Strain Name:
C57BL/6J-MtgxR0325Btlr/Mmmh
Stock Number:
038535-MU
Citation ID:
RRID:MMRRC_038535-MU
Other Names:
R0325 (G1), C57BL/6J-MtgxR0325Btlr
Major Collection:

Gene Information

Gene Symbol: Hdgfl2 [MGI:1194492] (Mus musculus (mouse))
Name: HDGF like 2
Synonyms: Hdgfrp2, HRP-2
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Shprh [MGI:1917581] (Mus musculus (mouse))
Name: SNF2 histone linker PHD RING helicase
Synonyms: D230017O13Rik, 2610103K11Rik
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ndrg4 [MGI:2384590] (Mus musculus (mouse))
Name: N-myc downstream regulated gene 4
Synonyms: Ndr4, SMAP-8, D8Bwg1337e, Ndr1-rs
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Pkp4 [MGI:109281] (Mus musculus (mouse))
Name: plakophilin 4
Synonyms: p0071, Armrp, 5031422I09Rik, 9430019K17Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Iqgap1 [MGI:1352757] (Mus musculus (mouse))
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Lars [MGI:1913808] (Mus musculus (mouse))
Name: leucyl-tRNA synthetase
Synonyms: 2310045K21Rik, 3110009L02Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Prrc2b [MGI:1923304] (Mus musculus (mouse))
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 11,200,057 bp
  • G to T, chromosome 1 at 26,685,266 bp
  • A to G, chromosome 1 at 58,016,697 bp
  • G to T, chromosome 1 at 66,510,881 bp
  • A to T, chromosome 1 at 97,895,723 bp
  • A to C, chromosome 1 at 139,241,166 bp
  • T to A, chromosome 1 at 174,609,954 bp
  • A to T, chromosome 2 at 13,000,937 bp
  • C to A, chromosome 2 at 28,469,779 bp
  • G to T, chromosome 2 at 32,199,091 bp
  • T to C, chromosome 2 at 40,851,711 bp
  • A to G, chromosome 2 at 59,318,529 bp
  • G to A, chromosome 2 at 86,198,711 bp
  • A to T, chromosome 2 at 86,509,205 bp
  • A to T, chromosome 2 at 90,265,536 bp
  • A to C, chromosome 2 at 119,771,840 bp
  • A to T, chromosome 2 at 127,801,394 bp
  • A to G, chromosome 2 at 137,095,445 bp
  • A to T, chromosome 2 at 168,926,040 bp
  • A to G, chromosome 3 at 26,667,456 bp
  • T to C, chromosome 3 at 27,467,430 bp
  • A to G, chromosome 3 at 59,077,059 bp
  • G to A, chromosome 3 at 88,325,084 bp
  • A to G, chromosome 3 at 108,461,251 bp
  • G to A, chromosome 3 at 136,935,139 bp
  • T to A, chromosome 4 at 70,455,941 bp
  • G to T, chromosome 4 at 133,062,719 bp
  • C to T, chromosome 4 at 138,800,656 bp
  • A to T, chromosome 4 at 143,966,740 bp
  • T to C, chromosome 4 at 144,555,511 bp
  • T to G, chromosome 4 at 155,551,683 bp
  • A to T, chromosome 5 at 24,435,943 bp
  • C to T, chromosome 5 at 34,149,764 bp
  • T to A, chromosome 5 at 74,595,842 bp
  • A to G, chromosome 5 at 76,615,743 bp
  • A to T, chromosome 5 at 113,288,835 bp
  • A to G, chromosome 5 at 120,795,395 bp
  • A to T, chromosome 5 at 120,943,064 bp
  • A to G, chromosome 5 at 137,322,846 bp
  • A to T, chromosome 5 at 143,983,897 bp
  • C to A, chromosome 5 at 144,816,395 bp
  • A to G, chromosome 6 at 21,216,683 bp
  • A to T, chromosome 6 at 40,544,123 bp
  • C to A, chromosome 6 at 83,069,300 bp
  • A to T, chromosome 6 at 87,111,295 bp
  • C to A, chromosome 6 at 97,113,955 bp
