Strain Name:
C57BL/6J-MtgxR0217Btlr/Mmmh
Stock Number:
038466-MU
Citation ID:
RRID:MMRRC_038466-MU
Other Names:
R0217 (G1), C57BL/6J-MtgxR0217Btlr
Major Collection:

Gene Information

Pip5k1a
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
Synonyms: Pipk5a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 18720
HGNC: HGNC:8994
Homologene: 93492
Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: Rap, 4932417H02Rik, raptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74370
Homologene: 80210
Pank3
Name: pantothenate kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 211347
Homologene: 56999
Ctnnal1
Name: catenin (cadherin associated protein), alpha-like 1
Synonyms: ACRP, Catnal1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 54366
HGNC: HGNC:2512
Homologene: 2815
Cdk19
Name: cyclin-dependent kinase 19
Synonyms: Cdc2l6, 2700084L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 78334
VEGA: 10
Homologene: 22862
Zfp113
Name: zinc finger protein 113
Synonyms: 4732456B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 56314
Homologene: 50027
Wdr47
Name: WD repeat domain 47
Synonyms: 1810073M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99512
Homologene: 8984
Jak2
Name: Janus kinase 2
Synonyms: C81284
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 16452
VEGA: 19
HGNC: HGNC:6192
Homologene: 21033
Comp
Name: cartilage oligomeric matrix protein
Synonyms: thrombospondin-5, TSP5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12845
HGNC: HGNC:2227
Homologene: 74
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: 3-hydroxy-3-methylglutaryl-CoA reductase, Red, HMG-CoAR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Dsk9, Nf-1, Mhdadsk9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: D3Ertd775e, Lba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Sbno1
Name: strawberry notch 1
Synonyms: 9330180L10Rik, sno
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 243272
Homologene: 10055
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, Aspm, MCPH5, D330028K02Rik, Calmbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12316
Homologene: 7650
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: 2900087K15Rik, Rpo1-4, 3010014K16Rik, RPA194, mRPA1, 194kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 20019
Homologene: 7033
Itgb2
Name: integrin beta 2
Synonyms: Mac-1 beta, 2E6, Cd18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 16414
HGNC: HGNC:6155
Homologene: 20092
Ccnyl1
Name: cyclin Y-like 1
Synonyms: 9630037P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227210
Homologene: 65050
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: C130099L13Rik, 8430405D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 195018
Homologene: 9027
Bcl2
Name: B cell leukemia/lymphoma 2
Synonyms: C430015F12Rik, Bcl-2, D830018M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12043
HGNC: HGNC:990
Homologene: 527
Scaf4
Name: SR-related CTD-associated factor 4
Synonyms: Sfrs15, Sra4, Srsf15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 224432
VEGA: 16
Homologene: 16227
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Trpv4
Name: transient receptor potential cation channel, subfamily V, member 4
Synonyms: VROAC, VR-OAC, 0610033B08Rik, OTRPC4, Trp12, VRL-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 63873
Homologene: 11003
Stim1
Name: stromal interaction molecule 1
Synonyms: SIM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20866
Homologene: 20681
Cdc23
Name: CDC23 cell division cycle 23
Synonyms: D18Ertd243e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 52563
HGNC: HGNC:1724
Homologene: 3426
Pex19
Name: peroxisomal biogenesis factor 19
Synonyms: peroxisome biogenesis factor 19, Pxf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 19298
HGNC: HGNC:9713
Homologene: 134253
Foxk1
Name: forkhead box K1
Synonyms: A630048H08Rik, Mnf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 17425
Homologene: 82414
Actr3
Name: ARP3 actin-related protein 3
