Strain Name:
C57BL/6J-MtgxR0294Btlr/Mmmh
Stock Number:
038511-MU
Citation ID:
RRID:MMRRC_038511-MU
Other Names:
R0294 (G1), C57BL/6J-MtgxR0294Btlr
Major Collection:

Strain Information

Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228852
Homologene: 9194
Senp6
Name: SUMO/sentrin specific peptidase 6
Synonyms: E130319N12Rik, 2810017C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 215351
Homologene: 9196
Aplf
Name: aprataxin and PNKP like factor
Synonyms: 2010301N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 72103
Homologene: 49753
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12695
Homologene: 72199
Nln
Name: neurolysin (metallopeptidase M3 family)
Synonyms: 4930472G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75805
VEGA: 13
Homologene: 69315
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208628
Homologene: 32227
Bub3
Name: BUB3 mitotic checkpoint protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12237
HGNC: HGNC:1151
Homologene: 3470
Brwd1
Name: bromodomain and WD repeat domain containing 1
Synonyms: 5330419I02Rik, D530019K20Rik, G1-403-16, Wdr9, repro5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 93871
Homologene: 23130
Marf1
Name: meiosis regulator and mRNA stability 1
Synonyms: 4921513D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 223989
VEGA: 16
Homologene: 40967
Aqr
Name: aquarius
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11834
Homologene: 7629
Pphln1
Name: periphilin 1
Synonyms: 1600022A19Rik, 1110063K05Rik, CR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223828
VEGA: 15
Homologene: 9533
Map3k14
Name: mitogen-activated protein kinase kinase kinase 14
Synonyms: Nik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53859
HGNC: HGNC:6853
Homologene: 2940
Nbeal2
Name: neurobeachin-like 2
Synonyms: 1110014F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235627
Homologene: 86422
Lgr6
Name: leucine-rich repeat-containing G protein-coupled receptor 6
Synonyms: A530037C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329252
Homologene: 49680
Alas1
Name: aminolevulinic acid synthase 1
Synonyms: 5-aminolevulinate synthase, succinyl-CoA: glycine C-succinyl transferase, Alas-1, ALAS-N
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11655
HGNC: HGNC:396
Homologene: 55478
Metap1d
Name: methionyl aminopeptidase type 1D (mitochondrial)
Synonyms: 2310066F24Rik, 3110033D18Rik, Metapl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66559
Homologene: 11987
Srpr
Name: signal recognition particle receptor ('docking protein')
Synonyms: 1300011P19Rik, D11Mgi27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67398
Homologene: 2364
Ints7
Name: integrator complex subunit 7
Synonyms: 5930412E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77065
Homologene: 9136
Cblb
Name: Casitas B-lineage lymphoma b
Synonyms: Cbl-b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208650
VEGA: 16
HGNC: HGNC:1542
Homologene: 15856
Ehbp1
Name: EH domain binding protein 1
Synonyms: Flj21950, KIAA0903-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216565
Homologene: 22880
Dock8
Name: dedicator of cytokinesis 8
Synonyms: 1200017A24Rik, 5830472H07Rik, A130095G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 76088
VEGA: 19
Homologene: 52414
Chst1
Name: carbohydrate sulfotransferase 1
Synonyms: KSGAL6ST, C6ST, 2610008E20Rik, GST-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76969
HGNC: HGNC:1969
Homologene: 2711
Abca13
Name: ATP-binding cassette, sub-family A (ABC1), member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268379
Homologene: 27991
Foxp2
Name: forkhead box P2
Synonyms: 2810043D05Rik, D0Kist7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 114142
Homologene: 134404
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329650
Homologene: 43143
Nlgn1
Name: neuroligin 1
Synonyms: 6330415N05Rik, NL1, Nlg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 192167
Homologene: 56690
Egfem1
Name: EGF-like and EMI domain containing 1
Synonyms: 6130401L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 75740
Homologene: 135950
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Mroh4
Name: maestro heat-like repeat family member 4
Synonyms: 1700016M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69439
Homologene: 72545
Aadat
Name: aminoadipate aminotransferase
Synonyms: mKat-2, Kat2, KATII, Kyat2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23923
Homologene: 56540
Aknad1
Name: AKNA domain containing 1
Synonyms: 4921525H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329738
