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Strain Name:
C57BL/6J-MtgxR0377Btlr/Mmmh
Stock Number:
038583-MU
Citation ID:
RRID:MMRRC_038583-MU
Other Names:
R0377 (G1), C57BL/6J-MtgxR0377Btlr
Major Collection:

Strain Information

Dntt
Name: deoxynucleotidyltransferase, terminal
Synonyms: Tdt
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21673
VEGA: 19
HGNC: HGNC:2983
Homologene: 3014
Cep55
Name: centrosomal protein 55
Synonyms: 2700032M20Rik, 1200008O12Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74107
VEGA: 19
HGNC: HGNC:1161
Homologene: 10019
U2surp
Name: U2 snRNP-associated SURP domain containing
Synonyms: 2610101N10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67958
Homologene: 13964
Stk4
Name: serine/threonine kinase 4
Synonyms: Ysk3, Kas-2, Mst1, sterile 20-like kinase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58231
Homologene: 55965
Fbxo31
Name: F-box protein 31
Synonyms: 2310046N15Rik, Fbx14, Fbxo14, 1110003O08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76454
Homologene: 11684
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Ptpn23
Name: protein tyrosine phosphatase, non-receptor type 23
Synonyms: PTP-TD14
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 104831
Homologene: 135706
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 74,612,730 bp
  • A to T, chromosome 1 at 116,292,529 bp
  • T to C, chromosome 1 at 175,663,694 bp
  • G to A, chromosome 2 at 29,761,791 bp
  • A to T, chromosome 2 at 86,553,583 bp
  • A to T, chromosome 2 at 126,125,000 bp
  • A to G, chromosome 2 at 128,641,340 bp
  • T to C, chromosome 2 at 164,096,800 bp
  • T to A, chromosome 3 at 97,662,317 bp
  • A to G, chromosome 3 at 153,896,890 bp
  • G to A, chromosome 4 at 4,005,546 bp
  • T to A, chromosome 4 at 31,985,731 bp
  • T to C, chromosome 4 at 34,044,267 bp
  • C to T, chromosome 4 at 43,674,005 bp
  • A to G, chromosome 4 at 103,048,259 bp
  • T to A, chromosome 4 at 125,046,382 bp
  • C to G, chromosome 4 at 130,378,043 bp
  • A to T, chromosome 5 at 36,604,567 bp
  • T to C, chromosome 5 at 45,693,817 bp
  • A to T, chromosome 5 at 86,490,751 bp
  • A to T, chromosome 5 at 87,517,376 bp
  • C to T, chromosome 5 at 128,803,861 bp
  • G to A, chromosome 5 at 137,290,928 bp
  • A to T, chromosome 6 at 52,202,646 bp
  • A to G, chromosome 6 at 73,121,992 bp
  • A to G, chromosome 6 at 121,857,361 bp
  • A to G, chromosome 6 at 136,750,917 bp
  • C to A, chromosome 7 at 25,285,799 bp
  • A to T, chromosome 7 at 30,574,193 bp
  • T to A, chromosome 7 at 39,536,797 bp
  • A to G, chromosome 8 at 3,541,501 bp
  • T to C, chromosome 8 at 24,747,541 bp
  • T to A, chromosome 8 at 105,878,872 bp
  • G to A, chromosome 8 at 121,559,102 bp
  • C to T, chromosome 9 at 95,484,443 bp
  • T to A, chromosome 9 at 104,081,692 bp
  • A to T, chromosome 9 at 110,388,132 bp
  • A to G, chromosome 10 at 63,127,622 bp
  • G to A, chromosome 10 at 79,967,502 bp
  • G to A, chromosome 10 at 80,702,840 bp
  • T to A, chromosome 10 at 102,545,649 bp
  • G to A, chromosome 11 at 61,069,396 bp
  • A to G, chromosome 11 at 61,741,000 bp
  • C to A, chromosome 11 at 94,375,096 bp
  • A to G, chromosome 11 at 97,055,934 bp
  • T to G, chromosome 11 at 117,149,140 bp
  • G to A, chromosome 12 at 76,795,156 bp
  • G to A, chromosome 12 at 80,706,436 bp
  • C to T, chromosome 12 at 81,974,579 bp
  • T to C, chromosome 12 at 111,629,029 bp
  • T to C, chromosome 13 at 41,049,348 bp
  • T to G, chromosome 14 at 78,299,912 bp
  • T to C, chromosome 15 at 12,160,591 bp
  • C to A, chromosome 15 at 50,831,778 bp
  • T to A, chromosome 15 at 97,246,380 bp
  • G to A, chromosome 16 at 18,249,703 bp
  • A to G, chromosome 16 at 23,431,284 bp
  • A to T, chromosome 17 at 24,530,045 bp
  • T to A, chromosome 17 at 39,409,944 bp
  • G to A, chromosome 17 at 53,500,462 bp
  • T to A, chromosome 17 at 55,938,671 bp
  • G to A, chromosome 18 at 7,127,415 bp
  • A to G, chromosome 19 at 36,989,002 bp
  • T to A, chromosome 19 at 38,071,889 bp
  • G to A, chromosome 19 at 41,047,627 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0377 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038583-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.