  • T to G, chromosome 6 at 97,176,504 bp
  • A to T, chromosome 6 at 121,849,842 bp
  • A to C, chromosome 6 at 136,560,989 bp
  • A to G, chromosome 7 at 45,225,755 bp
  • G to A, chromosome 7 at 47,090,758 bp
  • A to C, chromosome 7 at 47,090,759 bp
  • T to C, chromosome 7 at 57,765,530 bp
  • A to T, chromosome 7 at 67,667,159 bp
  • A to G, chromosome 7 at 80,751,930 bp
  • G to A, chromosome 7 at 81,067,953 bp
  • A to T, chromosome 7 at 104,580,360 bp
  • G to A, chromosome 8 at 22,028,451 bp
  • A to G, chromosome 8 at 36,103,804 bp
  • T to C, chromosome 8 at 43,295,951 bp
  • A to T, chromosome 8 at 61,983,855 bp
  • A to T, chromosome 8 at 63,928,376 bp
  • T to A, chromosome 8 at 95,710,935 bp
  • T to A, chromosome 8 at 106,194,517 bp
  • T to C, chromosome 8 at 106,201,056 bp
  • T to C, chromosome 8 at 112,008,216 bp
  • T to G, chromosome 9 at 31,414,180 bp
  • T to C, chromosome 9 at 38,728,665 bp
  • A to T, chromosome 9 at 78,453,004 bp
  • T to C, chromosome 9 at 104,004,705 bp
  • A to C, chromosome 9 at 107,749,806 bp
  • A to G, chromosome 9 at 119,179,778 bp
  • G to A, chromosome 10 at 11,170,109 bp
  • T to A, chromosome 10 at 19,597,432 bp
  • T to C, chromosome 10 at 49,240,725 bp
  • A to T, chromosome 10 at 79,148,939 bp
  • C to T, chromosome 10 at 79,542,596 bp
  • G to T, chromosome 10 at 79,891,758 bp
  • T to C, chromosome 10 at 116,113,569 bp
  • C to T, chromosome 10 at 127,526,911 bp
  • G to T, chromosome 10 at 127,898,719 bp
  • T to C, chromosome 11 at 78,963,448 bp
  • T to G, chromosome 11 at 94,813,092 bp
  • T to A, chromosome 11 at 101,879,401 bp
  • C to A, chromosome 11 at 114,959,585 bp
  • T to A, chromosome 11 at 115,884,265 bp
  • T to G, chromosome 11 at 117,356,632 bp
  • T to C, chromosome 12 at 54,203,512 bp
  • C to T, chromosome 12 at 71,179,162 bp
  • A to T, chromosome 12 at 75,962,641 bp
  • C to A, chromosome 12 at 103,171,644 bp
  • T to A, chromosome 12 at 105,807,193 bp
  • C to G, chromosome 12 at 118,012,339 bp
  • A to G, chromosome 13 at 23,114,594 bp
  • C to T, chromosome 13 at 81,540,015 bp
  • G to A, chromosome 14 at 26,438,747 bp
  • T to C, chromosome 14 at 118,909,557 bp
  • C to T, chromosome 15 at 65,897,665 bp
  • A to G, chromosome 15 at 77,968,220 bp
  • T to C, chromosome 15 at 85,819,551 bp
  • A to T, chromosome 15 at 100,693,471 bp
  • T to C, chromosome 15 at 101,644,326 bp
  • T to C, chromosome 16 at 15,810,702 bp
  • A to T, chromosome 17 at 34,844,815 bp
  • G to A, chromosome 17 at 56,099,181 bp
  • A to G, chromosome 17 at 93,202,832 bp
  • A to C, chromosome 18 at 12,482,126 bp
  • C to T, chromosome 18 at 37,940,952 bp
  • A to T, chromosome 18 at 42,250,902 bp
  • A to G, chromosome 18 at 52,905,809 bp
  • G to C, chromosome 18 at 61,697,893 bp
  • C to T, chromosome 18 at 62,986,963 bp
  • A to T, chromosome 18 at 80,130,653 bp
  • G to A, chromosome 19 at 25,546,007 bp
  • G to C, chromosome 19 at 50,313,042 bp
  • T to A, chromosome 19 at 60,161,858 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0325 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038535-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.