Homologene: 51892
Il5ra
Name: interleukin 5 receptor, alpha
Synonyms: IL-5 receptor alpha chain, CD125, Il5r, CDw125
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16192
HGNC: HGNC:6017
Homologene: 473
Slc17a3
Name: solute carrier family 17 (sodium phosphate), member 3
Synonyms: Npt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105355
Homologene: 21319
Crybg1
Name: crystallin beta-gamma domain containing 1
Synonyms: Aim1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11630
HGNC: HGNC:356
Homologene: 18168
Kcnt1
Name: potassium channel, subfamily T, member 1
Synonyms: Slack, slo2, C030030G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227632
Homologene: 11055
Plbd2
Name: phospholipase B domain containing 2
Synonyms: 66.3 kDa, p76, 1300012G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71772
Homologene: 41528
Otogl
Name: otogelin-like
Synonyms: Gm6924
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 628870
Homologene: 46008
Nnt
Name: nicotinamide nucleotide transhydrogenase
Synonyms: 4930423F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18115
HGNC: HGNC:7863
Or2z8
Name: olfactory receptor family 2 subfamily Z member 8
Synonyms: GA_x6K02T2NUPS-191522-192466, MOR282-1, Olfr372
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 404315
Homologene: 17287
Ces2h
Name: carboxylesterase 2H
Synonyms: Gm5744
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 436059
HGNC: HGNC:1864
Homologene: 128645
Atp11a
Name: ATPase, class VI, type 11A
Synonyms: Ih, 4930558F19Rik, 9130422H11Rik, LOC100045280
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 50770
Homologene: 75050
Dmrt2
Name: doublesex and mab-3 related transcription factor 2
Synonyms: Terra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226049
VEGA: 19
HGNC: HGNC:2935
Homologene: 17111
Cyp2d22
Name: cytochrome P450, family 2, subfamily d, polypeptide 22
Synonyms: 2D22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 56448
VEGA: 15
Homologene: 75003
Hsdl2
Name: hydroxysteroid dehydrogenase like 2
Synonyms: 2610207I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72479
Slc5a4b
Name: solute carrier family 5 (neutral amino acid transporters, system A), member 4b
Synonyms: 2010104G07Rik, pSGLT2, SAAT1, SGLT3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64454
Homologene: 56968
Cfh
Name: complement component factor h
Synonyms: Sas-1, Mud-1, Sas1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12628
Homologene: 20086
Gm597
Name: predicted gene 597
Synonyms: LOC210962
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210962
Prss40
Name: protease, serine 40
Synonyms: Tesp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21756
Homologene: 69043
Sphkap
Name: SPHK1 interactor, AKAP domain containing
Synonyms: A930009L15Rik, 4930544G21Rik, SKIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77629
Homologene: 18172
Cntnap5a
Name: contactin associated protein-like 5A
Synonyms: Caspr5-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 636808
Homologene: 43974
4930527J03Rik
Name: RIKEN cDNA 4930527J03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75164
Or9g4
Name: olfactory receptor family 9 subfamily G member 4
Synonyms: GA_x6K02T2Q125-47154544-47153606, MOR213-4, Olfr1006
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258563
Homologene: 17295
Mgst2
Name: microsomal glutathione S-transferase 2
Synonyms: MGST-II, GST2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 211666
HGNC: HGNC:7063
Homologene: 1811
Actl7b
Name: actin-like 7b
Synonyms: Tact1, t-actin 1, ENSMUSG00000070980
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11471
HGNC: HGNC:162
Homologene: 21330
Lexm
Name: lymphocyte expansion molecule
Synonyms: BC055111
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242602
Homologene: 17630
BC051076
Name: cDNA sequence BC051076
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243090
Slc13a1
Name: solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms: NaSi-1, Nas1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 55961
Homologene: 31893
H2aj
Name: H2J.A histone
Synonyms: H2afj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232440
Homologene: 128575
Or52s6
Name: olfactory receptor family 52 subfamily S member 6
Synonyms: GA_x6K02T2PBJ9-6164792-6163848, MOR202-22P, MOR24-5, Olfr605
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258156
Homologene: 79414
Or51b17
Name: olfactory receptor family 51 subfamily B member 17
Synonyms: 5'[b]2, MOR1-2, GA_x6K02T2PBJ9-6648196-6649143, Olfr64
