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Strain Name:
C57BL/6J-MtgxR0419Btlr/Mmmh
Stock Number:
038621-MU
Citation ID:
RRID:MMRRC_038621-MU
Other Names:
R0419 (G1), C57BL/6J-MtgxR0419Btlr
Major Collection:

Strain Information

Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Arid1a
Name: AT-rich interaction domain 1A
Synonyms: Osa1, 1110030E03Rik, Smarcf1, BAF250a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 93760
Homologene: 21216
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Zfp280d
Name: zinc finger protein 280D
Synonyms: Suhw4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235469
Homologene: 17151
Iqgap2
Name: IQ motif containing GTPase activating protein 2
Synonyms: 4933417J23Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 544963
VEGA: 13
HGNC: HGNC:6111
Homologene: 101543
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 107,376,860 bp
  • A to C, chromosome 2 at 13,469,763 bp
  • T to A, chromosome 2 at 13,469,764 bp
  • A to T, chromosome 2 at 84,691,243 bp
  • A to T, chromosome 2 at 85,849,967 bp
  • A to T, chromosome 2 at 146,428,672 bp
  • A to T, chromosome 2 at 169,582,953 bp
  • T to C, chromosome 3 at 10,180,763 bp
  • A to T, chromosome 3 at 15,548,596 bp
  • G to T, chromosome 3 at 55,819,294 bp
  • C to A, chromosome 3 at 107,281,911 bp
  • G to A, chromosome 4 at 62,326,136 bp
  • T to G, chromosome 4 at 125,053,527 bp
  • G to T, chromosome 4 at 133,681,124 bp
  • G to T, chromosome 5 at 30,915,022 bp
  • A to G, chromosome 5 at 128,993,577 bp
  • C to T, chromosome 5 at 129,658,238 bp
  • A to T, chromosome 6 at 24,100,293 bp
  • T to C, chromosome 6 at 41,034,347 bp
  • A to G, chromosome 6 at 136,840,145 bp
  • A to G, chromosome 7 at 41,573,445 bp
  • T to C, chromosome 8 at 13,551,842 bp
  • A to T, chromosome 8 at 25,937,618 bp
  • T to C, chromosome 8 at 36,583,586 bp
  • T to C, chromosome 8 at 106,134,675 bp
  • A to G, chromosome 9 at 15,992,256 bp
  • A to G, chromosome 9 at 58,990,180 bp
  • G to A, chromosome 9 at 61,926,405 bp
  • A to G, chromosome 9 at 65,557,052 bp
  • T to C, chromosome 9 at 66,446,074 bp
  • T to C, chromosome 9 at 72,312,237 bp
  • G to T, chromosome 10 at 50,748,926 bp
  • T to C, chromosome 10 at 75,412,883 bp
  • T to A, chromosome 10 at 77,042,908 bp
  • A to T, chromosome 10 at 93,682,429 bp
  • T to C, chromosome 11 at 11,934,207 bp
  • T to C, chromosome 11 at 70,615,723 bp
  • A to T, chromosome 12 at 18,533,423 bp
  • A to G, chromosome 12 at 21,297,799 bp
  • T to C, chromosome 12 at 58,267,665 bp
  • T to A, chromosome 12 at 91,537,869 bp
  • A to G, chromosome 13 at 36,537,311 bp
  • G to T, chromosome 13 at 56,632,193 bp
  • A to C, chromosome 13 at 95,689,699 bp
  • A to T, chromosome 14 at 52,204,060 bp
  • A to T, chromosome 14 at 59,406,744 bp
  • C to T, chromosome 14 at 96,381,789 bp
  • T to C, chromosome 16 at 19,673,552 bp
  • T to A, chromosome 16 at 32,476,436 bp
  • T to C, chromosome 16 at 85,866,642 bp
  • T to C, chromosome 17 at 33,950,790 bp
  • T to C, chromosome 17 at 34,858,509 bp
  • C to T, chromosome 17 at 56,708,219 bp
  • T to C, chromosome 17 at 67,791,610 bp
  • T to C, chromosome 18 at 44,031,867 bp
  • T to A, chromosome 19 at 36,945,229 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0419 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038621-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.