Synonyms: Arp3, 1200003A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74117
HGNC: HGNC:170
Homologene: 68483
Cyp20a1
Name: cytochrome P450, family 20, subfamily a, polypeptide 1
Synonyms: A930011N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 77951
Homologene: 18584
Zswim4
Name: zinc finger SWIM-type containing 4
Synonyms: E130119J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 212168
Homologene: 18183
Plxna2
Name: plexin A2
Synonyms: OCT, PlexA2, Plxn2, 2810428A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Man1a2
Name: mannosidase, alpha, class 1A, member 2
Synonyms: Man1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 17156
HGNC: HGNC:6822
Homologene: 55982
Stxbp1
Name: syntaxin binding protein 1
Synonyms: nsec1, Munc-18a, Unc18h, Sxtbp1, Munc18-1, Rb-sec1, N-sec1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20910
Homologene: 2382
Eci3
Name: enoyl-Coenzyme A delta isomerase 3
Synonyms: 1810022C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 69123
Homologene: 74357
Trpc7
Name: transient receptor potential cation channel, subfamily C, member 7
Synonyms: Trrp8, TRP7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 26946
Homologene: 22689
Adamts13
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13
Synonyms: vWF-CP mRNA for von Willebrand factor-cleaving, LOC279028
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 279028
HGNC: HGNC:1366
Homologene: 16372
Afap1l1
Name: actin filament associated protein 1-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 106877
Homologene: 18728
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Ccdc150
Name: coiled-coil domain containing 150
Synonyms: 4930511H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 78016
Homologene: 15814
Megf8
Name: multiple EGF-like-domains 8
Synonyms: m687Ddg, Egfl4, b2b288Clo, b2b1702Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Vmn2r82
Name: vomeronasal 2, receptor 82
Synonyms: EG624845
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 624845
Homologene: 83483
Zfp735
Name: zinc finger protein 735
Synonyms: 1700012C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76390
Homologene: 88945
Olfr390
Name: olfactory receptor 390
Synonyms: MOR135-26, GA_x6K02T2P1NL-3938806-3939741
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 258344
Homologene: 115483
Acte1
Name: actin, epsilon 1
Synonyms: Gm498, LOC244239
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 102636989
Homologene: 131918
Ppm1h
Name: protein phosphatase 1H (PP2C domain containing)
Synonyms: ARHCL1, C030002B11Rik, A430075L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 319468
Homologene: 18537
Tgtp1
Name: T cell specific GTPase 1
Synonyms: Tgtp, Mg21, Gtp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 21822
Homologene: 129991
Zfp661
Name: zinc finger protein 661
Synonyms: 2810405K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 72180
Homologene: 23275
Serpina3b
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3B
Synonyms: A030003A19Rik, alpha-1 antiproteinase, 6A1, antitrypsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 271047
HGNC: HGNC:16
Homologene: 120222
Olfr491
Name: olfactory receptor 491
Synonyms: GA_x6K02T2PBJ9-10646917-10647849, MOR204-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258731
Homologene: 128150
Gm12790
Name: predicted gene 12790
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
Slc39a10
Name: solute carrier family 39 (zinc transporter), member 10
Synonyms: Zip10, 2900042E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227059
Homologene: 34076
Fcnb
Name: ficolin B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 14134
Homologene: 1518
Plag1
Name: pleiomorphic adenoma gene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 56711
HGNC: HGNC:9045
Homologene: 1993
Hsdl2
Name: hydroxysteroid dehydrogenase like 2
Synonyms: 2610207I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 72479
Slc6a13
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 13
