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Strain Name:
C57BL/6J-MtgxR0414Btlr/Mmmh
Stock Number:
038616-MU
Citation ID:
RRID:MMRRC_038616-MU
Other Names:
R0414 (G1), C57BL/6J-MtgxR0414Btlr
Major Collection:

Strain Information

Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269473
Homologene: 8882
Lrrn3
Name: leucine rich repeat protein 3, neuronal
Synonyms: NLRR-3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16981
VEGA: 12
Homologene: 36315
Nagk
Name: N-acetylglucosamine kinase
Synonyms: GlcNAc kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56174
Homologene: 9720
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Eif2s2
Name: eukaryotic translation initiation factor 2 subunit 2 beta
Synonyms: EIF2B, EIF2, 2810026E11Rik, 38kDa, D2Ertd303e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67204
HGNC: HGNC:3266
Homologene: 2904
Fbxo22
Name: F-box protein 22
Synonyms: 1600016C16Rik, 0610033L19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71999
Homologene: 12433
Ptpn5
Name: protein tyrosine phosphatase, non-receptor type 5
Synonyms: Step
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19259
HGNC: HGNC:9657
Homologene: 8423
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,537,328 bp
  • C to A, chromosome 1 at 80,535,933 bp
  • T to C, chromosome 1 at 86,305,629 bp
  • T to C, chromosome 1 at 150,715,822 bp
  • T to C, chromosome 1 at 163,968,321 bp
  • T to C, chromosome 2 at 26,843,416 bp
  • A to G, chromosome 2 at 34,693,427 bp
  • T to A, chromosome 2 at 65,525,982 bp
  • A to G, chromosome 2 at 89,023,146 bp
  • T to C, chromosome 2 at 91,875,739 bp
  • A to G, chromosome 2 at 154,884,461 bp
  • T to C, chromosome 3 at 88,632,268 bp
  • C to T, chromosome 3 at 92,825,713 bp
  • A to G, chromosome 3 at 104,494,056 bp
  • A to G, chromosome 3 at 142,507,913 bp
  • A to G, chromosome 3 at 146,476,188 bp
  • T to A, chromosome 4 at 109,366,480 bp
  • GAGAAGAAG to GAGAAG, chromosome 4 at 134,072,636 bp
  • T to C, chromosome 5 at 38,105,005 bp
  • A to T, chromosome 5 at 103,649,490 bp
  • A to G, chromosome 5 at 123,287,413 bp
  • A to G, chromosome 5 at 129,504,051 bp
  • C to T, chromosome 6 at 83,797,267 bp
  • T to C, chromosome 6 at 120,216,565 bp
  • T to C, chromosome 6 at 121,856,554 bp
  • T to C, chromosome 6 at 125,107,480 bp
  • T to C, chromosome 6 at 129,326,850 bp
  • AC to ACC, chromosome 7 at 3,296,760 bp
  • T to C, chromosome 7 at 44,846,059 bp
  • G to A, chromosome 7 at 47,083,136 bp
  • A to T, chromosome 7 at 90,189,198 bp
  • A to G, chromosome 8 at 25,767,997 bp
  • A to C, chromosome 8 at 34,853,309 bp
  • A to G, chromosome 8 at 69,882,993 bp
  • A to G, chromosome 8 at 80,800,289 bp
  • T to A, chromosome 9 at 26,765,104 bp
  • T to A, chromosome 9 at 55,223,626 bp
  • T to A, chromosome 10 at 128,671,457 bp
  • T to C, chromosome 11 at 3,819,932 bp
  • C to T, chromosome 11 at 44,924,470 bp
  • T to A, chromosome 11 at 46,161,908 bp
  • T to C, chromosome 11 at 69,499,238 bp
  • A to G, chromosome 11 at 83,121,270 bp
  • T to G, chromosome 11 at 119,499,571 bp
  • A to G, chromosome 11 at 120,613,086 bp
  • T to A, chromosome 12 at 41,453,940 bp
  • T to C, chromosome 12 at 72,094,145 bp
  • T to A, chromosome 13 at 6,593,210 bp
  • A to G, chromosome 13 at 23,764,158 bp
  • T to C, chromosome 14 at 47,276,588 bp
  • T to A, chromosome 15 at 31,617,002 bp
  • C to A, chromosome 15 at 85,045,689 bp
  • A to G, chromosome 16 at 85,877,906 bp
  • C to G, chromosome 16 at 94,663,842 bp
  • T to A, chromosome 17 at 47,788,299 bp
  • A to C, chromosome 17 at 50,607,955 bp
  • G to T, chromosome 17 at 71,899,286 bp
  • A to T, chromosome 18 at 20,088,354 bp
  • A to G, chromosome 18 at 65,306,159 bp
  • T to C, chromosome 19 at 4,607,642 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0414 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038616-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.