Strain Name:
C57BL/6J-MtgxR0227Btlr/Mmmh
Stock Number:
038472-MU
Citation ID:
RRID:MMRRC_038472-MU
Other Names:
R0227 (G1), C57BL/6J-MtgxR0227Btlr
Major Collection:

Gene Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Del1, Lpk, M100856, Col2, Rgsc856, Col2a, Col2a-1, M100413, Rgsc413
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Atn1
Name: atrophin 1
Synonyms: Drpla, atrophin-1, Atr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 13498
HGNC: HGNC:3033
Homologene: 1461
Ess2
Name: ess-2 splicing factor
Synonyms: Dgcr14, Es2el, Dgsi, ES2, D16H22S1269E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 27886
VEGA: 16
Homologene: 11184
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, Acf7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 11426
Homologene: 136191
Ankrd12
Name: ankyrin repeat domain 12
Synonyms: ANCO-2, 2900001A12Rik, GAC-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 106585
Homologene: 9059
Myo9b
Name: myosin IXb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 17925
HGNC: HGNC:7609
Homologene: 3058
Pdcd11
Name: programmed cell death 11
Synonyms: ALG-4, 1110021I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 18572
VEGA: 19
Homologene: 74968
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 21819
Homologene: 2430
Son
Name: Son DNA binding protein
Synonyms: 2900011L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 20658
Homologene: 10551
Ice2
Name: interactor of little elongation complex ELL subunit 2
Synonyms: Narg2, B230343B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 93697
Homologene: 11618
Ap4m1
Name: adaptor-related protein complex AP-4, mu 1
Synonyms: 4930443L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11781
HGNC: HGNC:574
Homologene: 3467
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: p600, A930005E13Rik, 1810009A16Rik, LOC381562, D930005K06Rik, Zubr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69116
Homologene: 10804
Crim1
Name: cysteine rich transmembrane BMP regulator 1 (chordin like)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 50766
VEGA: 17
HGNC: HGNC:2359
Homologene: 9510
Synrg
Name: synergin, gamma
Synonyms: L71-5, Ap1gbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217030
HGNC: HGNC:557
Homologene: 105680
Nudt9
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 9
Synonyms: 1190002C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 74167
HGNC: HGNC:8056
Homologene: 11435
Raph1
Name: Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Synonyms: Lpd, C730009O10Rik, lamellipodin, 9430025M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 77300
Homologene: 71030
Rabgef1
Name: RAB guanine nucleotide exchange factor (GEF) 1
Synonyms: Ras negative regulator Rabex-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 56715
Homologene: 8720
Zic5
Name: zinc finger protein of the cerebellum 5
Synonyms: 1700049L20Rik, odd-paired related, Opr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 65100
Homologene: 11301
Jag1
Name: jagged 1
Synonyms: Ozz, Serrate-1, Gsfabe2, Htu, ABE2, Headturner
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16449
HGNC: HGNC:6188
Homologene: 180
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 94109
Homologene: 69536
Ceacam18
Name: carcinoembryonic antigen-related cell adhesion molecule 18
Synonyms: 2010110O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72431
Homologene: 48111
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Stk10
Name: serine/threonine kinase 10
Synonyms: Lok, Gek1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20868
Homologene: 38122
D430041D05Rik
Name: RIKEN cDNA D430041D05 gene
Synonyms: G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241589
Homologene: 115928
9430038I01Rik
Name: RIKEN cDNA 9430038I01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77252
HGNC: null
Homologene: 130777
Myo1c
Name: myosin IC
Synonyms: C80397, myosin-Ibeta, mm1beta, myr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 17913
HGNC: HGNC:7597
Homologene: 32046
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 241116
Homologene: 28093
Synpo2
Name: synaptopodin 2
Synonyms: 2310068J10Rik, 1110069I04Rik, Myo, myopodin, 9530006G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 118449
Homologene: 15400
