Strain Name:
C57BL/6J-MtgxR0335Btlr/Mmmh
Stock Number:
038544-MU
Citation ID:
RRID:MMRRC_038544-MU
Other Names:
R0335 (G1), C57BL/6J-MtgxR0335Btlr
Major Collection:

Gene Information

Gene Symbol: Prkar2a [MGI:108025] (Mus musculus (mouse))
Name: protein kinase, cAMP dependent regulatory, type II alpha
Synonyms: 1110061A24Rik, RII(alpha)
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Slc1a2 [MGI:101931] (Mus musculus (mouse))
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms: GLT-1, 2900019G14Rik, 1700091C19Rik, GLT1, MGLT1, Eaat2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Erbb4 [MGI:104771] (Mus musculus (mouse))
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: Her4, ErbB4
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Crybg3 [MGI:2676311] (Mus musculus (mouse))
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Col18a1 [MGI:88451] (Mus musculus (mouse))
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Zmym6 [MGI:106505] (Mus musculus (mouse))
Name: zinc finger, MYM-type 6
Synonyms: Zfp258, 9330177P20Rik, D4Wsu24e
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Rnf38 [MGI:1920719] (Mus musculus (mouse))
Name: ring finger protein 38
Synonyms: 2610202O07Rik, 1700065B19Rik, Oip1
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 34,579,300 bp
  • C to A, chromosome 1 at 68,259,259 bp
  • T to A, chromosome 1 at 93,052,566 bp
  • T to C, chromosome 1 at 93,495,599 bp
  • T to A, chromosome 1 at 121,492,977 bp
  • C to T, chromosome 2 at 5,024,115 bp
  • C to T, chromosome 2 at 32,802,905 bp
  • T to A, chromosome 2 at 59,496,594 bp
  • T to A, chromosome 2 at 65,682,091 bp
  • G to C, chromosome 2 at 98,664,007 bp
  • C to T, chromosome 2 at 102,743,863 bp
  • G to A, chromosome 2 at 170,418,681 bp
  • T to C, chromosome 3 at 36,969,152 bp
  • T to C, chromosome 3 at 63,710,978 bp
  • T to A, chromosome 3 at 81,964,000 bp
  • T to A, chromosome 3 at 90,356,182 bp
  • T to A, chromosome 3 at 97,921,256 bp
  • T to A, chromosome 3 at 108,012,696 bp
  • T to A, chromosome 4 at 43,236,983 bp
  • A to G, chromosome 4 at 44,152,507 bp
  • A to G, chromosome 4 at 118,727,170 bp
  • G to A, chromosome 4 at 127,122,808 bp
  • G to A, chromosome 5 at 31,073,985 bp
  • A to G, chromosome 5 at 63,804,773 bp
  • T to C, chromosome 5 at 107,812,411 bp
  • T to G, chromosome 5 at 108,797,451 bp
  • T to A, chromosome 5 at 134,535,215 bp
  • A to G, chromosome 5 at 149,050,631 bp
  • T to A, chromosome 6 at 4,531,956 bp
  • T to C, chromosome 6 at 5,491,138 bp
  • T to C, chromosome 6 at 73,069,399 bp
  • T to C, chromosome 6 at 90,022,659 bp
  • G to T, chromosome 6 at 114,480,232 bp
  • C to T, chromosome 6 at 132,957,829 bp
  • A to T, chromosome 7 at 26,394,136 bp
  • T to A, chromosome 7 at 44,864,622 bp
  • T to C, chromosome 7 at 130,196,249 bp
  • T to G, chromosome 8 at 48,232,105 bp
  • A to G, chromosome 8 at 75,064,392 bp
  • A to T, chromosome 8 at 95,772,000 bp
  • T to C, chromosome 8 at 117,445,366 bp
  • G to T, chromosome 9 at 3,001,048 bp
  • T to A, chromosome 9 at 18,815,994 bp
  • G to A, chromosome 9 at 31,091,324 bp
  • T to G, chromosome 9 at 32,259,760 bp
  • A to G, chromosome 9 at 37,522,760 bp
  • T to C, chromosome 9 at 44,353,838 bp
  • A to T, chromosome 9 at 60,677,095 bp
  • C to A, chromosome 9 at 64,118,887 bp
  • A to T, chromosome 9 at 108,719,258 bp
  • A to T, chromosome 10 at 75,657,049 bp
  • G to A, chromosome 10 at 77,059,363 bp
  • T to C, chromosome 10 at 107,708,728 bp
  • T to C, chromosome 11 at 58,625,740 bp
  • C to T, chromosome 11 at 67,662,052 bp
  • G to A, chromosome 11 at 70,001,035 bp
  • T to C, chromosome 11 at 99,022,955 bp
  • C to A, chromosome 11 at 115,624,206 bp
  • G to T, chromosome 12 at 69,353,803 bp
  • A to G, chromosome 12 at 70,453,256 bp
  • A to T, chromosome 12 at 109,038,934 bp
  • T to C, chromosome 12 at 114,406,674 bp
  • G to A, chromosome 13 at 24,073,983 bp
  • A to T, chromosome 13 at 24,887,877 bp
  • T to C, chromosome 13 at 32,848,656 bp
  • T to A, chromosome 13 at 95,635,633 bp
  • A to G, chromosome 14 at 12,301,266 bp
  • A to C, chromosome 14 at 20,320,734 bp
  • T to A, chromosome 14 at 20,688,715 bp
  • C to T, chromosome 14 at 33,359,108 bp
  • T to A, chromosome 14 at 34,578,651 bp
  • T to A, chromosome 14 at 34,692,324 bp
  • T to A, chromosome 15 at 10,539,963 bp
  • G to T, chromosome 15 at 11,311,058 bp
  • T to A, chromosome 15 at 21,578,549 bp
  • T to C, chromosome 15 at 89,583,966 bp
  • A to T, chromosome 16 at 59,544,140 bp
  • C to T, chromosome 16 at 79,024,742 bp
  • A to G, chromosome 17 at 17,911,112 bp
  • A to G, chromosome 17 at 28,228,069 bp
  • T to A, chromosome 17 at 28,886,878 bp
  • A to C, chromosome 17 at 37,530,642 bp
  • G to A, chromosome 17 at 78,827,946 bp
  • A to T, chromosome 17 at 83,587,737 bp
  • G to A, chromosome 17 at 88,015,613 bp
  • C to T, chromosome 18 at 77,986,472 bp
  • G to T, chromosome 19 at 7,281,828 bp
  • A to T, chromosome 19 at 10,980,210 bp
  • A to G, chromosome 19 at 53,236,828 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0335 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038544-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.