Strain Name:
C57BL/6J-MtgxR0376Btlr/Mmmh
Stock Number:
038582-MU
Citation ID:
RRID:MMRRC_038582-MU
Other Names:
R0376 (G1), C57BL/6J-MtgxR0376Btlr
Major Collection:

Gene Information

Arhgap10
Name: Rho GTPase activating protein 10
Synonyms: A930033B01Rik, PSGAP-m, PSGAP-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 78514
Homologene: 12695
Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Spag7
Name: sperm associated antigen 7
Synonyms: Fsa1l, FSA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216873
Homologene: 3595
Tead3
Name: TEA domain family member 3
Synonyms: TEAD-3, TEF-5, ETFR-1, Tcf13r2, DTEF-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 21678
Homologene: 81821
U2surp
Name: U2 snRNP-associated SURP domain containing
Synonyms: 2610101N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 67958
Homologene: 13964
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12695
Homologene: 72199
Ankrd12
Name: ankyrin repeat domain 12
Synonyms: ANCO-2, 2900001A12Rik, GAC-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 106585
Homologene: 9059
Ints14
Name: integrator complex subunit 14
Synonyms: 2010321M09Rik, Vwa9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 69882
VEGA: 9
Homologene: 12774
Kif13b
Name: kinesin family member 13B
Synonyms: 5330429L19Rik, N-3 kinesin, GAKIN, C130021D12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 16554
VEGA: 14
Homologene: 9073
Flnb
Name: filamin, beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 286940
HGNC: HGNC:3755
Homologene: 37480
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100683
Homologene: 39246
Rbm28
Name: RNA binding motif protein 28
Synonyms: 2810480G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 68272
Homologene: 135952
Zc3hc1
Name: zinc finger, C3HC type 1
Synonyms: 1110054L24Rik, Nipa, HSPC216
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232679
Homologene: 32315
Als2
Name: alsin Rho guanine nucleotide exchange factor
Synonyms: Als2cr6, Alsin, 3222402C23Rik, 9430073A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74018
HGNC: HGNC:443
Homologene: 23264
Mfn2
Name: mitofusin 2
Synonyms: hypertension related protein 1, D630023P19Rik, Fzo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 170731
Homologene: 8915
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 72745
Homologene: 14519
Rbm15
Name: RNA binding motif protein 15
Synonyms: C230088J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229700
Homologene: 23383
Snrnp40
Name: small nuclear ribonucleoprotein 40 (U5)
Synonyms: Wdr57, 0610009C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 66585
Homologene: 3538
Bcr
Name: breakpoint cluster region
Synonyms: 5133400C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 110279
VEGA: 10
HGNC: HGNC:1014
Homologene: 3192
Anxa5
Name: annexin A5
Synonyms: annexin V, Anx5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 11747
HGNC: HGNC:543
Homologene: 20312
Mkln1
Name: muskelin 1, intracellular mediator containing kelch motifs
Synonyms: A130067F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 27418
HGNC: HGNC:7109
Homologene: 8305
Sugp1
Name: SURP and G patch domain containing 1
Synonyms: Sf4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 70616
Homologene: 12360
Pwwp2a
Name: PWWP domain containing 2A
Synonyms: 4631424J17Rik, D930040F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 70802
Homologene: 19687
Atp2a3
Name: ATPase, Ca++ transporting, ubiquitous
Synonyms: Serca3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 53313
HGNC: HGNC:813
Homologene: 69131
Lama2
Name: laminin, alpha 2
Synonyms: mer, merosin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Pus3
Name: pseudouridine synthase 3
Synonyms: 2610020J05Rik, 5730412F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 67049
Homologene: 11339
Plcb2
Name: phospholipase C, beta 2