Slc34a2
Name: solute carrier family 34 (sodium phosphate), member 2
Synonyms: Npt2b, D5Ertd227e, type IIb Na/Picotransporter, NaPi-2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 20531
Homologene: 2297
Bcl9l
Name: B cell CLL/lymphoma 9-like
Synonyms: DLNB11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 80288
VEGA: 9
Homologene: 65615
Sp100
Name: nuclear antigen Sp100
Synonyms: A430075G10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20684
Homologene: 86761
Olfr948
Name: olfactory receptor 948
Synonyms: MOR171-45, MOR171-51, GA_x6K02T2PVTD-33016899-33015934
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 257912
VEGA: 9
HGNC: null
Homologene: 79377
Vmn1r63
Name: vomeronasal 1 receptor 63
Synonyms: V1R1, V1rd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 81017
HGNC: null
Homologene: 41799
Vmn2r92
Name: vomeronasal 2, receptor 92
Synonyms: EG627111
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627111
HGNC: null
Homologene: 115024
Raly
Name: hnRNP-associated with lethal yellow
Synonyms: Merc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19383
Homologene: 7216
Gtf3a
Name: general transcription factor III A
Synonyms: 5330403M05Rik, 2610111I01Rik, 2010015D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 66596
HGNC: HGNC:4662
Homologene: 55630
Tspan33
Name: tetraspanin 33
Synonyms: Penumbra, 1300010A20Rik, Pen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232670
Homologene: 8213
Mogat2
Name: monoacylglycerol O-acyltransferase 2
Synonyms: Mgat2, DGAT2L5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233549
Homologene: 57020
Vmn1r210
Name: vomeronasal 1 receptor 210
Synonyms: V1rh10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 171269
HGNC: null
Homologene: 110880
Spock1
Name: sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1
Synonyms: testican 1, Ticn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20745
Homologene: 80193
Gm3486
Name: predicted gene 3486
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: null
HGNC: null
Homologene: null
Tafa5
Name: TAFA chemokine like family member 5
Synonyms: Tara-5, Fam19a5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 106014
VEGA: 15
Homologene: 22888
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,525,977 bp
  • C to T, chromosome 1 at 74,931,958 bp
  • T to C, chromosome 1 at 85,673,613 bp
  • G to T, chromosome 2 at 104,205,200 bp
  • A to G, chromosome 2 at 137,115,618 bp
  • A to G, chromosome 2 at 154,865,921 bp
  • A to G, chromosome 3 at 123,113,793 bp
  • T to C, chromosome 4 at 123,399,391 bp
  • T to C, chromosome 4 at 139,431,649 bp
  • T to C, chromosome 5 at 53,069,626 bp
  • T to C, chromosome 5 at 104,061,675 bp
  • T to C, chromosome 5 at 130,190,990 bp
  • T to C, chromosome 5 at 138,176,276 bp
  • C to A, chromosome 5 at 146,955,389 bp
  • G to A, chromosome 6 at 29,713,478 bp
  • A to T, chromosome 6 at 124,746,930 bp
  • C to T, chromosome 7 at 5,802,742 bp
  • G to T, chromosome 7 at 43,639,391 bp
  • T to A, chromosome 7 at 99,223,132 bp
  • A to T, chromosome 7 at 137,387,709 bp
  • G to A, chromosome 7 at 141,639,559 bp
  • A to G, chromosome 8 at 16,391,822 bp
  • A to G, chromosome 8 at 71,344,162 bp
  • A to G, chromosome 9 at 39,318,678 bp
  • A to G, chromosome 9 at 44,505,236 bp
  • A to G, chromosome 9 at 69,412,228 bp
  • G to T, chromosome 11 at 32,617,859 bp
  • A to T, chromosome 11 at 75,658,694 bp
  • T to A, chromosome 11 at 84,009,432 bp
  • C to T, chromosome 13 at 22,827,391 bp
  • C to T, chromosome 13 at 41,105,856 bp
  • T to C, chromosome 13 at 57,440,477 bp
  • A to T, chromosome 14 at 41,484,561 bp
  • A to G, chromosome 14 at 122,459,661 bp
  • C to A, chromosome 15 at 66,698,446 bp
  • T to C, chromosome 15 at 87,720,490 bp
  • C to T, chromosome 15 at 97,976,755 bp
  • C to T, chromosome 16 at 17,902,271 bp
  • T to A, chromosome 16 at 91,656,873 bp
  • C to T, chromosome 17 at 18,167,957 bp
  • T to A, chromosome 17 at 65,987,227 bp
  • A to G, chromosome 17 at 78,344,509 bp
  • AGAGGAGGAGGAGGAGGAGGAGGAG to AGAGGAGGAGGAGGAGGAGGAG, chromosome 19 at 47,113,437 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0227 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038472-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.