Synonyms: Gabt3, Gat2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14412
Homologene: 9592
Serpinb9d
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9d
Synonyms: ovalbumin, Spi9, AT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20726
HGNC: HGNC:8955
Homologene: 69093
Olfr746
Name: olfactory receptor 746
Synonyms: GA_x6K02T2PMLR-6372116-6373060, MOR106-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 258295
Homologene: 134081
Ptk2b
Name: PTK2 protein tyrosine kinase 2 beta
Synonyms: proline-rich tyrosine kinase 2, calcium-dependent tyrosine kinase, CAKbeta, related adhesion focal tyrosine kinase, cellular adhesion kinase beta, Raftk, E430023O05Rik, PYK2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 19229
HGNC: HGNC:9612
Homologene: 23001
Smco1
Name: single-pass membrane protein with coiled-coil domains 1
Synonyms: 2310010M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 69576
Homologene: 18842
Prelid2
Name: PRELI domain containing 2
Synonyms: 1700003A01Rik, C330008K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 77619
VEGA: 18
Homologene: 45749
Stxbp3-ps
Name: syntaxin-binding protein 3, pseudogene
Synonyms: Munc18c(L), Stxbp3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 619371
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 46,835,540 bp
  • T to G, chromosome 1 at 54,300,430 bp
  • C to A, chromosome 1 at 60,343,466 bp
  • T to A, chromosome 1 at 64,713,098 bp
  • G to A, chromosome 1 at 106,712,562 bp
  • A to G, chromosome 1 at 125,407,413 bp
  • T to A, chromosome 1 at 139,457,880 bp
  • T to A, chromosome 1 at 172,126,743 bp
  • T to A, chromosome 1 at 194,644,598 bp
  • A to G, chromosome 2 at 26,996,921 bp
  • T to C, chromosome 2 at 28,079,677 bp
  • T to C, chromosome 2 at 32,801,870 bp
  • C to T, chromosome 2 at 127,577,291 bp
  • T to C, chromosome 3 at 86,642,722 bp
  • A to G, chromosome 3 at 95,073,991 bp
  • A to G, chromosome 3 at 100,617,037 bp
  • T to A, chromosome 3 at 108,637,020 bp
  • A to C, chromosome 4 at 3,904,379 bp
  • T to C, chromosome 4 at 56,813,230 bp
  • A to G, chromosome 4 at 59,597,311 bp
  • T to C, chromosome 4 at 101,968,034 bp
  • C to A, chromosome 5 at 114,634,661 bp
  • T to C, chromosome 5 at 124,404,324 bp
  • T to C, chromosome 5 at 138,150,691 bp
  • T to C, chromosome 5 at 142,401,894 bp
  • T to C, chromosome 6 at 71,963,703 bp
  • T to A, chromosome 6 at 85,622,930 bp
  • T to A, chromosome 6 at 121,324,320 bp
  • T to A, chromosome 7 at 25,364,079 bp
  • T to A, chromosome 7 at 102,435,800 bp
  • C to A, chromosome 7 at 108,317,298 bp
  • T to C, chromosome 7 at 143,894,219 bp
  • A to C, chromosome 8 at 70,378,908 bp
  • A to G, chromosome 8 at 84,212,664 bp
  • A to T, chromosome 9 at 63,256,975 bp
  • C to T, chromosome 10 at 40,476,258 bp
  • G to T, chromosome 10 at 77,548,536 bp
  • A to G, chromosome 10 at 79,378,800 bp
  • T to A, chromosome 10 at 122,920,735 bp
  • T to A, chromosome 11 at 35,777,728 bp
  • A to C, chromosome 11 at 48,987,319 bp
  • T to A, chromosome 11 at 72,889,068 bp
  • T to A, chromosome 11 at 73,711,286 bp
  • T to A, chromosome 11 at 73,787,388 bp
  • A to T, chromosome 11 at 79,428,574 bp
  • T to C, chromosome 11 at 119,894,912 bp
  • C to T, chromosome 12 at 104,130,727 bp
  • C to A, chromosome 13 at 33,198,022 bp
  • A to C, chromosome 13 at 34,948,089 bp
  • C to T, chromosome 13 at 56,789,768 bp
  • A to C, chromosome 13 at 96,651,980 bp
  • A to G, chromosome 14 at 50,654,095 bp
  • T to C, chromosome 14 at 66,156,381 bp
  • G to T, chromosome 16 at 32,273,781 bp
  • T to C, chromosome 16 at 90,242,682 bp
  • T to C, chromosome 18 at 34,651,665 bp
  • A to T, chromosome 18 at 41,935,252 bp
  • C to G, chromosome 18 at 44,519,516 bp
  • A to G, chromosome 18 at 61,746,869 bp
  • T to G, chromosome 19 at 9,559,132 bp
  • T to A, chromosome 19 at 29,296,650 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0217 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038466-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.