Synonyms: B230205M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18796
HGNC: HGNC:9055
Homologene: 20957
Rhobtb2
Name: Rho-related BTB domain containing 2
Synonyms: Dbc2, E130206H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 246710
VEGA: 14
Homologene: 22873
Krt71
Name: keratin 71
Synonyms: Cu, Krt2-6g, mK6irs1, Cal4, Ca, mK6irs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 56735
Homologene: 88864
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, Cav2.2, alpha(1B)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Zc3h7a
Name: zinc finger CCCH type containing 7 A
Synonyms: Zc3h7, A430104C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 106205
Homologene: 8563
Frmd4a
Name: FERM domain containing 4A
Synonyms: 2700017I06Rik, C230040M21Rik, Gm13190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 209630
Homologene: 9971
Pcnx
Name: pecanex homolog
Synonyms: 2900024E21Rik, 3526401J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 54604
VEGA: 12
Homologene: 40997
Adam6a
Name: a disintegrin and metallopeptidase domain 6A
Synonyms: Adam6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238406
HGNC: HGNC:213
Homologene: 128362
Zfp93
Name: zinc finger protein 93
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22755
HGNC: null
Homologene: 117959
Ikzf4
Name: IKAROS family zinc finger 4
Synonyms: A630026H08Rik, Zfpn1a4, Eos
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22781
Homologene: 69103
Cyp2j6
Name: cytochrome P450, family 2, subfamily j, polypeptide 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 13110
HGNC: HGNC:2634
Homologene: 68091
Ggn
Name: gametogenetin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 243897
Homologene: 45139
Tecpr1
Name: tectonin beta-propeller repeat containing 1
Synonyms: 2210010N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 70381
Homologene: 9120
Sun1
Name: Sad1 and UNC84 domain containing 1
Synonyms: 4632417G13Rik, Unc84a, 5730434D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 77053
Homologene: 11544
Zfp366
Name: zinc finger protein 366
Synonyms: DC-SCRIPT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 238803
Homologene: 17637
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235086
Homologene: 19472
Hexdc
Name: hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 238023
Homologene: 68120
Rimbp2
Name: RIMS binding protein 2
Synonyms: A930033C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231760
Homologene: 14672
Scgb1b27
Name: secretoglobin, family 1B, member 27
Synonyms: Abpa27, Abpa, Tcp, Abp, Sal-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11354
HGNC: null
Homologene: 114479
Ttbk2
Name: tau tubulin kinase 2
Synonyms: 2610507N02Rik, B930008N24Rik, TTK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 140810
Homologene: 62795
Prkcsh
Name: protein kinase C substrate 80K-H
Synonyms: 80K-H, hepatocystin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 19089
VEGA: 9
HGNC: HGNC:9411
Homologene: 2056
Cyp3a11
Name: cytochrome P450, family 3, subfamily a, polypeptide 11
Synonyms: IIIAm1, Cyp3a, Pcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 13112
HGNC: HGNC:2638
Homologene: 133568
Prr14
Name: proline rich 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233895
Homologene: 11424
Slc40a1
Name: solute carrier family 40 (iron-regulated transporter), member 1
Synonyms: IREG1, Pcm, Dusg, Slc11a3, metal transporting protein 1, MTP1, Ol5, ferroportin1, FPN1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 53945
Homologene: 40959
Spaca9
Name: sperm acrosome associated 9
Synonyms: Mast, 1700026L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 69987
HGNC: HGNC:1367
Homologene: 10346
Tcp11l1
Name: t-complex 11 like 1
Synonyms: C130096D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 320554
Homologene: 41260
Tldc2
Name: TBC/LysM associated domain containing 2
