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Strain Name:
C57BL/6J-MtgxR0245Btlr/Mmmh
Stock Number:
038483-MU
Citation ID:
RRID:MMRRC_038483-MU
Other Names:
R0245 (G1), C57BL/6J-MtgxR0245Btlr
Major Collection:

Strain Information

Itgb8
Name: integrin beta 8
Synonyms: 4832412O06Rik, D630049N15
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320910
HGNC: HGNC:6163
Homologene: 18567
Marchf4
Name: membrane associated ring-CH-type finger 4
Synonyms: March4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381270
Homologene: 66199
Slc17a5
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235504
Homologene: 56571
Got1
Name: glutamic-oxaloacetic transaminase 1, soluble
Synonyms: cAspAT, cytosolic aspartate aminotransferase, Got-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14718
HGNC: HGNC:4432
Homologene: 1571
Eps8
Name: epidermal growth factor receptor pathway substrate 8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13860
HGNC: HGNC:3420
Homologene: 3272
Rnf111
Name: ring finger 111
Synonyms: Arkadia
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 93836
VEGA: 9
Homologene: 9741
Adra2a
Name: adrenergic receptor, alpha 2a
Synonyms: alpha2A, alpha2A-adrenergic receptor, Adra-2a, Adra-2, alpha2A-AR, alpha(2A)AR
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 11551
VEGA: 19
HGNC: HGNC:281
Homologene: 47944
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,540,400 bp
  • A to T, chromosome 1 at 43,075,592 bp
  • T to C, chromosome 1 at 53,501,526 bp
  • A to T, chromosome 1 at 72,534,781 bp
  • T to A, chromosome 1 at 173,215,957 bp
  • A to G, chromosome 2 at 28,834,964 bp
  • A to G, chromosome 2 at 39,035,259 bp
  • C to A, chromosome 2 at 88,909,875 bp
  • A to G, chromosome 2 at 111,780,335 bp
  • A to G, chromosome 2 at 126,035,955 bp
  • C to T, chromosome 2 at 155,391,211 bp
  • C to T, chromosome 2 at 178,481,632 bp
  • T to C, chromosome 2 at 181,202,491 bp
  • A to T, chromosome 3 at 20,012,796 bp
  • A to G, chromosome 3 at 82,108,787 bp
  • A to G, chromosome 3 at 89,093,771 bp
  • T to C, chromosome 4 at 65,794,558 bp
  • T to C, chromosome 4 at 99,055,349 bp
  • T to C, chromosome 4 at 118,175,689 bp
  • T to A, chromosome 4 at 130,337,154 bp
  • T to C, chromosome 4 at 137,514,722 bp
  • C to A, chromosome 5 at 24,413,838 bp
  • A to G, chromosome 5 at 35,030,080 bp
  • G to A, chromosome 5 at 91,074,800 bp
  • A to G, chromosome 5 at 112,337,528 bp
  • T to C, chromosome 5 at 112,475,566 bp
  • G to A, chromosome 5 at 117,252,679 bp
  • A to G, chromosome 5 at 141,954,958 bp
  • A to G, chromosome 5 at 145,126,860 bp
  • C to A, chromosome 5 at 147,306,473 bp
  • G to T, chromosome 6 at 39,027,768 bp
  • A to G, chromosome 6 at 55,898,007 bp
  • A to G, chromosome 6 at 90,344,197 bp
  • T to C, chromosome 6 at 118,604,454 bp
  • A to T, chromosome 6 at 119,308,721 bp
  • T to C, chromosome 6 at 137,479,128 bp
  • T to C, chromosome 6 at 146,618,609 bp
  • C to T, chromosome 7 at 3,611,574 bp
  • T to A, chromosome 7 at 27,200,603 bp
  • A to C, chromosome 7 at 84,595,498 bp
  • T to C, chromosome 8 at 70,131,578 bp
  • T to C, chromosome 8 at 71,465,075 bp
  • A to G, chromosome 8 at 124,651,429 bp
  • C to A, chromosome 9 at 19,537,112 bp
  • C to T, chromosome 9 at 70,453,831 bp
  • A to G, chromosome 9 at 78,540,924 bp
  • T to A, chromosome 9 at 109,436,168 bp
  • T to A, chromosome 10 at 14,458,066 bp
  • A to G, chromosome 10 at 18,525,108 bp
  • A to T, chromosome 10 at 80,647,806 bp
  • C to A, chromosome 10 at 110,774,795 bp
  • T to A, chromosome 11 at 98,625,241 bp
  • T to C, chromosome 11 at 99,010,096 bp
  • C to T, chromosome 11 at 103,209,095 bp
  • T to C, chromosome 12 at 16,696,456 bp
  • A to T, chromosome 12 at 26,469,518 bp
  • C to T, chromosome 12 at 73,975,032 bp
  • T to A, chromosome 12 at 119,190,555 bp
  • A to G, chromosome 13 at 42,164,290 bp
  • A to T, chromosome 13 at 67,207,835 bp
  • A to G, chromosome 14 at 56,438,609 bp
  • G to T, chromosome 14 at 57,630,240 bp
  • A to T, chromosome 15 at 36,253,032 bp
  • A to T, chromosome 16 at 23,012,181 bp
  • A to T, chromosome 16 at 58,872,866 bp
  • G to T, chromosome 17 at 6,051,756 bp
  • T to C, chromosome 17 at 45,036,775 bp
  • C to T, chromosome 17 at 56,606,025 bp
  • A to T, chromosome 19 at 43,504,507 bp
  • G to C, chromosome 19 at 54,047,409 bp
  • T to C, chromosome 19 at 58,300,554 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0245 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038483-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.