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18366
Homologene: 56593
Or5p78
Name: olfactory receptor family 5 subfamily P member 78
Synonyms: GA_x6K02T2PBJ9-10942040-10942984, MOR204-23, Olfr506
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258215
Homologene: 133672
Slc22a18
Name: solute carrier family 22 (organic cation transporter), member 18
Synonyms: TSSC5, IMPT1, BWSCR1A, Orctl2, Impt1, p45-BWR1A, BWR1A, Slc22a1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18400
Homologene: 1918
Adam29
Name: a disintegrin and metallopeptidase domain 29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244486
HGNC: HGNC:207
Homologene: 8607
Gins3
Name: GINS complex subunit 3 (Psf3 homolog)
Synonyms: 2700085M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 78833
Homologene: 41496
Shank3
Name: SH3 and multiple ankyrin repeat domains 3
Synonyms: ProSAP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 58234
Homologene: 75163
Krt8
Name: keratin 8
Synonyms: K8, EndoA, cytokeratin-8, cytokeratin8, cytokeratin 8, Krt-2.8, Card2, Krt2-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16691
VEGA: 15
HGNC: HGNC:6446
Homologene: 55643
Trmt10c
Name: tRNA methyltransferase 10C, mitochondrial RNase P subunit
Synonyms: 1300018J16Rik, D16Ertd454e, Rg9mtd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 52575
Homologene: 9858
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 28,778,663 bp
  • T to G, chromosome 1 at 34,556,081 bp
  • T to A, chromosome 1 at 83,278,245 bp
  • T to A, chromosome 1 at 115,915,316 bp
  • A to G, chromosome 1 at 134,987,891 bp
  • T to A, chromosome 1 at 135,105,061 bp
  • A to G, chromosome 1 at 140,183,261 bp
  • ACCC to ACC, chromosome 1 at 178,276,503 bp
  • A to G, chromosome 1 at 191,611,891 bp
  • A to G, chromosome 2 at 25,888,110 bp
  • T to A, chromosome 2 at 71,522,545 bp
  • A to G, chromosome 2 at 85,674,716 bp
  • G to T, chromosome 2 at 92,613,642 bp
  • T to A, chromosome 2 at 114,170,071 bp
  • C to T, chromosome 2 at 158,746,603 bp
  • C to A, chromosome 3 at 26,133,476 bp
  • A to G, chromosome 3 at 29,690,121 bp
  • A to G, chromosome 3 at 51,681,830 bp
  • T to C, chromosome 3 at 59,075,829 bp
  • A to G, chromosome 3 at 108,775,192 bp
  • A to T, chromosome 4 at 56,740,848 bp
  • T to A, chromosome 4 at 59,601,408 bp
  • G to T, chromosome 4 at 98,497,048 bp
  • T to C, chromosome 4 at 106,613,164 bp
  • C to A, chromosome 5 at 87,964,417 bp
  • A to G, chromosome 5 at 120,487,449 bp
  • T to A, chromosome 5 at 123,802,207 bp
  • C to A, chromosome 6 at 15,376,774 bp
  • T to A, chromosome 6 at 24,090,780 bp
  • A to G, chromosome 6 at 87,646,245 bp
  • A to T, chromosome 6 at 106,712,401 bp
  • C to G, chromosome 6 at 136,808,604 bp
  • G to A, chromosome 7 at 103,443,084 bp
  • A to T, chromosome 7 at 103,892,930 bp
  • A to T, chromosome 7 at 108,613,150 bp
  • A to G, chromosome 7 at 131,568,224 bp
  • G to A, chromosome 7 at 143,492,841 bp
  • G to A, chromosome 8 at 12,827,524 bp
  • C to A, chromosome 8 at 55,873,276 bp
  • A to G, chromosome 8 at 60,534,608 bp
  • C to T, chromosome 8 at 72,058,400 bp
  • T to C, chromosome 8 at 95,637,919 bp
  • T to A, chromosome 8 at 105,016,604 bp
  • T to A, chromosome 9 at 35,215,515 bp
  • A to G, chromosome 9 at 80,113,725 bp
  • T to A, chromosome 9 at 106,241,256 bp
  • T to G, chromosome 9 at 110,632,859 bp
  • A to T, chromosome 10 at 11,080,399 bp
  • A to T, chromosome 10 at 43,986,376 bp
  • A to G, chromosome 10 at 76,081,327 bp
  • C to T, chromosome 10 at 107,777,228 bp
  • A to G, chromosome 11 at 9,269,122 bp
  • T to C, chromosome 11 at 22,095,427 bp
  • T to C, chromosome 11 at 103,227,137 bp
  • C to T, chromosome 13 at 23,855,858 bp
  • C to T, chromosome 13 at 104,052,579 bp
  • T to A, chromosome 13 at 119,336,267 bp
  • T to G, chromosome 13 at 119,338,417 bp
  • A to T, chromosome 15 at 44,560,435 bp
  • T to C, chromosome 15 at 74,606,149 bp
  • A to G, chromosome 15 at 82,374,445 bp
  • A to G, chromosome 15 at 89,532,098 bp
  • T to C, chromosome 15 at 93,420,290 bp
  • T to C, chromosome 15 at 101,997,842 bp
  • C to T, chromosome 16 at 14,142,534 bp
  • T to G, chromosome 16 at 52,135,824 bp
  • A to T, chromosome 16 at 56,034,877 bp
  • T to A, chromosome 16 at 96,056,107 bp
  • T to C, chromosome 19 at 25,188,350 bp
  • C to T, chromosome 19 at 25,678,071 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0294 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038511-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.