Synonyms: LOC383766, Gm1332
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 383766
Homologene: 31961
1700021F07Rik
Name: RIKEN cDNA 1700021F07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 72221
Homologene: 82371
Plppr4
Name: phospholipid phosphatase related 4
Synonyms: PRG-1, Lppr4, D3Bwg0562e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229791
Homologene: 8895
Olfr450
Name: olfactory receptor 450
Synonyms: GA_x6K02T2P3E9-4742413-4741481, MOR257-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 258437
HGNC: null
Homologene: 51720
Camp
Name: cathelicidin antimicrobial peptide
Synonyms: Cnlp, MCLP, CAP18, FALL39, Cramp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12796
HGNC: HGNC:1472
Homologene: 110678
H60c
Name: histocompatibility 60c
Synonyms: Gm9491, 4632413I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 670558
VEGA: 10
HGNC: null
Homologene: 120804
Gabrg2
Name: gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2
Synonyms: Gabrg-2, GABAA-R, gamma2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14406
HGNC: HGNC:4087
Homologene: 22443
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 45,912,491 bp
  • A to G, chromosome 1 at 59,215,565 bp
  • G to T, chromosome 2 at 4,572,387 bp
  • A to G, chromosome 2 at 24,659,003 bp
  • A to G, chromosome 2 at 28,693,660 bp
  • C to A, chromosome 2 at 104,697,505 bp
  • T to C, chromosome 2 at 118,717,240 bp
  • A to G, chromosome 2 at 120,777,581 bp
  • T to C, chromosome 2 at 157,095,305 bp
  • G to A, chromosome 2 at 173,528,327 bp
  • T to C, chromosome 3 at 36,460,488 bp
  • T to C, chromosome 3 at 107,330,938 bp
  • G to T, chromosome 3 at 117,323,091 bp
  • T to A, chromosome 4 at 96,526,023 bp
  • T to A, chromosome 4 at 98,568,987 bp
  • C to G, chromosome 4 at 130,378,043 bp
  • C to T, chromosome 4 at 147,885,526 bp
  • C to T, chromosome 5 at 128,803,861 bp
  • A to G, chromosome 5 at 139,226,699 bp
  • A to T, chromosome 5 at 144,207,476 bp
  • A to G, chromosome 5 at 144,816,339 bp
  • A to C, chromosome 5 at 145,862,452 bp
  • A to G, chromosome 6 at 29,158,928 bp
  • G to C, chromosome 6 at 30,372,790 bp
  • A to T, chromosome 6 at 31,478,018 bp
  • A to T, chromosome 6 at 42,818,292 bp
  • C to T, chromosome 7 at 24,275,861 bp
  • T to C, chromosome 7 at 29,173,022 bp
  • C to T, chromosome 7 at 34,021,897 bp
  • T to C, chromosome 7 at 80,723,879 bp
  • C to T, chromosome 7 at 127,476,643 bp
  • A to G, chromosome 8 at 70,052,638 bp
  • T to C, chromosome 8 at 77,450,824 bp
  • A to G, chromosome 9 at 22,010,251 bp
  • A to G, chromosome 9 at 27,334,582 bp
  • A to T, chromosome 9 at 35,566,422 bp
  • A to G, chromosome 9 at 64,983,990 bp
  • A to T, chromosome 9 at 79,693,494 bp
  • C to T, chromosome 9 at 95,484,443 bp
  • C to T, chromosome 9 at 109,848,399 bp
  • T to C, chromosome 10 at 3,260,435 bp
  • T to C, chromosome 10 at 27,015,546 bp
  • G to T, chromosome 10 at 75,145,327 bp
  • T to C, chromosome 10 at 128,632,756 bp
  • C to T, chromosome 11 at 41,916,315 bp
  • T to A, chromosome 11 at 43,704,672 bp
  • T to C, chromosome 11 at 70,669,190 bp
  • T to A, chromosome 11 at 72,982,702 bp
  • T to A, chromosome 11 at 121,218,165 bp
  • C to T, chromosome 12 at 81,974,579 bp
  • T to C, chromosome 12 at 113,544,690 bp
  • A to G, chromosome 13 at 84,292,383 bp
  • T to C, chromosome 13 at 99,234,251 bp
  • G to A, chromosome 14 at 7,946,014 bp
  • T to C, chromosome 14 at 64,757,404 bp
  • A to T, chromosome 14 at 69,796,735 bp
  • A to C, chromosome 15 at 101,738,070 bp
  • T to C, chromosome 16 at 11,156,202 bp
  • A to C, chromosome 17 at 28,341,365 bp
  • T to A, chromosome 17 at 66,053,009 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0376 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038582-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.