dominant white spotting, SOW3, Steel Factor Receptor, c-KIT, SCO1
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Sbf2 [MGI:1921831] (Mus musculus (mouse))
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, Mtmr13, B430219L04Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Tnrc6b [MGI:2443730] (Mus musculus (mouse))
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Acadm [MGI:87867] (Mus musculus (mouse))
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Frem2 [MGI:2444465] (Mus musculus (mouse))
Name: Fras1 related extracellular matrix protein 2
Synonyms: ne, b2b1562Clo, 6030440P17Rik, my, 8430406N05Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Cacna1g [MGI:1201678] (Mus musculus (mouse))
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: Cav3.1d, a1G
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Lpp [MGI:2441849] (Mus musculus (mouse))
Name: LIM domain containing preferred translocation partner in lipoma
Synonyms: B130055L10Rik, 9430020K16Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 55,696,765 bp
  • A to G, chromosome 1 at 94,052,424 bp
  • G to A, chromosome 1 at 165,179,260 bp
  • T to C, chromosome 2 at 29,146,293 bp
  • A to G, chromosome 2 at 36,793,496 bp
  • T to C, chromosome 2 at 80,325,682 bp
  • G to T, chromosome 2 at 122,517,597 bp
  • C to T, chromosome 2 at 132,373,597 bp
  • A to G, chromosome 2 at 143,801,024 bp
  • A to G, chromosome 2 at 144,566,910 bp
  • T to C, chromosome 2 at 154,558,739 bp
  • A to G, chromosome 2 at 177,315,912 bp
  • G to A, chromosome 3 at 10,088,573 bp
  • A to G, chromosome 3 at 37,028,159 bp
  • A to G, chromosome 3 at 53,589,796 bp
  • G to A, chromosome 3 at 104,050,964 bp
  • C to T, chromosome 3 at 116,793,804 bp
  • A to T, chromosome 3 at 153,941,875 bp
  • C to A, chromosome 4 at 66,403,982 bp
  • T to A, chromosome 4 at 86,781,607 bp
  • A to G, chromosome 4 at 138,319,998 bp
  • C to T, chromosome 4 at 156,178,953 bp
  • T to A, chromosome 5 at 38,299,830 bp
  • T to A, chromosome 5 at 75,647,754 bp
  • C to A, chromosome 5 at 110,796,327 bp
  • T to C, chromosome 6 at 38,743,229 bp
  • T to G, chromosome 6 at 54,944,541 bp
  • T to A, chromosome 6 at 58,685,777 bp
  • T to C, chromosome 6 at 71,967,455 bp
  • T to C, chromosome 6 at 72,566,809 bp
  • A to G, chromosome 7 at 29,145,132 bp
  • A to G, chromosome 7 at 81,092,553 bp
  • T to C, chromosome 7 at 103,937,727 bp
  • T to C, chromosome 7 at 105,559,154 bp
  • T to C, chromosome 7 at 108,713,824 bp
  • T to C, chromosome 7 at 109,899,806 bp
  • T to A, chromosome 7 at 110,320,806 bp
  • A to G, chromosome 7 at 141,818,630 bp
  • T to A, chromosome 8 at 66,889,108 bp
  • T to C, chromosome 8 at 94,489,594 bp
  • A to G, chromosome 8 at 104,836,512 bp
  • TTCCAGATGTCC to TTCC, chromosome 9 at 15,090,603 bp
  • C to A, chromosome 9 at 63,145,995 bp
  • T to G, chromosome 9 at 64,948,752 bp
  • T to A, chromosome 9 at 88,370,285 bp
  • T to C, chromosome 9 at 103,489,305 bp
  • A to T, chromosome 9 at 104,090,341 bp
  • T to A, chromosome 9 at 106,013,314 bp
  • T to C, chromosome 9 at 110,085,010 bp
  • T to G, chromosome 9 at 120,952,863 bp
  • A to G, chromosome 10 at 17,842,755 bp
  • T to C, chromosome 10 at 79,491,964 bp
  • G to A, chromosome 10 at 81,561,220 bp
  • T to A, chromosome 10 at 82,879,802 bp
  • T to C, chromosome 10 at 120,089,808 bp
  • A to G, chromosome 10 at 129,608,221 bp
  • A to T, chromosome 11 at 20,094,308 bp
  • T to C, chromosome 11 at 51,629,592 bp
  • A to T, chromosome 11 at 59,051,247 bp
  • A to G, chromosome 11 at 68,492,803 bp
  • T to C, chromosome 11 at 69,642,146 bp
  • A to G, chromosome 11 at 69,779,351 bp
  • C to T, chromosome 11 at 94,457,264 bp
  • G to A, chromosome 11 at 100,121,432 bp
  • T to C, chromosome 11 at 103,046,493 bp
  • C to G, chromosome 11 at 115,862,986 bp
  • G to T, chromosome 11 at 115,979,124 bp
  • T to C, chromosome 12 at 3,496,313 bp
  • T to A, chromosome 12 at 99,954,989 bp
  • C to T, chromosome 13 at 49,662,447 bp
  • T to A, chromosome 13 at 91,741,565 bp
  • G to A, chromosome 14 at 16,409,263 bp
  • T to C, chromosome 14 at 30,940,863 bp
  • C to A, chromosome 14 at 31,191,453 bp
  • A to C, chromosome 14 at 31,271,609 bp
  • A to G, chromosome 14 at 55,677,945 bp
  • G to A, chromosome 14 at 66,053,887 bp
  • T to C, chromosome 15 at 76,183,743 bp
  • A to T, chromosome 15 at 80,918,528 bp
  • A to T, chromosome 15 at 85,067,724 bp
  • T to A, chromosome 15 at 100,202,265 bp
  • A to T, chromosome 16 at 22,826,094 bp
  • A to G, chromosome 16 at 24,761,602 bp
  • A to T, chromosome 16 at 29,625,594 bp
  • A to T, chromosome 16 at 31,447,562 bp
  • T to C, chromosome 16 at 45,667,596 bp
  • A to G, chromosome 16 at 94,365,462 bp
  • T to G, chromosome 17 at 37,700,805 bp
  • A to G, chromosome 17 at 47,467,515 bp
  • G to T, chromosome 17 at 56,018,088 bp
  • A to T, chromosome 17 at 57,250,798 bp
  • A to G, chromosome 17 at 64,659,084 bp
  • A to C, chromosome 17 at 68,425,703 bp
  • T to A, chromosome 17 at 69,286,750 bp
  • A to G, chromosome 18 at 36,689,224 bp
  • T to C, chromosome 19 at 39,183,780 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R0092 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    038379-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.